Preparing method and application of mycoplasma bovis inactivated vaccine

Abstract

The invention discloses a preparing method of a mycoplasma bovis inactivated vaccine. By studying the formaldehyde inactivation condition for preparing the mycoplasma bovis inactivated vaccine, the immunizing dose of the mycoplasma bovis inactivated vaccine, the immunization way of the mycoplasma bovis inactivated vaccine, the adjuvant adopted by the mycoplasma bovis inactivated vaccine, and clinical application of the mycoplasma bovis inactivated vaccine, the optimal mycoplasma bovis formaldehyde inactivation condition, the optimal immunizing dose of the mycoplasma bovis inactivated vaccine, the optimal immunization way of the mycoplasma bovis inactivated vaccine, and the optimal immunization adjuvant are found out, then clinical animal experiments are conducted, and the results show that the mycoplasma bovis inactivated vaccine has a good effect on prevention of mycoplasma bovis diseases in clinical application, can be used as a clinically popularized vaccine, and lays a scientific foundation for prevention of mycoplasma bovis diseases.

Description

technical field [0001] The invention relates to a preparation method of an inactivated vaccine of mycoplasma bovis. Background technique [0002] Mycoplasma bovis (Mycoplasma bovis) is one of the important pathogenic pathogens that cause multi-system inflammation such as respiratory tract inflammation, mastitis and arthritis in cattle. In 1961, Hale et al. first isolated the pathogen from the milk of cows suffering from mastitis in the United States; since then, Mycoplasma bovis has been isolated in Ireland, South America and Europe. In 2008, an outbreak of "Infectious Mycoplasma bovis pneumonia" characterized by necrotizing pneumonia broke out in Hubei Province of my country. Later, the disease was found in calves introduced in Hubei, Guizhou, Ningxia, Inner Mongolia, Guangxi, Chongqing and other regions. The disease rate is 50%~100%, and the case fatality rate is as high as 10%~50%, which has caused huge economic losses to my country's cattle industry. The immune mechanis...

AI Technical Summary

Problems solved by technology

In 1987, Barite et al. used hamsters to test the Mycoplasma bovis vaccine and the inactivated formalin vaccine. The two vaccines can prevent Mycoplasma bovis pneumonia, but the interaction between the two cannot be distinguished

Although there are two commercial vaccines in the United States, in 2011 someone tested the protective properties of these two vaccines. The 27d calf immunity test showed that one of the two vaccines can significantly improve the antibody level, but the protection of cattle The rate is about 44%; the immune effect of another vaccine is not significant

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