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Vaccine vector preventing FAdV-4 and NDV and preparation method and application of vaccine vector

A vaccine carrier, fadv-4 technology, applied in the field of FAdV-4 and NDV prevention, to achieve good preventive effect, good vaccine immune protection effect, and simplified vaccine immunization procedures

Active Publication Date: 2019-11-22
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the combined prevention of Newcastle disease virus and FAdV-4 vaccine

Method used

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  • Vaccine vector preventing FAdV-4 and NDV and preparation method and application of vaccine vector
  • Vaccine vector preventing FAdV-4 and NDV and preparation method and application of vaccine vector
  • Vaccine vector preventing FAdV-4 and NDV and preparation method and application of vaccine vector

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Embodiment 1

[0045] The preparation method of the vaccine carrier that prevents FAdV-4 and NDV in the present embodiment may further comprise the steps:

[0046] In order to study the matching effect of the genes in FAdV-4 and the NDV LaSota vaccine strain, the vaccine vectors of Fiber 1 and Fiber 2 were simultaneously constructed in the present invention.

[0047] 1. Primer design

[0048] In order to construct the eukaryotic expression vectors of Fiber 1 and Fiber 2 of recombinant FAdV-4, this experiment obtained the genome sequence of FAdV-4 through genbank query, and selected the conserved sequence about 100 bp away from the target gene Fiber 1 and Fiber 2 to design primers ade-Fiber 1F, ade-Fiber 1R, ade-Fiber 2F and ade-Fiber 2R, so as to ensure that the target gene sequence is not subject to base mutation due to artificial primer design, and this fragment was named ade-Fiber 1 and ade-Fiber2 respectively. After ade-Fiber 1 and ade-Fiber 2 were successfully amplified, new primers fo...

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Abstract

The invention relates to a vaccine vector preventing the FAdV-4 and the NDV and a preparation method and application of the vaccine vector, and belongs to the technical field of prevention for the FAdV-4 and the NDV. According to vaccine vector and the preparation method and application of the vaccine vector, based on the structure characteristic of the FAdV-4, an FAdV-4 protective antigen Fiber 1protein is selected as a research and development object, and by constructing a recombinant Newcastle disease virus expressing the Fiber 1, a novel genetic engineering vaccine capable of preventing the FAdV-4 and the NDV is obtained through research and development; through research and development of the vaccine, not only is an important tool provided for prevention and control over the FAdV-4,but also a LaSota toxic strain of the Newcastle disease virus is selected as the vaccine vector, the toxic strain is a common vaccine toxic strain in production, an effect can be achieved that one strain prevents the two viruses, and therefore the vaccine immunization process is simplified.

Description

technical field [0001] The invention relates to a vaccine carrier for preventing FAdV-4 and NDV, a preparation method and application thereof, and belongs to the technical field of FAdV-4 and NDV prevention. Background technique [0002] Fowl Adenovirus (Fowl Adenovirus, FAdV) belongs to the family Adenoviridae and is a non-enveloped double-stranded DNA virus. Avian adenoviruses can be divided into three subgroups, including groups I, II and III, and group I of avian adenoviruses includes twelve serotypes, and different serotypes can infect chickens. Avian adenovirus serotype 4 (FAdV-4) is the causative agent of hydropericardium syndrome (HPS), hepatitis-hydropericardium syndrome (HHS) and inclusion body hepatitis (IBH), causing significant economic losses to the poultry industry. The disease caused by FAdV-4 affects broiler chickens at 3-6 weeks of age and is mainly characterized by hepatitis, nephritis and accumulation of clear or yellowish fluid in the pericardium of inf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/66A61K39/295A61K39/235A61K39/17A61P31/20A61P31/14
CPCC12N7/00C07K14/005C12N15/66A61K39/12A61P31/20A61P31/14C12N2760/18121C12N2760/18243C12N2710/10222A61K2039/70A61K2039/53C12N2710/10234C12N2760/18134
Inventor 何雷杨梓赵战勤王臣张春杰郁川李新新王海蓉
Owner HENAN UNIV OF SCI & TECH
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