11 results about "Duck plague virus" patented technology

The invention discloses a recombinant duck plague virus protein combination, a subunit vaccine as well as a preparation method and application of the subunit vaccine, and belongs to the technical field of vaccine preparation. According to the technical scheme, the recombinant duck plague virus protein combination comprises recombinant gE protein and recombinant gI protein; the amino acid sequence of the recombinant gE protein is as shown in SEQ ID NO: 10, and the amino acid sequence of the recombinant gI protein is as shown in SEQ ID NO: 13. The nucleotide sequence of the nucleic acid molecule for coding the recombinant gE protein is as shown in SEQ ID NO: 12; the nucleotide sequence of the nucleic acid molecule for coding the recombinant gI protein is as shown in SEQ ID NO: 15. The duck plague subunit vaccine provided by the invention adopts gE and gI co-expression, only contains part of antigens of DPV, does not contain genetic materials, does not have toxin expelling and dispersing risks, is good in safety, can enable the spatial conformation of gE protein to be close to that of natural protein by co-expression of gE and gI, can realize 10 / 10 protection of challenge, has the total protein expression amount of about 4000 [mu] g / ml, and can be used for preparing a vaccine for the duck plague. The culture medium can be diluted by 40 times for seedling production, so that the production cost is greatly reduced.

The application discloses a novel goose parvovirus VP1 protein truncated body gene, a recombinant duck plague virus and application. The novel goose parvovirus VP1 protein truncated body gene has a nucleotide sequence shown as SEQ ID No. 1. The recombinant duck plague virus has the novel goose parvovirus VP1 protein truncated body gene inserted into the genome of the recombinant duck plague virus. The application obtains a novel goose parvovirus VP1 protein truncated body gene, inserts the truncated body into the genome of the recombinant duck plague virus, and obtains the recombinant duck plague virus, and the rsVP1-Cre immune group shortens the NGPV discharge in the duck body, and the duck body immune rsVP1-Cre is helpful to the NGPV discharge. It is shown that the recombinant duck plague virus with the VP1 protein truncated body gene inserted can be used for preparation of a novel goose parvovirus-duck plague virus double live vector vaccine.

This invention belongs to the technical field of virus detection, specifically relating to a PCR detection kit for the simultaneous detection of four DNA viruses in waterfowl and its application. The PCR detection primer set for the simultaneous detection of four DNA viruses in waterfowl of this invention is the optimal primer set obtained through experimental screening. The kit composed of these primers can rapidly and specifically amplify and diagnose Muscovy duck parvovirus, duck plague virus, duck adenovirus type 3, and duck circovirus simultaneously, exhibiting high specificity and sensitivity. It can accurately and rapidly identify MDPV, DPV, DAdV-3, and DuCV, and can complete quantitative real-time PCR detection in approximately 50 minutes. The kit is simple and practical to operate, achieving efficient simultaneous detection of four viruses, shortening the detection cycle, increasing sensitivity by 10-1000 times, significantly improving early disease diagnosis capabilities, significantly reducing the misdiagnosis rate of diseases in poultry farms, and contributing to the sustainable development of the poultry farming industry.

The invention discloses a recombinant duck plague virus, which is characterized in that a duck egg yield reduction syndrome virus VP3 protein gene expression sequence is inserted into a short unique region of a duck plague virus attenuated strain genome, and the duck egg yield reduction syndrome virus VP3 protein gene expression sequence is a nucleotide sequence for encoding an amino acid sequence as shown in SEQ ID NO.1. The invention also discloses a preparation method and application of the recombinant duck plague virus. The recombinant duck plague virus disclosed by the invention can be used as a divalent vaccine candidate strain for simultaneously preventing duck egg yield reduction syndrome virus and duck plague virus, and has a wide application prospect.

The invention discloses a duck plague virus UL51 gene deletion strain and a construction method and application thereof, the deletion strain is preserved in China Center for Type Culture Collection in Wuhan University in China on April 2, 2025, and the preservation number is CCTCC NO: V202523. The method comprises the following steps: deleting a UL51 gene to obtain an infectious clone pDPV CHv-deltaUL51 of a UL51 gene deleted strain, and transfecting a duck source cell with the infectious clone DNA to obtain the UL51 gene deleted virus strain DPV CHv-deltaUL51. Detecting immune ducklings infected by viruses, and finding that the deletion of the UL51 gene weakens the duplication of DPV in the ducks and reduces the pathogenicity of the ducks; after the immunized duck is attacked by the DPV virulent CHv strain, the DPV virulent CHv strain can be prevented from lethal attack. The virus strain constructed by the invention has reduced pathogenicity to ducks, and has the potential of being used as a universal vector of an attenuated vaccine or a duck live vector vaccine.

This invention discloses an unconventional primer-probe set for detecting duck plague virus using the RAA fluorescence method, along with its applications and a kit, relating to the field of molecular biology. The unconventional primer-probe set of this invention includes primer pairs and probes. The primer pairs include a forward primer F1 and a reverse primer R3. The nucleotide sequence of the forward primer F1 is shown in SEQ ID NO.1, and the nucleotide sequence of the reverse primer R3 is shown in SEQ ID NO.2. The nucleotide sequence of the probe is shown in SEQ ID NO.3. Using the unconventional primer-probe set of this invention for the detection of duck plague virus enables rapid detection within 20 minutes and offers advantages such as ease of operation, low cost, high sensitivity, and strong specificity.

The invention belongs to the technical field of genetic engineering, and particularly relates to a recombinant duck plague virus for expressing H9 subtype avian influenza virus HA protein and application of the recombinant duck plague virus. The recombinant virus takes a duck plague virus attenuated strain VAC / delta TK, VAC / delta TK-delta gE / gI or VAC / delta TK-delta gE / gI-delta UL4 as a skeleton, and an H9 subtype avian influenza virus HA gene is inserted into a spacer region of a UL55 gene and an LORF11 gene. The nucleotide sequence of the H9 subtype avian influenza virus HA protein is shown as SEQ ID NO. 1 in a sequence table. The recombinant duck plague virus attenuated strain vector vaccine provided by the invention has good proliferation characteristic and hereditary stability, can induce an organism to generate effective humoral immune response after immunization, can be used as a general avian influenza vaccine for poultry such as chickens and ducks, and has a good application prospect.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an MDVgC promoter in construction of a recombinant herpes virus vector vaccine. The heterologous gene expressed by the MDVgC promoter is inserted into the herpes virus, especially turkey herpes virus, Marek's disease virus, porcine pseudorabies virus, bovine herpes virus, duck plague virus, human herpes virus (type 1-8), canine herpes virus or feline herpes virus, and the constructed recombinant herpes virus vector vaccine can efficiently express the heterologous gene and is safe and effective.

The invention belongs to the technical field of biology, and particularly relates to a duck plague virus vaccine strain with five simultaneously deleted genes as well as a construction method and application of the duck plague virus vaccine strain. The duck plague virus vaccine strain provided by the invention is a duck plague virus 3D strain, and the preservation number is CGMCC No.46648. Compared with duck plague virus CVCC AV1221, a UL56 gene, a LORF5 gene, a UL2 gene, a US7 gene and a US8 gene are deleted at the same time, the strain has no pathogenicity to ducks, has good immunogenicity and hereditary stability, can effectively resist duck plague virus infection, can resist 1000 MLD duck plague virulent virus attack only by immunizing 50 TCID50, and has good application prospects. The immune protection rate reaches 100%, and the vaccine has the potential of being used as an attenuated vaccine.

The application belongs to the technical field of biotechnology, and particularly relates to a duck plague virus vaccine strain with simultaneous deletion of five genes, a construction method thereof and application. The duck plague virus vaccine strain provided by the application is a duck plague virus 3D strain, and the preservation number is CGMCC No. 46648. UL56 gene, LORF5 gene, UL2 gene, US7 gene and US8 gene is simultaneously deleted, and the strain is non-pathogenic to ducks, has good immunogenicity and genetic stability, can effectively resist duck plague virus infection, and can resist 1000 MLD of duck plague virus strong virus attack only by immunizing 50 TCID 50 of the strain, and the immunoprotection rate reaches 100%, and the strain has potential as a live attenuated vaccine.