109results about How to "Epidemic prevention" patented technology

The invention discloses a porcine seneca valley virus strain and an application thereof. In the invention, the seneca valley virus strain CH-FJZZ-2017 is isolated from pathological materials of porcine idiopathic vesicular disease in a pig farm in Fujian Province, with the preservation number of CGMCC No. 12160. The isolated porcine seneca valley virus CH-FJZZ-2017 in the invention is isolated from swinery newly suffering from the epidemic disease within Chinese territory, represents the current epidemic predominant virus strain in China, has a good virus strain background, and can be used as an inactivated vaccine production virus strain and a virus seed for testing, thereby providing a material for subsequent relevant experimental study, and laying a material foundation.

The invention discloses an on-site detection immuno-chip, comprising a chip carrier and an agarose gel layer which is paved on the chip carrier, wherein, the agarose gel layer is fixed with a plurality of antibody microarrays of 4*4, and chip-dedicated fences or Super PAP Pen scribings are used for separating the microarrays from each other; the double antibody sandwich principle is adopted to detect the antigen to carry out the dot matrix of corresponding capture antibody, positive control and negative control on the solid phase carrier simultaneously; the antibody protein is connected with the solid phase carrier through the covalent bond and physical adsorption; the sample liquid to be detected and the chip are directly incubated; the antigen to be detected in the samples combine with the corresponding antibody which is fixed on the chip; a specific monoclonal antibody probe marked by horse radish peroxidase is added and the macroscopic detection results can be obtained after the coloration of substrates. The invention can detect viruses in multiple aquatic animal samples and the results are macroscopic, thus being applicable to rapid and accurate detection of viruses of aquatic animals in breeding production.

The invention relates to a porcine epidemic diarrhea virus strain MY01 and application thereof and belongs to the field of porcine epidemic diarrhea virus vaccine reagents. The porcine epidemic diarrhea virus strain MY01 is collected in China General Microbiological Culture Collection Center under CGMCC No. 11495. The porcine epidemic diarrhea virus strain MY01 is higher in pathogenicity for pigs and good in immunogenicity, a deactivated oil emulsion vaccine prepared using the strain is safe and reliable, homologous attacking protection can be provided, protection is also provided for PEDV (porcine epidemic diarrhea virus) epidemic strains, higher immunity can be generated after immunization, inoculated pigs are significantly lower in morbidity and mortality, the immunization effect is superior than that of existing commercial vaccines in the market, and the strain has the advantage of competing with like products home and abroad, can effectively prevent the prevalence and spread of porcine epidemic diarrhea virus and reduce economic cost caused by the disease and has a promising application prospect.

The invention relates to the field of vaccines of Haemophilus parasuis (HPs) in veterinary biological products and discloses a serotype 5 HPs vaccine strain. The class name of the vaccine strain is HPs. The strain number is XX0306. The vaccine strain has been collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:M2013095 and collection date being March 21, 2013. The serotype 5 HPs strain XX0306 has stronger pathogenicity toward swine and has good immunogenicity. The inactivated vaccines prepared from the vaccine strain are safe and reliable and have quite good protective effects on the swine challenging homological strains. The morbidity and mortality of the immunized swinery are obviously reduced. Either the single vaccine or the combined vaccine has the immune effects equivalent or superior to the immune effects of the existing commercialized vaccines and can effectively prevent prevalence of HPs.

In a thin client system in which clients are connected to remote machines via a network so as to implement transactions, a remote machine infected with a virus is isolated from the network in response to a user's instruction on each client whilst communication settings minimally required for transactions are maintained. That is, a request issue agent issues an isolation request in response to a user input, so that a request execute agent changes communication settings of the remote machine in response to the isolation request. In an isolated state of a remote machine isolated from the network, a management server is allowed to change network settings regarding the remote machine with reference to a disconnection setting file, which stores communication settings minimally required for the remote machine in advance.

The invention relates to the field of vaccines of Haemophilus parasuis (HPs) in veterinary biological products and discloses a serotype 4 HPs vaccine strain. The class name of the vaccine strain is HPs. The strain number is FS0307. The vaccine strain has been collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:M2013094 and collection date being March 21, 2013. The serotype 4 HPs strain FS0307 has stronger pathogenicity toward swine and has good immunogenicity. The inactivated vaccines prepared from the vaccine strain are safe and reliable and have quite good protective effects on the swine challenging homological strains. The morbidity and mortality of the immunized swinery are obviously reduced. Either the single vaccine or the combined vaccine has the immune effects equivalent or superior to the immune effects of the existing commercialized vaccines and can effectively prevent prevalence of HPs.

The invention discloses sterilizing and disinfecting traditional Chinese medicine incense. The sterilizing and disinfecting traditional Chinese medicine incense comprises the following components in percentage by weight: 65%-74% of rhizoma atractylodis, 25%-34% of plant glue powder and 1%-3% of a combustion improver. The invention also discloses a preparation method of the sterilizing and disinfecting traditional Chinese medicine incense. The traditional Chinese medicine incense prepared by the method has the advantages of good sterilizing and disinfecting effect, long hold time, convenience in use, low cost and no toxicity to human bodies.

The present invention is compound interferon inducing agent lozenge, relates to biomedicine engineering, and aims at providing lozenge capable of inducing body to generate endogenous interferon and with high curative effect, less side effect, small dosage and wide application. Each of the compound interferon inducing agent lozenge contains attenuated Newcastle disease virus vaccine or deactivated Newcastle disease virus vaccine 10-120 hemagglutination units, astragalus root 1-2000 mg, liquiritigenin 1-1000 mg, supplementary material and stabilizer. The compound interferon inducing agent lozenge is suitable for preventing SARS and other viral infectious respiratory tract diseases, and has also auxiliary treatment effect on various kinds of tumor, hepatitis B and hepatitis C.

The invention discloses a disease pre-warning system for a stichopus japonicus. The disease pre-warning system is used for monitoring main factors (such as temperature, salinity, pH, dissolved oxygen, chemical oxygen consumption, pathogen number change) in a stichopus japonicus cultivation environment; applying a disease pre-warning model to analyze monitoring data; pre-warming a disease generation probability of the stichopus japonicus and putting forward prevention and control measures; and releasing pre-warming information through mobile phone messages or a network platform, wherein a disease pre-warming model screens out main environmental factors which remarkably affect growth and healthy cultivation of the stichopus japonicus after analyzing and researching long-term monitoring data of the stichopus japonicus cultivation environment; through an orthogonality simulation experiment, influence of each main factor to a death rate is analyzed in a mode that a telophase death rate of the stichopus japonicus is used as indexes while an experiment is ended; and based on a great number of data obtained from the experiment, an established pre-warming model based on a SQL Server (Structured Query Language Server) relationship databank engine comprises prediction methods, such as a regression analysis, a relevance regulation analysis, an expertise analysis, a rational analysis on storage, a neural network analysis.

The invention provides a chewing medicine composition for preventing and treating mouth, nose and throat upper respiratory infecting and application thereof. The medicine composition comprises effective constituents used for preventing and/or treating mouth, nose and throat upper respiratory infecting symptoms or early-stage mouth, nose and throat upper respiratory infecting complication signs, and acceptable accessories of medicines; the medicine composition further comprises auxiliary constituents; the effective constituents used for preventing and/or treating the mouth, nose and throat upper respiratory infecting symptoms or the early-stage mouth, nose and throat upper respiratory infecting complication signs comprise medicine constituents which are not suitable for direct containing and direct chewing; and the proportion of the acceptable accessories of the medicines to the chewing medicine composition is larger than the proportion of accessories to traditional swallowing medicines used for preventing and/or treating mouth, nose and throat upper respiratory infecting. By means of the technology of the chewing medicine composition, the medicines which are unacceptable, not suitable for direct chewing or forbidden in chewing are prepared into a safely-contained efficient medicine preparation welcomed by patients; and the chewing medicine composition has the obvious effect on preventing and treating the mouth, nose and throat upper respiratory infecting.

The invention belongs to the technical fields of veterinary microbiology and zoonotic diseases, and in particular relates to application of immunogenic protein genes of streptococcus suis type-2 for separating, cloning and expressing exocellular peptides and recombinant proteins of the encoded proteins in vaccine and diagnosis. In the invention, a new immunogenic protein gene hp0245 is separated from SC-19 bacterial strains of streptococcus suis type-2 virulent strains, wherein DNA (hp0245EC) for encoding the exocellular peptides has nucleotide sequences represented in the sequence table SEQ ID No:1 and is encoded with 261 amino acids. The recombinant protein HP0245EC remains the immunogenic feature of the primitive protein, can provide effective immunological protection to the SC-19 bacterial strain of the streptococcus suis type-2 infected in mice and has potential application value of vaccine. The invention further comprises a composition and preparation method for a streptococcus suis type-2 HP0245-ELISA diagnostic reagent kit, and a preparation of hp0245EC for cloning hp0245EC escherichia coli; the recombinant escherichia coli has been preserved in the China Center for Type Culture Collection (CCTCC) and the preservation number is CCTCC No: M2010258.

The invention relates to the field of vaccines of Haemophilus parasuis (HPs) in veterinary biological products and discloses application of a serotype 5 HPs vaccine strain. The class name of the vaccine strain is HPs. The strain number is XX0306. The vaccine strain has been collected in the China Center for Type Culture Collection, with collection number being CCTCC No:M2013095 and collection date being March 21, 2013. The serotype 5 HPs strain XX0306 has stronger pathogenicity toward swine and has good immunogenicity. The inactivated vaccines prepared from the vaccine strain are safe and reliable and have quite good protective effects on the swine challenging homological strains. The morbidity and mortality of the immunized swinery are obviously reduced. Either the single vaccine or the combined vaccine has the immune effects equivalent or superior to the immune effects of the existing commercialized vaccines and can effectively prevent prevalence of HPs.

The invention relates to a method of make single valve RT-PCR dectecting masculine comparing virulence haemorrhagic septicemia virus, fish infectivity hemocytopoietic organ putrescence and infectivity pancreas putrescence germina. Its feature is selecting G gene from virulence haemorrhagic septicemia virus, VP gene from fish infectivity hemocytopoietic organ putrescence and N gene from infectivity pancreas putrescence germina, inserting into pUC57 particle carrier and transfect to DH5 alpha coliform bacteria to take in vitro expression, and extending the section 625bp, 371bp, 206bp. It has very high use value.

The invention discloses a chicken infectious bursal disease very virulent cell adapted strain and application thereof in preparation of a drug for preventing chicken infectious bursal disease. The cell adapted strain is the cell adapted strain of a chicken infectious bursal disease very virulent Gx strain, is namely as IBDV-G strain with culture collection number of CGMCC (China General Microbiological Culture Collection Center) 5553. The IBDV-G strain is inactivated and prepared into a vaccine to be used for animal immunization. The results prove that the vaccine is safe, and has 100% protective rate on very vilurent IBDV-G lethal hit; and the antibody positive rate, antibody titer and very virulent protective rate of the very virulent inactivated vaccine are ranked at the top as compared with those of the compared vaccines. Therefore, the popularization and application of the chicken infectious bursal disease very virulent cell adapted strain can effectively prevent the outbreak andprevalence of vvIBD in local area in China and favorably promote sustained, stable and sound development of immune areas or even poultry husbandry in China.

The invention provides detection primers and a detection method for quaternary ammonium compound agent (QacA) gene carrying condition in methicillin-resistant staphylococcus aureus (MRSA). The detection primers for the QacA gene carrying condition in the MRSA are characterized by comprising a QacA gene forward primer: 5'-ggttgtggaagaactttctcctt-3'; and a QacA gene reverse primer: 5'-ccaattccggaaggtaacac-3'. The detection method comprises the following steps of: taking a clinical sample separation strain which is positive in the MRSA by identification after culture, performing high-temperatureinactivation, and extracting sample DNA serving as a detection sample; taking a staphylococcus aureus strain without carrying QacA genes, performing high-temperature inactivation, and extracting sample DNA serving as a negative control; artificially synthesizing a QacA sequence, and constructing recombinant plasmids carrying QacA genes to obtain a positive control; and performing polymerase chainreaction (PCR). The detection primers and the detection method have the advantages of high detection rate and strong repeatability.

The invention discloses a fish lymphocystis disease virus (LCDV) immunity detection chip, and the structure of the chip comprises a chip carrier, an agarose gel layer covered on the chip carrier and a plurality of antibody microarrays fixed on the agarose gel layer; wherein, special fences for chips or Super PAP Pen rulings are arranged between the microarrays for separating the microarrays. In the invention, a sandwich method is adopted to detect antigen, pathogenic antibody is fixed on a film support of the chip, the to-be-detected sample solution is prepared by using virus target organ of a sample to be detected, the to-be-detected sample solution and the chip fixed with pathogenic antibody are directly incubated, a specific monoclonal antibody (McAb) probe with a fluorescent mark is added, and then a CCD scanner is used for reading the result. The invention has the advantages of detecting lymphocystis disease virus in various samples or different tissues in the same sample simultaneously, which can be used for rapid, sensitive, and accurate detection on LCDV in marine-culturing fishes and quarantine on LCDV in imported-exported fishes.

The invention provides a genetic engineering subunit oral bicombined inactivated vaccine, and in particular relates to a porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus genetic engineering subunit oral bicombined inactivated vaccine, which is prepared by using lactococcus lactis capable of carrying out recombinant expression on S protein of porcine epidemic diarrhea virus and SLN protein of porcine transmissible gastroenteritis virus, wherein an amino acid sequence of the S protein of the porcine epidemic diarrhea virus is SEQ ID No. 1, a nucleotide sequence of a coding gene of the S protein of the porcine epidemic diarrhea virus is SEQ ID No. 2, and the SLN protein is formed by connecting genes of A and D antigenic sites with a gene of an N321 antigenic site of the porcine transmissible gastroenteritis virus. The prepared inactivated vaccine can provide effective homologous attacking protection; after being used for immunization, the inactivated vaccine can generate stronger immunity; after a pig group is inoculated with the vaccine, the morbidity and mortality rates of the pig group are obviously reduced; the genetic engineering subunit oral bicombined inactivated vaccine can be used for effectively preventing the prevalence and spread of porcine epidemic diarrhea and porcine transmissible gastroenteritis and reducing the economic loss, caused by the diseases, of pig husbandry, thus having wide application prospect.

The invention discloses an application of a tulathromycin composite in treating or preventing pet bacterial diseases, and also discloses a tulathromycin composite used for treating or preventing pet bacterial diseases and a preparation method thereof. The composite disclosed by the invention has the characteristics of less dosage and good curative effect, and is used for preparing medicament widely used clinically.

The invention discloses a high-throughput micro-fluidic LAMP chip for detecting livestock respiratory tract pathogens and a detection method, and belongs to the technical field of biochips. The LAMP chip comprises an LAMP amplification reagent, a DNA template, an LAMP quality control positive control, an LAMP quality control negative control, a detection chip, a chip probe and a microfluidic LAMPchip analyzer, wherein the detection chip is arranged in the isothermal amplification microfluidic LAMP chip analyzer; and the chip probe comprises nucleotide sequences as shown in SEQ ID NO: 1-23. The LAMP chip is used for detecting specific nucleic acid fragments of mannheimia haemolytica, pasteurella multocida, mycoplasma, arcanobacterium pyogenes and klebsiella pneumoniae. The LAMP chip provided by the invention can complete detection within 50 minutes, has a detection limit of 1.5x10<-2> ng/mu L, can monitor main livestock respiratory tract pathogens in real time, has the characteristicsof simple operation, rapid detection, high sensitivity and strong specificity, and brings convenience to detection of the livestock respiratory tract pathogens.

The invention discloses a high-flux micro-fluidic chip for detecting pathogeny of the epidemic disease of the respiratory tract of pigs and a detection method and belongs to the technical field of biological chips. The high-flux micro-fluidic chip comprises an LAMP amplification reagent, a DNA/cDNA template, ddH2O, LAMP positive control, LAMP negative control, a detection chip, a chip probe and amicro-fluidic chip analyzer, the detection chip is placed in the constant-temperature amplification micro-fluidic chip analyzer, and the chip probe comprises nucleotide sequences shown in SEQ ID NO:1-42 and specific nucleic acid segments applied to detection of the hog cholera virus, porcine reproductive and respiratory syndrome virus, porcine circovirus 2 type, porcine circovirus 3 type, haemophilus parasuis, mycoplasma hyopneumoniae, porcine pasteurella and swine influenza virus. According to the high-flux micro-fluidic chip, detection can be completed within one hour, the detection limit is5*10-2ng/microliter, different kinds of pathogeny of the epidemic disease are accurately distinguished, and the high-flux micro-fluidic chip has the advantages of quick detection, high sensitivity and accurate specificity.