7 results about "Viral enteritis" patented technology

The invention relates to the field of pathogen detection, in particular to a primer probe group, a gene chip and a kit for detecting seven waterfowl infectious disease pathogens and application. The primer probe group provided by the invention can be used for simultaneously detecting the goose parvovirus, the duck viral enteritis virus, the muscovy duck parvovirus, the duck hepatitis A virus type 1, the duck hepatitis A virus type 3, the duck tembusu virus and the novel duck reovirus, and is strong in specificity and high in sensitivity. Furthermore, a gene chip prepared by using the primer probe group provided by the invention not only has strong specificity and high sensitivity (1.0 * 100 copies / [mu] L), but also has good repeatability and stability, can be stably stored within at least 180 days, has sensitivity superior to that of a standard fluorescent quantitative PCR method, can rapidly and efficiently detect main epidemic disease pathogens of waterfowl together, and has good application prospects. The method can be used for epidemiological investigation and disease diagnosis of waterfowls.

The present invention relates to the field of pathogen detection, in particular to a primer-probe set, gene chip, kit and application for detecting seven kinds of waterfowl infectious disease pathogens. The primer-probe set provided by the present invention can simultaneously detect goose parvovirus, duck viral enteritis virus, Muscovy duck parvovirus, duck hepatitis A virus type 1, duck hepatitis A virus type 3, duck Tembusu virus and novel duck reovirus, and has strong specificity and high sensitivity. Further, the gene chip prepared by using the primer-probe set provided by the present invention not only has strong specificity and high sensitivity (1.0×10<supgt;0< / supgt> copies / μL), but also has good repeatability and stability, can be stably stored for at least 180 days, and its sensitivity is also better than that of the standard fluorescence quantitative PCR method. It can quickly and efficiently co-detect the above-mentioned main pathogens of waterfowl diseases and can be used for the epidemiological investigation and disease diagnosis of waterfowl.

The present application relates to a kind of veterinary immunity-enhancing antiviral powder and its preparation method, the veterinary immunity-enhancing antiviral powder includes traditional Chinese medicine fermentation powder 60-70%, phoenix tree seed oil 20-30% and dioscorea opuloides mucopolysaccharide 10-20% according to mass percentage;The traditional Chinese medicine fermentation powder is first obtained fermentation product using bacillus velezensis, Candida utilis fermentation scutellaria baicalensis, astragalus, pomegranate peel, hawthorn and polygonatum sibiricum, then is formed by drying, ultrafine grinding to fermentation product.This application can improve the quality concentration of piglet serum immunoglobulin, so that piglet immunity, antiviral capacity is improved, in addition, it has good therapeutic effect on piglet viral gastroenteritis.

This invention relates to the use of berberine sulfate in the preparation of drugs for the prevention and / or treatment of viral enteritis. This invention is the first to propose the application of berberine sulfate in the treatment of viral intestinal inflammation, expanding the scope of application for this class of drugs. This invention has found that berberine sulfate can alleviate intestinal inflammation caused by papain-like proteins in animal models and may play a role in the treatment of intestinal inflammation caused by the novel coronavirus SARS-CoV-2.

This invention discloses a fusion protein expressing novel duck reovirus σC protein and duck-derived goose parvovirus VP1 protein, a recombinant duck viral enteritis virus, a vaccine, its construction method, and its applications, belonging to the field of recombinant virus technology. The nucleotide sequence of the fusion protein is shown in positions 300-3568 of SEQ ID NO:1. The fusion protein of this invention can simultaneously express protective antigen proteins of novel duck reovirus (NDRV) and duck-derived goose parvovirus (DGPV). Using CRISPR / Cas9 gene editing technology, the fusion protein is recombined into the US3 gene of duck viral enteritis virus. The resulting recombinant duck viral enteritis virus not only provides protection against virulent duck viral enteritis virus challenge but also provides good protection against NDRV and DGPV, achieving a multi-protection effect with a single injection.

The invention relates to application of coptisine sulfate in preparation of a medicine for preventing and / or treating viral enteritis. The application of coptisine sulfate in treatment of viral intestinal inflammation is provided for the first time, and the application range of the medicine is widened. It is found that coptisine sulfate can relieve intestinal inflammation caused by papain-like protein on an animal model, and can play a role in treatment of intestinal inflammation caused by novel coronavirus caused by SARS-CoV-2.

The invention discloses a fusion protein for expressing a novel duck reovirus sigma C protein and a duck-origin goose parvovirus VP1 protein, a recombinant duck virus enteritis virus, a vaccine, a construction method and application, and belongs to the technical field of recombinant viruses. The nucleotide sequence of the fusion protein disclosed by the invention is shown as the 300th to 3568th sites of SEQ ID NO: 1. The fusion protein can simultaneously express protective antigen proteins of a novel duck reovirus (NDRV) and a duck-origin goose parvovirus (DGPV), the fusion protein is recombined into a US3 gene of the duck viral enteritis virus by utilizing a CRISPR / Cas9 gene editing technology, and the obtained recombinant duck viral enteritis virus can provide protection for virulent attack of the duck viral enteritis virus, and can also provide a protective effect for the duck viral enteritis virus. And a good protection effect on the NDRV and the DGPV can also be achieved, and the one-needle multi-prevention effect is achieved.