PCR detection kit for simultaneous detection of four DNA viruses of waterfowl and application thereof

By developing a multiplex real-time PCR detection method for four DNA viruses in waterfowl, the problem of rapid, sensitive, and specific detection of mixed infections of multiple pathogens in waterfowl farming has been solved, achieving efficient simultaneous detection and significantly improving detection efficiency and sensitivity.

CN122279102APending Publication Date: 2026-06-26ZHONGKAI UNIV OF AGRI & ENG
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHONGKAI UNIV OF AGRI & ENG
Filing Date
2026-04-03
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing technologies are insufficient for the rapid, sensitive, and specific simultaneous detection of mixed infections of duck circovirus, duck adenovirus, duck plague virus, and Muscovy duck parvovirus in waterfowl farming. Traditional methods are inefficient, costly, and cannot meet the needs of rapid clinical diagnosis and screening of large numbers of samples.

Method used

A multiplex quantitative PCR method for detecting four DNA viruses in waterfowl was developed. Using a specific PCR detection primer set and kit, it can simultaneously detect Muscovy duck parvovirus, duck plague virus, duck adenovirus type 3, and duck circovirus. Efficient and simultaneous detection is achieved through probes labeled with fluorescent reporter groups and quencher groups.

Benefits of technology

It enables efficient simultaneous detection of four pathogens, shortens the detection cycle to 1.4 hours, increases sensitivity by 10-1000 times, significantly improves the ability to diagnose diseases in the early stage, reduces the false positive rate, and helps the sustainable development of the poultry farming industry.

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Abstract

This invention belongs to the technical field of virus detection, specifically relating to a PCR detection kit for the simultaneous detection of four DNA viruses in waterfowl and its application. The PCR detection primer set for the simultaneous detection of four DNA viruses in waterfowl of this invention is the optimal primer set obtained through experimental screening. The kit composed of these primers can rapidly and specifically amplify and diagnose Muscovy duck parvovirus, duck plague virus, duck adenovirus type 3, and duck circovirus simultaneously, exhibiting high specificity and sensitivity. It can accurately and rapidly identify MDPV, DPV, DAdV-3, and DuCV, and can complete quantitative real-time PCR detection in approximately 50 minutes. The kit is simple and practical to operate, achieving efficient simultaneous detection of four viruses, shortening the detection cycle, increasing sensitivity by 10-1000 times, significantly improving early disease diagnosis capabilities, significantly reducing the misdiagnosis rate of diseases in poultry farms, and contributing to the sustainable development of the poultry farming industry.
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