110 results about "Mycoplasmata" patented technology

The invention relates to bifidobacterium breve CCFM1025, a fermented food and application thereof. The bifidobacterium breve CCFM1025 can be used for improving the depression-like behavior of a depression mouse, improving 5-hydroxytryptamine in the brain of the depression mouse, improving the level of 5-hydroxytryptamine and a brain-derived neurotrophic factor, reducing the level of corticosteronein serum of the depression mouse, improving the level of 5-hydroxytryptamine in the serum of the depression mouse, improving intestinal flora disturbance of the depression mouse, reducing the abundance of intestinal veillonella, improving the abundance of bifidobacterium and mycoplasmataceae, improving alpha-diversity of intestinal flora and reducing occurrence of inflammatory bowel disease and obesity. By adopting the bifidobacterium breve CCFM1025, the mRNA level of tryptophan hydroxylase in a simulated entero-chromaffin cell can be improved, the secretion volume of 5-hydroxytryptophane ofthe cell can be improved, and a precursor substance is specifically provided to synthesis of 5-hydroxytryptamine in the brain. The bifidobacterium breve CCFM1025 has a wide application prospect.

The invention relates to the diagnostic medicine of animals, especially relates to a mycoplasma bovis diagnosis technology, and concretely relates to a multi-epitope fusion antigen having an amino acid sequence represented by SEQ ID NO:1 or SEQ ID NO:2, and its application in the preparation of a mycoplasma bovis diagnosis reagent. The diagnosis reagent can be used as a solid phase vector coating antigen of an indirect ELISA kit and is combined with its specific antibody, a horseradish peroxidase coupled anti-cattle IgG antibody is added and incubated, and a color development reaction is carried out, and the color development degree is proportional to the amount of the anti-mycoplasma bovis antibody in a sample to be measured. The technology has the advantages of simple operation, no need of complex equipment, low technical requirements on the laboratorial conditions and experiment personals, low detection cost, and suitableness for the large-scale development in the basic level and the culture farm; the multi-epitope fusion antigen has a low making cost and is suitable for large-scale application; and has the advantages of high sensitivity and specificity, small batch difference, and high detection result consistence because of the adoption of multi-epitope as a target.

The subject invention provides a plant cell culture for producing proteinaceous agents comprising a plant cell line stably transformed to express a transgene encoding a proteinaceous agent and a growth medium which supports the growth of said plant cell culture but which does not support the growth of Mycoplasmataceae and contains no materials of animal origin. The plant cell line is capable of being continuously passaged such that consistent transgene expression is maintained during passaging. The plant cell line is also capable of being cryopreserved such that consistent transgene expression is recovered upon recovery from cryopreservation.

The invention discloses a Mycoplasma bovis Mbov_0570 gene mutant strain, which belongs to the technical fields of prevention and treatment of animal infectious diseases, is named as Mycoplasma bovis T9.171, and is preserved in China Center for Type Culture Collection (CCTCC). The preservation number of the Mbov_0570 gene is CCTCC NO: M 2020083, and the nucleotide sequence of the Mbov_0570 gene isshown as SEQ ID NO: 1. According to the invention, qRT-PCR is used for detecting the capacity of T9.171 strain for stimulating BoMac cells to express IL-8, which shows that the capacity of the mutantstrain for inducing the BoMac cells to express cytokines is reduced, so that the toxicity of the mutant strain is reduced, and the mutant strain is expected to play an important role in the fields ofmycoplasma bovis immune prevention and treatment as a vaccine strain.

The invention discloses a product, method and application for simultaneously detecting mycoplasma gallisepticum and mycoplasma synoviae. According to the product and the method, a common specific protein antigen of mycoplasma gallisepticum and mycoplasma synoviae is preferably an outer membrane protein WP-041352022.1 as a detection object, a PCR primer pair S1 is designed and screened on the genome DNA level of the protein, and the PCR primer pair has the advantages of strong specificity, high sensitivity and no primer dimer generation. The invention provides a product and a method for simultaneously detecting two pathogens by using one pair of PCR primers, mutual interference between multiple PCR primers and the product is avoided, the detection rate is improved, and the method has the advantages of economy, simplicity, convenience and high efficiency.

The invention relates to a mycoplasma pneumoniae recombinant antigen and application thereof. The mycoplasma pneumoniae recombinant antigen comprises a PIA fragment, a first binding peptide, a PIB fragment, a second binding peptide and a P30 fragment. Experiment results show that the recombinant antigen can obtain different types of antigen dominant epitopes after being purified in different ways,and the various types of antigen dominant epitopes are stable in expression, namely the different types of antigen dominant epitopes can be stably expressed as required. The matching and screening works of the antigen can be greatly reduced during detection; the detection rate is high, and the specificity is high during the detection of mycoplasma pneumoniae.

The invention provides a hybridoma cell strain capable of secreting an anti-mycoplasma bovis monoclonal antibody and application thereof.The hybridoma cell strain can produce the anti-mycoplasma bovis monoclonal antibody and is used for detecting mycoplasma bovis.The preservation number of the hybridoma cell strain is CCTCC C2015183, and the preservation date is November 8, 2015.The hybridoma cell strain has the advantages that the hybridoma cell strain can specifically secrete the anti-mycoplasma bovis monoclonal antibody and has better sensitivity and higher specificity.The hybridoma cell strain further has the advantages that the antibody produced by the cell strain can be applied in a variety of ways and has a good application value during actual production.

The invention provides a method for coating mycoplasma pneumoniae membrane protein antigens with magnetic beads, and relates to the technical field of biology. The mycoplasma pneumoniae membrane protein antigens coated by the method are natural mycoplasma pneumoniae protein. The method comprises the following steps: treating the mycoplasma pneumoniae membrane protein antigens by using a reducing agent, and coupling the mycoplasma pneumoniae membrane protein antigens with magnetic beads. According to the method, the technical problem that the efficiency of coating the magnetic beads with the natural mycoplasma pneumoniae membrane protein antigen is low is alleviated.

The invention discloses a mutant strain of a mycoplasma bovis Mbov_0475 gene, which belongs to the technical field of animal infectious disease prevention. The mutant strain is named as Mycoplasma bovis T9.55, and is preserved in China Center for Type Culture Collection, a preservation number is CCTCC NO: M 2020082, and the nucleotide sequence of the Mbov_0475 gene is shown as SEQ ID NO: 1. The strain has the effects of inducing macrophage proliferation and improving macrophage activity, and because bovine macrophages are important immune cells of a host for resisting pathogenic infection, themutant strain is expected to play an important role in the field of mycoplasma bovis immune prevention and treatment.

The invention discloses a traditional Chinese medicine composition for preventing and treating swine enzootic pneumonia. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 6 parts of radix ophiopogonis, 6 parts of thunberg fritillary bulb, 6 parts of astragalus membranaceus, 6 parts of aristolochia debilis, 4 parts of scutellaria, 4 parts of cortex mori radicis, 4 parts of schisandra chinensis, 3 parts of honeysuckle, 3 parts of rhizoma anemarrhenae, 3 parts of bighead atractylodes rhizome, 3 parts of semen lepidii and 2 parts of radix glycyrrhizae. The invention further provides a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition for preventing and treating swine enzootic pneumonia has the benefits and advantages that the swine enzootic pneumonia can be effectively prevented and treated, and the preventing and treating effects are good; (2) the medicine can be fed by adopting water drinking or feed mixing, the operation is simple and the cost is low; (3) as the traditional Chinese medicine composition is Chinese herbal medicine preparation, the problems of drug tolerance and the like are avoided, and the probability of preventing and treating failure is greatly reduced; (4) the traditional Chinese medicine composition is pure traditional Chinese medicine compound, and has the advantages of multiple target, low residue in swine and good biosecurity when being acted on mycoplasma.

The invention discloses a protein encoded by a mycoplasma bovis Mbov-0145 gene. The mycoplasma bovis Mbov-0145 gene has a nucleotide sequence as shown in SEQ ID NO: 1. The invention also discloses anantibody of the gene, and an application of the protein and the antibody in preparation of a mycoplasma bovis detection kit, and belongs to the technical field of prevention and treatment of animal infectious diseases. The protein can generate specific reaction with positive serum of naturally infected and artificially infected cattle of a mycoplasma bovis virulence strain M.bovis HB0801, and canidentify animals infected with wild virus after vaccine immunization. The protein is expected to be used as a molecular target for diagnosis reagent and vaccine of mycoplasma bovis infection and drugresearch and development, and plays an important role in prevention and control of mycoplasma bovis.

The invention relates to a traditional Chinese medicine for treating non-gonococcal urethritis, and is used for effectively solving the problem of medicines for treating the non-gonococcal urethritis. A method is as follows: the traditional Chinese medicine is prepared from the following Chinese medicinal herbs of wine fried felwort, lichen of parmelia saxitilis, Chinese mahonia, rheum officinale, stir-baked squama manitis, fried semen plantaginis, rhizoma alismatis, radix bupleuri, wine fried angelica sinensis, fried cape jasmine, wine fried scutellaria baicalensis, dianthus superbus, polygonum aviculare, broom cypress fruit, golden cypress, ural licorice root tip, rhizoma anemarrhenae, pseudo-ginseng, cordyceps sinensis, poria cocos, trollflower, fried bighead atractylodes rhizome, ligusticum wallichii, dipsacus root, medicinal cyathula root, radix pseudostellariae, the root bark of the peony tree, amber, rhizoma atractylodis, eucommia bark, morinda meat, radix stemonae, lychee seed and common peony root. The traditional Chinese medicine is scientific in compatibility, good in treatment effect, safe and reliable, integrates a nourishing function into a removing function, integrates a releasing function in the nourishing function so as not to hurt healthy qi and attach pathogenic qi, has the functions of clearing away heat and toxic materials, sterilizing and diminishing inflammation, inducing diuresis for treating strangurtia, promoting blood circulation to remove blood stasis, resolving hard lump, tonifying liver and kidney, strengthening the spleen and stomach and improving the immunity, and is mainly used for treating non-gonococcal mycoplasma and chlamydia microbial infection, male mixed prostatitis and orchitis, female mixed cervicitis and pelvic inflammation, immunodeficiency, and the like.

Provided is a method for culturing cells, which prevents the possibility of mycoplasma contamination by culturing the cells using biomass extract obtained by culturing a strain with amphidinol productivity, preferably a strain such as Amphidinium klebsii, Amphididinium carterae, and the like belonging to Dinoflagellates, or using a fraction obtained from the extract; and a method for removing mycoplasma contamination of the cells by culturing the cells contaminated by mycoplasma using the amphidinol derivatives. In addition, provided is a method for removing mycoplasma contamination of the cells, including a combination of single cell-isolating enzyme solution treatment and cell aggregate removal.

The invention provides a kit for rapid detection and genotyping of mycoplasma pneumoniae, belonging to the technical field of microbiological detection. The kit contains 2 specific primer pairs and 2 probes matched with the primer pairs, and the nucleotide sequences are shown by SEQ ID No.1-6 respectively; rapid detection and genotyping of mycoplasma pneumoniae are realized by a duplex fluorescent quantitative PCR (polymerase chain reaction) method, the detection sensitivity is 3CFU, the coincident rate between the genotyping accuracy and that of common genotyping methods reaches 100%, the genotyping sensitivity is much higher than that of common culture-dependent genotyping technologies, the genotyping time is greatly shortened, and the genotyping can be performed at the time of detection on mycoplasma pneumonia in a sample; and moreover, the shortcomings of low sensitivity, time and labor consuming and relatively high cost of the existing mycoplasma pneumoniae genotyping method are overcome, and the kit provided by the invention has good practical sample detection and genotyping ability and is suitable for popularization and application.

The invention provides a mycoplasma pneumoniae fusion antigen as well as a preparation method and application thereof, and relates to the technical field of biology. The mycoplasma pneumoniae fusion antigen is a fusion protein containing a P1M antigen fragment and a P30A antigen fragment. The mycoplasma pneumoniae fusion antigen provided by the invention is verified by an immunoserological detection technology, and compared with an existing mycoplasma pneumoniae antigen, the mycoplasma pneumoniae fusion antigen is stronger in specificity, higher in sensitivity, easy to culture and purify, morebeneficial to industrial production and lower in cost. The mycoplasma pneumoniae fusion antigen is suitable for preparation of MP antibody detection products, can be processed into products in any form in the field of in-vitro immunodiagnosis, and has a wide market prospect.

The invention provides a Mycoplasma hyorhinis strain LYH, and a vaccine composition prepared from the Mycoplasma hyorhinis strain LYH, in particular to a vaccine composition comprising the Mycoplasma hyorhinis and Mycoplasma hyopneumoniae. The vaccine composition can be effective in prevention and treatment of swine enzootic pneumonia caused by Mycoplasma hyorhinis, Mycoplasma hyopneumoniae single infection or mixed infection. Especially in the circumstance of mixed infection, immune effect of the vaccine composition significantly exceeds that of each single vaccine.

The invention discloses a mycoplasma hyopneumoniae genetic engineering subunit vaccine as well as a preparation method and an application thereof. The vaccine comprises a protein composition and a pharmaceutically acceptable carrier, wherein the protein composition comprises two fusion proteins with sequences shown in SEQ ID NO: 2 and SEQ ID NO: 6 respectively. The vaccine provided by the invention has no toxicity; the vaccine is high in safety, good in immunogenicity and capable of generating strong humoral immunity in pig bodies, immunized animals can resist poison attacking of strong poisonand are comprehensively protected, and the vaccine can be prepared through large-scale serum-free suspension culture of a bioreactor and has the advantages of being easy to control in quality, stablebetween batches, low in production cost and the like.

The invention discloses a mycoplasma bovis VspX gene mutant strain. The mycoplasma bovis VspX gene mutant strain is named as Mycoplasma bovis T8.101, is preserved in the China Center for Type CultureCollection (CCTCC), and has a preservation number of CCTCC NO: M 20191038, and the VspX gene has a nucleotide sequence shown as SEQ ID NO:1. The invention also discloses a construction method of the mutant strain and an application of the mutant strain in the fields of mycoplasma bovis pathogenic mechanism and immune prevention and control. The mutant strain does not express protein coded by the mutant gene, and the growth curve, colony morphology and size of the mutant strain are not obviously different from those of a wild strain. However, the binding capacity of the mutant strain to bovinepulmonary epithelial cell lines and fibronectin is remarkably reduced, so that the mutant strain has the potential of being applied to the fields of bovine mycoplasma pathogenic mechanism research andprevention and treatment drug research and development as a virulence attenuating strain.

The invention belongs to the field of animal bacteria engineering technology, and specially relates to a construction of a resistance marker-free recombinant attenuated Salmonella choleraesuis for expressing a main immunogenic membrane protein of mycoplasma hyopneumoniae, a vaccine preparation and an application thereof. The resistance marker-free attenuated Salmonella choleraesuis C500(pYA-65) for expressing mycoplasma hyopneumoniae p65 protein is obtained, and the collection number is CCTCC NO: M2011107. An asd gene necessary for the growth of Salmonella choleraesuis is deleted in the attenuated strain, and the attenuated strain contains a plasmid capable of expressing the asd gene and the p65 gene of mycoplasma hyopneumoniae. The invention further discloses a Salmonella choleraesuis and mycoplasma hyopneumoniae vaccine prepared from the attenuated strain, a preparation method and an application thereof. The bivalent vaccine of the invention can stimulate a swine to generate a protective immune response resisting the Salmonella choleraesuis and mycoplasma hyopneumoniae, and effectively prevent infection of the Salmonella choleraesuis and mycoplasma hyopneumoniae.