Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Anti-porcine pseudorabies and swine flu vaccine composition and application thereof

A technology of vaccine composition and porcine pseudorabies virus, applied in antiviral agents, antibody medical components, medical preparations containing active ingredients, etc., can solve poor cross-protection, reduced vaccine immune effect, swine influenza virus hemagglutinin problems such as large differences

Active Publication Date: 2014-07-02
PU LIKE BIO ENG
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immunization is an important measure for the prevention and control of porcine pseudorabies and swine influenza, but there is no porcine pseudorabies, swine influenza dual vaccine or vaccine composition for sale on the market. The main reasons are: porcine pseudorabies virus can cause immunosuppression, and When other vaccines are used in combination, the immune effect of the vaccine can be reduced; in addition, the current swine influenza vaccine is an inactivated vaccine, while swine pseudorabies is a live virus vaccine, and the inactivating agents (such as formaldehyde, β-propane, etc.) in the inactivated vaccine Esters) and vaccine adjuvants (such as mineral oil adjuvants) will inactivate or interfere with live viruses, and will reduce the immune effect of vaccines. Therefore, swine pseudorabies and swine influenza are prepared into dual vaccines or vaccine compositions, It is necessary to solve the interference problem among inactivating agents, adjuvants, and antigens to achieve a definite immune effect
[0005] Chinese patent CN1624143A discloses a recombinant pseudorabies virus expressing the swine influenza virus hemagglutinin gene and its application. The recombinant virus only expresses the H3 subtype swine influenza virus hemagglutinin (Hemagglutinin, HA) gene, and other subtypes (such as the current The popular H1 subtype swine influenza) cannot provide effective protection, because the hemagglutinin of different subtypes of swine influenza virus is quite different, so the cross protection between different subtypes is poor, and there is a potential risk to animals when expressed by genetic engineering

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anti-porcine pseudorabies and swine flu vaccine composition and application thereof
  • Anti-porcine pseudorabies and swine flu vaccine composition and application thereof
  • Anti-porcine pseudorabies and swine flu vaccine composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: the preparation of anti-porcine pseudorabies, swine flu vaccine composition

[0054] 1. Preparation of porcine pseudorabies virus antigen

[0055] Spinner bottle cell culture method. The porcine pseudorabies virus gene deletion strain (SA215 strain, TK - / gE - / gI - Three-gene deletion, disclosed in Chinese patent CN101186902, the strain was deposited in CCTCC with the preservation number V200002) was properly diluted with virus diluent (serum-free DMEM medium), and inoculated on monolayer ST cells according to M.O.I=0.1 (purchased from CCTCC, No. GDC0060), gently rotate the cell flask for 2 weeks, adsorb at 37°C for 30min, add DMEM cell maintenance solution containing 3% (v / v) calf serum, and rotate at 37°C (10-12 rpm / hours) for cultivation. Observe 1 to 2 times a day, the cells grow well, culture at 37°C for 2 to 5 days, harvest the cells and cell fluid, freeze and thaw 3 times, filter the virus fluid with a hollow fiber filter column (pore size 1....

Embodiment 2

[0069] Embodiment 2: Anti-porcine pseudorabies prepared by different lyoprotectants, swine influenza vaccine composition preservation and transportation comparison

[0070] 1. Materials

[0071] The anti-porcine pseudorabies and swine influenza vaccine compositions prepared by the optimized heat-resistant lyoprotectant are selected from V4 and V5 vaccines in Example 1; prior art configuration (5% lactose containing V / V, 10% skimmed milk aqueous solution ) prepared anti-pseudorabies and swine influenza vaccine compositions are V6 and V7. The antigen components of V6 and V4 are the same, only the lyoprotectant is different; the antigen components of V7 and V5 are the same, only the lyoprotectant is different.

[0072] 2. Experimental design

[0073] Vaccine compositions (V4, V5, V6 and V7) prepared with different lyoprotectants were sampled and inspected 10 days after they were placed at 37°C to determine the content of porcine pseudorabies virus in the vaccine composition, an...

Embodiment 3

[0078] Embodiment 3: Comparison of the production of PR antibodies after piglets are immunized with anti-pseudorabies and swine influenza vaccine compositions

[0079] 1. Materials

[0080] The anti-pseudorabies and swine influenza vaccine composition selects the V4 and V5 vaccines in Example 1; the porcine pseudorabies single vaccine selects the V1 vaccine in Example 1.

[0081] 2. Design of animal experiments

[0082] Select 30 21-day-old piglets and divide them into 3 groups, 10 pigs / group. In the first group, each pig is injected with the neck intramuscular injection of porcine pseudorabies single vaccine V12.0ml (1 dose / head), and in the second and third groups Each pig was intramuscularly injected with anti-porcine pseudorabies and swine influenza vaccine composition V4 and V5 2.0ml (1 portion / head). Twenty-eight days after immunization, the serum of piglets in each group was collected, and the antibody titer of porcine pseudorabies virus was determined by ELISA method...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an anti-porcine pseudorabies (PRV) and swine flu (SIV) vaccine composition. Besides porcine pseudorabies and swine flu, the composition can additionally prevent clinical manifestations of porcine respiratory disease complex (PRDC) caused by infection of other pathogenic causes. Furthermore, the invention provides application of the vaccine composition for preparing a medicine in preventing and / or controlling immunized pig PRDC disease. The composition not only avoids interference between antigens in a process of using but also achieves a surprising preventive effect on the porcine respiratory disease complex. Therefore, the vaccine composition disclosed by the invention can simplify an immune procedure and achieve an effect of preventing two diseases by one dosage. The anti-porcine pseudorabies and swine flu vaccine composition, after immunizing pigs, can effectively prevent spread of the PRDC.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry biopharmaceuticals, and relates to a vaccine composition prepared by using porcine pseudorabies live virus antigen and swine influenza virus inactivated antigen and application thereof. Background technique [0002] Pseudorabies (PR) is an infectious disease shared by a variety of animals, causing acute and fatal infectious diseases in livestock and wild animals, with fever, itching and encephalomyelitis as the main symptoms, and pigs are its natural susceptible hosts , mainly cause abortion, stillbirth and mummified fetuses in pregnant sows, piglet infection is mainly neurological symptoms and acute death, adult pigs are mainly respiratory tract infection, this disease has become the main cause of reproductive failure syndrome and a large number of piglet deaths. [0003] Swine influenza (SI) is an important respiratory disease that endangers the pig industry at present. It is mainly cha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/245A61K39/145A61K39/295A61P31/22A61P31/16
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com