Vaccine composition and preparation method and application thereof

A vaccine composition and antigen technology, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, and viral antigen components, etc., can solve the problem of no prevention of duck hemorrhagic oophoritis, simplify the immunization procedure, and reduce the egg production rate. Reduced, reduced or even discontinued effects

Inactive Publication Date: 2015-03-25
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The duck plague vaccine prepared by the invention can be used to prevent duck plague, but it can only prevent duck plague, and has no preventive effect on duck hemorrhagic ovariitis

Method used

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  • Vaccine composition and preparation method and application thereof
  • Vaccine composition and preparation method and application thereof
  • Vaccine composition and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1 duck Tembusu virus, duck plague virus antigen

[0034] 1.1 Preparation of poisonous seeds for production

[0035] 1.1.1 Establishment of seed batches for duck Tembusu virus production

[0036] Muscovy duck embryo-adapted virus of duck Tembusu virus WR strain was established by passage of muscovy duck embryos. Duck Tembusu virus WR strain can also be used as a virulent test.

[0037] Duck Tembusu virus WR strain was subcultured for 7 consecutive times by using susceptible duck embryos, and the E1-E7 generation seed virus was identified. The identification results showed that the virus was qualified in terms of valence, virulence, specificity, immunogenicity, safety and purity. At the same time, the duck Tembusu virus inactivated vaccine was made with the duck Tembusu virus WR strain, which can produce complete protection after immunizing adult ducks.

[0038] Therefore, the duck Tembusu virus WR strain can be used to establish seed lot...

Embodiment 2

[0058] The preparation of two kinds of virus single vaccines of embodiment 2 and double combination vaccine

[0059] 2.1 Preparation of double seedlings

[0060] 2.1.1 Preparation of oil phase Take 94 parts of white oil for injection, add Siben-806 parts, mix, add 2 parts of aluminum stearate, add and stir until transparent, autoclave and set aside, that is the oil phase.

[0061] 2.1.2 Preparation of water phase Mix the two inactivated antigens in a sterile container at a ratio of 1:1, add 4% sterilized and cooled Tween-80, and stir while adding to make Tween-80 Until it is completely dissolved, it is the water phase.

[0062] 2.1.3 Emulsification The rotor of the emulsification machine is soaked and disinfected with 0.5% formaldehyde solution for more than 4 hours, and rinsed with hot sterile distilled water before use. Take 2 parts of the oil phase and put it in the emulsifier, start the motor to stir, then slowly add 1 part of the water phase, and then emulsify at 17500r...

Embodiment 3

[0065] The inspection of two kinds of virus single vaccines of embodiment 3 and double vaccine

[0066] 3.1 Properties, sterility test

[0067] The finished product appearance, dosage form and stability of each vaccine are all qualified, indicating that the traits of each vaccine are qualified;

[0068] Each vaccine was free of bacteria, mold and mycoplasma contamination, indicating that each vaccine passed the sterility test.

[0069] 3.2 Safety inspection

[0070] 40 healthy egg-laying shelducks tested negative for duck yellow virus antibody from non-epidemic areas were randomly divided into 4 groups after being fed for 1 week, with 10 ducks in each group, and the first group was injected intramuscularly with duck tambushu virus and duck plague Live vaccine, a single dose inoculation of 3.0mL / animal, the second group intramuscular injection of duck Tembusu virus inactivated vaccine, a single dose inoculation of 3.0mL / animal, the third group intramuscular injection of duck ...

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Abstract

The invention provides a vaccine composition. The vaccine composition comprises an immunization amount of duck tembusu virus antigen, an immunization amount of duck plague virus antigen and one or several adjuvants acceptable in veterinary medicine. The vaccine composition not only can simplify the immunization procedure achieving the purpose of preventing two diseases by injection once, but also can generate protective immunity against duck tembusu virus and duck plague and the phenomena of laying rate decrease or even zero laying rate and mass mortality for a duck group which result from mixed infection of the two diseases can also be avoided.

Description

technical field [0001] The invention relates to a vaccine composition and its preparation method and application. Background technique [0002] Duck Tembusu virus (Duck Tembusu virus) belongs to the Flavivirus genus of the family Flaviviridae. It mainly infects laying ducks, breeding ducks and geese. The main feature is death, with hemorrhagic oophoritis as the main lesion. Since 2010, the disease has mainly occurred in my country's Fujian, Shandong, Zhejiang, Shanghai, Jiangsu and other major duck breeding areas. [0003] Duck plague (Duck Plague), also known as duck viral enteritis, is an acute, contact, and septic infectious disease of waterfowl such as ducks, geese, and swans caused by duck plague virus in herpes virus. , body cavity hemorrhage, eruptive lesions of gastrointestinal mucosa, lymphoid organ lesions and degenerative changes of parenchymal organs are the main features. The OIE lists it as a category B disorder. The disease can infect ducks of any breed, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/245A61K39/12A61K9/107A61P31/14
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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