Vaccine vector preventing FAdV-4 and NDV and preparation method and application of vaccine vector

A vaccine carrier, fadv-4 technology, applied in the field of FAdV-4 and NDV prevention, to achieve good preventive effect, good vaccine immune protection effect, and simplified vaccine immunization procedures

Active Publication Date: 2019-11-22
HENAN UNIV OF SCI & TECH
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the combined prevention of Newcastle disease virus and FAdV-4 vaccine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vaccine vector preventing FAdV-4 and NDV and preparation method and application of vaccine vector
  • Vaccine vector preventing FAdV-4 and NDV and preparation method and application of vaccine vector
  • Vaccine vector preventing FAdV-4 and NDV and preparation method and application of vaccine vector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation method of the vaccine carrier that prevents FAdV-4 and NDV in the present embodiment may further comprise the steps:

[0046] In order to study the matching effect of the genes in FAdV-4 and the NDV LaSota vaccine strain, the vaccine vectors of Fiber 1 and Fiber 2 were simultaneously constructed in the present invention.

[0047] 1. Primer design

[0048] In order to construct the eukaryotic expression vectors of Fiber 1 and Fiber 2 of recombinant FAdV-4, this experiment obtained the genome sequence of FAdV-4 through genbank query, and selected the conserved sequence about 100 bp away from the target gene Fiber 1 and Fiber 2 to design primers ade-Fiber 1F, ade-Fiber 1R, ade-Fiber 2F and ade-Fiber 2R, so as to ensure that the target gene sequence is not subject to base mutation due to artificial primer design, and this fragment was named ade-Fiber 1 and ade-Fiber2 respectively. After ade-Fiber 1 and ade-Fiber 2 were successfully amplified, new primers fo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a vaccine vector preventing the FAdV-4 and the NDV and a preparation method and application of the vaccine vector, and belongs to the technical field of prevention for the FAdV-4 and the NDV. According to vaccine vector and the preparation method and application of the vaccine vector, based on the structure characteristic of the FAdV-4, an FAdV-4 protective antigen Fiber 1protein is selected as a research and development object, and by constructing a recombinant Newcastle disease virus expressing the Fiber 1, a novel genetic engineering vaccine capable of preventing the FAdV-4 and the NDV is obtained through research and development; through research and development of the vaccine, not only is an important tool provided for prevention and control over the FAdV-4,but also a LaSota toxic strain of the Newcastle disease virus is selected as the vaccine vector, the toxic strain is a common vaccine toxic strain in production, an effect can be achieved that one strain prevents the two viruses, and therefore the vaccine immunization process is simplified.

Description

technical field [0001] The invention relates to a vaccine carrier for preventing FAdV-4 and NDV, a preparation method and application thereof, and belongs to the technical field of FAdV-4 and NDV prevention. Background technique [0002] Fowl Adenovirus (Fowl Adenovirus, FAdV) belongs to the family Adenoviridae and is a non-enveloped double-stranded DNA virus. Avian adenoviruses can be divided into three subgroups, including groups I, II and III, and group I of avian adenoviruses includes twelve serotypes, and different serotypes can infect chickens. Avian adenovirus serotype 4 (FAdV-4) is the causative agent of hydropericardium syndrome (HPS), hepatitis-hydropericardium syndrome (HHS) and inclusion body hepatitis (IBH), causing significant economic losses to the poultry industry. The disease caused by FAdV-4 affects broiler chickens at 3-6 weeks of age and is mainly characterized by hepatitis, nephritis and accumulation of clear or yellowish fluid in the pericardium of inf...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/66A61K39/295A61K39/235A61K39/17A61P31/20A61P31/14
CPCC12N7/00C07K14/005C12N15/66A61K39/12A61P31/20A61P31/14C12N2760/18121C12N2760/18243C12N2710/10222A61K2039/70A61K2039/53C12N2710/10234C12N2760/18134
Inventor 何雷杨梓赵战勤王臣张春杰郁川李新新王海蓉
Owner HENAN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap