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Mycoplasma hyorhinis strain, vaccine composition, preparation method and application thereof

A technology of Mycoplasma hyorrhea and vaccine composition, which is applied in the field of veterinary vaccines, can solve the problems of difficulty in screening vaccine strains, differences in the pathogenicity of strains, etc., and achieve the effect of improving the prevention and control effect.

Active Publication Date: 2014-12-31
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no vaccine product on the market for the prevention of this pathogen. In addition, the pathogenicity of the strains isolated from different places is significantly different, and it is extremely difficult to screen suitable vaccine strains.

Method used

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  • Mycoplasma hyorhinis strain, vaccine composition, preparation method and application thereof
  • Mycoplasma hyorhinis strain, vaccine composition, preparation method and application thereof
  • Mycoplasma hyorhinis strain, vaccine composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, the preparation of Mycoplasma hyorhina vaccine composition

[0052] 1. The source of bacteria (virus) strains

[0053] Mycoplasma belongs to Mycoplasma hyorhina LYH strain, which was isolated by our laboratory and preserved in the China Center for Type Culture Collection (CCTCC), address: Wuhan University, Wuhan, China, the preservation number is CCTCC NO: V201334, and the preservation date is August 20, 2013.

[0054] 2. Preparation and inspection of semi-finished products of Mycoplasma hyorhina vaccine

[0055] 2.1 Preparation of seeds for production

[0056] Inoculate the liquid culture medium with 10% freeze-dried strain of Mycoplasma hyorhinis LYH strain, culture at 37°C for 1-3 days, and harvest the bacterial liquid as first-class seeds when the color of the culture medium turns yellow and the pH value drops to 6.8-7.0.

[0057] Inoculate the first-grade seeds with 5% liquid medium, and culture at 37°C for 1-3 days. When the color of the medium tur...

Embodiment 2

[0080] The pathogenicity test of embodiment 2 Mycoplasma hyorhinois LYH strain infection

[0081] In this experiment, the pathogenesis model of artificially infected Mycoplasma hyorhina LYH strain was established, so as to prove that Mycoplasma hyorhina LYH strain can cause the occurrence of swine enzootic pneumonia (SEP).

[0082] 1 Material:

[0083] Animals: 8 to 9 weeks old, pigs with negative serum antibodies to Mycoplasma hyopneumoniae and Mycoplasma hyorhinosus

[0084] Attacking strain: Mycoplasma hyorhinosum LYH strain

[0085] 2 methods:

[0086]10 piglets aged 60-70 days were selected and randomly divided into 2 groups with 5 pigs in each group. Pigs in group 1 were challenged with LYH strain of Mycoplasma hyorhinois, and pigs in group 2 were not challenged as control. After 28 days of observation, the lungs were dissected, and the SEP pneumonia lesions of the experimental pigs were scored according to the 28-point method of Madec and Kobisch (1982). See Table ...

Embodiment 3

[0096] Efficacy test of the Mycoplasma hyorhina vaccine composition of embodiment 3 different antigen content

[0097] The purpose of this test is to evaluate the efficacy of the Mycoplasma hyorhina vaccine composition with different antigen contents against pathogenic Mycoplasma hyorhinophysis infection.

[0098] 1 material

[0099] The vaccine L1 (Mycoplasma hyorhinois LYH strain antigen content of the preparation of embodiment 1 is 10 8 CCU / ml) and vaccine H1 (Mycoplasma hyorhinosus LYH strain antigen content is 10 10 CCU / ml).

[0100] Animals: piglets aged 2 to 3 weeks, with negative serum antibodies to Mycoplasma hyopneumoniae and Mycoplasma hyorhinosus

[0101] Attacking strain: Mycoplasma hyorhinosum LYH strain

[0102] 2 methods:

[0103] Fifteen piglets aged 2-3 weeks were selected and randomly divided into 3 groups with 5 piglets in each group. On the 0th day, inject vaccine L1 into the neck muscle of each pig in the first group, and inject vaccine H1 into the ...

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Abstract

The invention provides a Mycoplasma hyorhinis strain LYH, and a vaccine composition prepared from the Mycoplasma hyorhinis strain LYH, in particular to a vaccine composition comprising the Mycoplasma hyorhinis and Mycoplasma hyopneumoniae. The vaccine composition can be effective in prevention and treatment of swine enzootic pneumonia caused by Mycoplasma hyorhinis, Mycoplasma hyopneumoniae single infection or mixed infection. Especially in the circumstance of mixed infection, immune effect of the vaccine composition significantly exceeds that of each single vaccine.

Description

technical field [0001] The invention relates to a new strain of mycoplasma hyorhina, a vaccine composition, a preparation method and application thereof, and belongs to the field of veterinary vaccines. Background technique [0002] Mycoplasma hyorhinis (Mhr) is a common parasitic pathogen in the upper respiratory tract of piglets (detected by Ross and Young in 1993). It has a high detection rate in pigs and is not pathogenic alone. Switzer isolated the Mycoplasma in 1953, 1954, and 1955, and officially named it Mycoplasma hyorhina (Mhr). Mycoplasma hyorhina (Mhr) infection is mainly transmitted through the respiratory tract, usually from sows or older pigs to piglets. Like Mycoplasma hyopneumoniae (Mhp), Mycoplasma hyopneumoniae can adhere to the ciliated epithelial cells of the respiratory tract, destroy the ciliated barrier and cause secondary infection of other bacteria, causing swine pneumonia, polyserositis, arthritis, and eustachian tube inflammation , otitis, conju...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/116A61K39/02A61P31/04C12R1/35
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
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