7results about How to "Epidemic prevention" patented technology

The present invention discloses a G9P[23] genotype porcine rotavirus strain and its application; the G9P[23] genotype porcine rotavirus is isolated and passaged from a piglet diarrhea sample in a pig farm in Guangdong Province, named RVA / pig / CH / U945 / 2022 / G9P[23], and the deposit number is CCTCC NO: V202454. The isolated strain can stably proliferate in MA104 cells and produce typical cytopathic effects. The G9P[23] genotype porcine rotavirus strain isolated by the present invention can be used for the preparation of virus strains for testing and inactivated vaccines. As a vaccine strain, it has good immunogenicity and can produce a high level of antibodies after immunizing animals, providing the necessary material and theoretical basis for the subsequent research and development of rotavirus vaccines.

The invention discloses a primer group, a kit and a method for simultaneously detecting spike protein, envelope protein and transmembrane protein of bovine coronavirus. Belongs to the technical field of molecular biological detection. According to the method, simultaneous detection of the spike protein, the envelope protein and the transmembrane protein of the bovine coronavirus is realized, and compared with the prior art, the sensitivity, the specificity and the repeatability of detection are greatly improved, so that the method has important significance in preventing and controlling prevalence of the bovine coronavirus.

The application provides a kind of in vitro destruction of Helicobacter pylori biofilm by organosilicon quaternary ammonium salt, and relates to the technical field of anti-infective drugs and digestive system diseases prevention and treatment.A kind of in vitro destruction of Helicobacter pylori biofilm by organosilicon quaternary ammonium salt, comprising an aqueous solution of octadecyltrimethylsilane organosilicon quaternary ammonium salt OSA as an in vitro biofilm destruction working solution: the OSA molecule is composed of four parts: trimethoxysilane-linking segment, positively charged quaternary ammonium cation functional core, hydrophobic long alkyl and chloride ion counter ion; the working solution is prepared by uniformly mixing 1.5% OSA stock solution and 18.2 MΩ·cm sterile ultrapure water.The triple physical action combined with molecular pathway regulation efficiently destroys Helicobacter pylori biofilm, and the effect is significantly better than that of the standard triple regimen, has no drug resistance risk, has little disturbance to gastrointestinal flora, has a supporting standardized system, and can provide reliable support for Hp in vitro drug sensitivity and drug screening.

This invention discloses a method for year-round high-efficiency trellis seedling cultivation and propagation of strawberries. The steps are as follows: S1, constructing cultivation trellises; S2, transplanting mother seedlings from February to mid-March; S3, adjusting the cultivation trellis to a "high" position, placing the bagged mother seedlings on the cultivation trellis, and then managing the seedlings to promote strong growth; S4, from early July to early August, cutting off the runners, retaining 3-4 leaves and 1 bud for cutting propagation; S5, after the cutting propagation is completed, adjusting the cultivation trellis to a "low" position, removing the substrate soil and mother seedlings from the original planting bags, inserting them into new planting bags, filling them with substrate soil, and then placing the mother seedlings on the cultivation trellis; S6, managing the mother seedlings to promote strong growth; S7, flowering and fruiting begin in mid-to-late October. This method is simple, easy to operate, and has low facility investment costs. It is not only suitable for rapid propagation of mother seedlings on "high shelves" in spring and summer and meets the needs of soilless cultivation on "low shelves" in autumn and winter, but also greatly improves facility utilization and increases production efficiency.

The utility model discloses a dendrobe planting rain-proof shed device which comprises a plurality of stand columns and a dendrobe seedbed, the stand columns are arranged in two rows, one side of each row of stand columns is fixedly connected with a cross beam, a first steel rope is fixed between every two opposite stand columns, a plurality of pulleys are clamped on the first steel ropes in a sliding mode, the bottoms of the pulleys are fixedly connected with connecting rings, and the connecting rings are connected with the connecting rings in a sliding mode. First buckles are connected to the bottom ends of the connecting rings in a clamped mode, the same double-layer plastic film is connected between the multiple first buckles in a clamped mode, and multiple second steel ropes are fixed between the two cross beams. The double-layer plastic film is automatically shrunk manually, the dendrobium planting environment is sufficient in illumination, organic salt is reasonably utilized, the temperature and humidity are reasonably controlled in high-temperature and high-humidity seasons, ventilation and light transmission in the greenhouse are improved, the incidence rate of diseases and pests is reduced by 15-20%, the intensive dendrobium planting environment and the management level are remarkably improved, the canopy does not need to be cleaned manually, and the cost is reduced. And the labor management cost is reduced.

The present application discloses a passing type epidemic prevention device which can sterilize the body and personal belongings by being arranged in tourist passage facilities such as airports, ports and passenger stations, and multipurpose facilities such as shopping centers, supermarkets, exhibition halls and concert venues. The passing type epidemic prevention device comprises a first sterilization part for irradiating low-temperature plasma and ultraviolet rays, a second sterilization part for spraying and inhaling air, a third sterilization part for irradiating high-temperature plasma and ultraviolet rays, a thermal imaging camera for measuring the heat emission of a person, and a measurement part for outputting the heat emission of the person measured by the thermal imaging camera.

The present invention relates to a bovine viral diarrhea virus BVDV-SDJN01 and its application, and belongs to the field of bovine viral diarrhea virus vaccine reagents. The virus is the bovine diarrhea virus BVDV-SDJN01, which was sent to the China Center for Type Culture Collection on June 30, 2022 for preservation, and the preservation number is: CCTCC NO: V202255. The BVDV1c inactivated vaccine is prepared using the virus as an antigen, which has good immunogenicity and can stimulate the body to produce a high level of neutralizing antibodies. The virus strain provides an effective vaccine candidate strain for the effective prevention and treatment of bovine viral diarrhea in my country.