Vaccine composition for preventing and treating porcine circovirus type 2, haemophilus parasuis and mycoplasma hyopneumoniae infection and preparation method thereof

A technology of mycoplasma hyopneumoniae and vaccine composition, which is applied in the direction of viral antigen components, antiviral agents, bacterial antigen components, etc., and can solve problems such as impact, increased number of immunizations, no literature and patents, etc.

Active Publication Date: 2013-05-08
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the interference or influence of various antigens in the combined vaccine (vaccine composition), such as the double vaccine or the triple vaccine, there is no porcine circovirus disease, parasuis philophilus disease, There are no related documents and patents for the product of the triple vaccine for mycoplasma pneumoniae in swine
Therefore, at present, for the prevention of co-infection or secondary infection of porcine circovirus, Haemophilus parasuis and Mycoplasma hyopneumoniae, only inactivated vaccines for porcine circovirus disease, inactivated vaccine for Haemophilus parasuis disease, mycoplasma suis Pneumonia vaccine is immunized three times to prevent and control, resulting in complex immunization procedures, increased immunization times, increased immunization costs, increased immune stress times of pigs, and affected pig growth performance

Method used

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  • Vaccine composition for preventing and treating porcine circovirus type 2, haemophilus parasuis and mycoplasma hyopneumoniae infection and preparation method thereof
  • Vaccine composition for preventing and treating porcine circovirus type 2, haemophilus parasuis and mycoplasma hyopneumoniae infection and preparation method thereof
  • Vaccine composition for preventing and treating porcine circovirus type 2, haemophilus parasuis and mycoplasma hyopneumoniae infection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of three antigens in the vaccine composition of embodiment 1, porcine circovirus type 2, Haemophilus parasuis, and Mycoplasma hyopneumoniae

[0043] 1.1 Preparation of bacteria (virus) species for production

[0044] Preparation of porcine circovirus type 2 SH: the virus seed PCV2SH strain was diluted 1:9 with MEM liquid medium (prepared with MEM dry powder medium purchased from Invitrogen Company of the United States according to the instructions), and then divided into 5% of the volume of cell culture medium. % inoculated in PK15 (ATCC, preservation number CCL-33) monolayer cells, adsorbed at 37°C for 30 minutes, added cell maintenance solution (4% calf serum and 2mmol / L D-glucosamine hydrochloride were added to MEM liquid medium) ), cultivated at 37°C for 4 days, freeze-thawed 2-3 times, harvested the virus, and the virus titer was 10 6.5 TCID 50 / ml.

[0045] Preparation of Haemophilus parasuis strains: Streak inoculation of Haemophilus parasuis type ...

Embodiment 2

[0092] Embodiment 2, the preparation of porcine circovirus antigen, Haemophilus parasuis antigen, mycoplasma swine pneumonia antigen vaccine composition:

[0093] In order to compare the immune effects of vaccine compositions with different antigen contents, 4 kinds of adjuvants were selected to prepare the vaccine compositions, namely Montanide TM Gel (produced by SEPPIC, France), aluminum glue, white oil, squalene, each adjuvant was used to prepare porcine circovirus antigen, Haemophilus parasuis antigen, mycoplasma pneumoniae antigen vaccine composition and porcine round Circovirus disease, Haemophilus parasuis disease, and mycoplasma pneumoniae single vaccine, in which two vaccines with gradient antigen content were prepared for each single vaccine, and the antigen content of a single vaccine was the same as that of porcine circovirus disease, Haemophilus parasuis disease, mycoplasma pneumonia vaccine composition of the present invention (or claim the vaccine composition ...

Embodiment 3

[0133] Embodiment 3, porcine circovirus type 2 antigen, Haemophilus parasuis antigen, Mycoplasma hyopneumoniae antigen vaccine composition and the vaccine composition vaccine of each individual antigen The comparative test of immune effect on pigs

[0134] 3.1 Piglets immunized with vaccines for the test

[0135] Vaccine compositions (vaccine 1, vaccine 1', vaccine 8, vaccine 15, vaccine 22) of different adjuvants porcine circovirus type 2, Haemophilus parasuis, and mycoplasma hyopneumoniae prepared by the method described in Example 2 and Porcine circovirus type 2 inactivated vaccines (vaccine 2, vaccine 3, vaccine 9, vaccine 10, vaccine 16, vaccine 17, vaccine 23, vaccine 24), inactivated vaccines for porcine haemophilus parasuis (vaccine 4, vaccine 5. Vaccine 11, Vaccine 12, Vaccine 18, Vaccine 19, Vaccine 25, Vaccine 26), Inactivated Vaccine of Swine Mycoplasma Pneumonia (Vaccine 6, Vaccine 6', Vaccine 7, Vaccine 7', Vaccine 13, Vaccine 14, Vaccine 20, Vaccine 21, Vaccine...

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Abstract

The invention provides a vaccine composition for preventing and treating porcine circovirus type 2, haemophilus parasuis and mycoplasma hyopneumoniae infection. The vaccine composition comprises an inactivated porcine circovirus type 2 antigen, inactivated haemophilus parasuis, inactivated mycoplasma hyopneumoniae and a vaccine adjuvant. The vaccine composition disclosed by the invention can realize the aim of preventing three diseases including a porcine circovirus disease, mycoplasma pneumonia, a haemophilus parasuis disease by one injection of the vaccine; the content of antigen is 1/2 of the content of a common single-vaccine antigen when the vaccine composition disclosed by the invention is prepared by mixing the three antigens; and compared with the existing condition that three injections of single vaccine are injected to prevent three infectious diseases, the technical scheme disclosed by the invention is economical and practical, reduces the production cost, simplifies an immune procedure and reduces the epidemic prevention cost.

Description

technical field [0001] The invention relates to a vaccine composition for preventing and treating porcine circovirus type 2, Haemophilus parasuis and mycoplasma hyopneumoniae infection, belonging to the field of veterinary vaccines. Background technique [0002] Porcine circovirus (PCV) is the smallest animal virus ever discovered. PCV is known to have two serotypes, PCV1 and PCV2. PCV1 is a non-pathogenic virus. PCV2 is a pathogenic virus that is the main cause of multisystemic wasting syndrome (PMWS) in weaned piglets. In addition to PMWS, diseases such as PDNS (Porcine Dermatitis and Nephrotic Syndrome), PNP (Proliferative Necrotizing Pneumonia), PRDC (Porcine Respiratory Disease Syndrome), reproductive disorders, congenital tremors, and enteritis are also associated with PCV2 infection. [0003] Haemophilus parasuis is also known as multiple fibrinous serositis and arthritis, also known as Glaser's disease. It is caused by Haemophilus parasuis. Haemophilus parasuis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/04A61P31/20A61K39/02A61K39/102A61K39/12
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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