33 results about "Chromaffin cell" patented technology

The invention relates to bifidobacterium breve CCFM1025, a fermented food and application thereof. The bifidobacterium breve CCFM1025 can be used for improving the depression-like behavior of a depression mouse, improving 5-hydroxytryptamine in the brain of the depression mouse, improving the level of 5-hydroxytryptamine and a brain-derived neurotrophic factor, reducing the level of corticosteronein serum of the depression mouse, improving the level of 5-hydroxytryptamine in the serum of the depression mouse, improving intestinal flora disturbance of the depression mouse, reducing the abundance of intestinal veillonella, improving the abundance of bifidobacterium and mycoplasmataceae, improving alpha-diversity of intestinal flora and reducing occurrence of inflammatory bowel disease and obesity. By adopting the bifidobacterium breve CCFM1025, the mRNA level of tryptophan hydroxylase in a simulated entero-chromaffin cell can be improved, the secretion volume of 5-hydroxytryptophane ofthe cell can be improved, and a precursor substance is specifically provided to synthesis of 5-hydroxytryptamine in the brain. The bifidobacterium breve CCFM1025 has a wide application prospect.

The invention discloses application of artemisinin and derivatives thereof in preparing medicaments for preventing and treating neurological diseases. In the application, the nerve cell line of rat adrenal pheochromocytoma PC12, retinal nerve cell line RGC-5 and primary cortical neuron are on behalf of experiment objects, sodium nitroprusside and hydrogen peroxide are used to simulate and induce oxidative stress, cell apoptosis and other cell injuries of nerve cells. Researches discover that the artemisinin has a protective effect on sodium nitroprusside and hydrogen peroxide induced cell injury, the protective effect mechanism of the artemisinin and derivatives thereof is primarily discussed, and results indicate that the artemisinin and derivatives thereof can be used as nerve protection medicaments for preventing and treating various neurological diseases, such as various neurodegenerative diseases, acute and chronic neurodegenerative diseases, neurological eye disease and the those neurological diseases mainly related to oxidative stress injuries and cell apoptosis. The application brings a new direction to treatment and prevention of neurological diseases.

The present invention provides methods, compounds and kits for increasing the viability of cells. The methods involve treating cells that make up a tissue graft with erythropoietin before, during or after delivery or administration. The method can employ cells of different types, including cells of neural or paraneural origin, such as adrenal chromaffin cells. Also useful are cell lines grown in vitro. Cells not of neural or paraneural origin, such as fibroblasts, may also be used following genetic alteration to express a desired neural product such as a neurotransmitter or a neuronal growth factor. The method is used to treat neurological diseases such as Parkinson's disease, Alzheimer's disease, Huntington's disease, epilepsy, and traumatic brain or spinal cord injury.

The invention provides a method for extracting artemisinin. The artemisinin is prepared through the method of supercritical fluid extraction and the method of microwave-assisted extraction, and the content of the artemisinin is greatly improved. The invention further provides the application of the artemisinin in preparing medicines for inhibiting proliferation of rat adrenal gland pheochromocytoma cell PC-12 and the application of the artemisnin in preparing medicines for increasing bone mineral density.

The invention provides a traditional Chinese medicinal material bio-based carbon nanodot. The bio-based carbon nanodot is a ginseng total saponin carbon nanodot. The carbon dot surface of the total ginsenoside is rich in alcohol/phenolic hydroxyl, amino, carboxyl and other structures, the surface groups are rich, subsequent modification and compounding are facilitated, and the total ginsenoside has good time, pH value and salt stability; various cell fluorescence imaging experiments prove that the total ginsenoside carbon dots can be used as a biological fluorescence probe; meanwhile, the obtained total ginsenoside carbon dots have a specific inhibition effect on rat adrenal pheochromocytoma cells (PC12 cells), and have no obvious proliferation or inhibition effect on other cells (HeLa cells, BRL3A cells and LO2 cells); meanwhile, the total ginsenoside carbon dots can be used as fluorescent probes and can be taken by various cells (BRL3A cells, LO2 cells and QGY7703 cells).

The invention discloses a PCR primer used for building a library for chromaffin cell pathogenic gene next generation sequencing and a library building method, wherein the PCR primer is selected from nucleotides shown in SEQ ID NO:1-SEQ ID NO:70; and chromaffin cell pathogenic genes are SDHB, SDHC, SDHD, MAX, VHL, TMEM127 and RET. The library building method comprises the following steps: utilizing extracted human genome DNA as a template, adopting the primer as a specific amplification part of the primer to carry out first-round PCR amplification; diluting a product of the-first PCR amplification as a template of second-round PCR amplification; carrying out the second-round PCR amplification, namely carrying out another round of amplification by utilizing a universal primer of the first-round PCR amplification, and adding index required by a sequencing machine and a base sequence used for identifying the sequencing machine in a specific primer identification zone; and purifying a second-round sequencing product with nucleic acid, so that the second-round sequencing product can be subjected to sequencing on the sequencing machine. The library building method disclosed by the invention is designed and developed self and is extremely low in cost compared with a purchased foreign kit.

The invention provides a preparation method of total steroidal saponin extracts of dwarf lilyturf roots, which comprises the following steps: crushing traditional Chinese medicine dwarf lilyturf roots, leaching with methanol, concentrating through vacuum filtration, separating through an ODS column, and finally separating through a silica gel chromatography open column. Based on an anti-senile dementia in-vitro screening model, experiment on rat adrenal pheochromocytoma cells proves that the total steroidal saponin extracts of dwarf lilyturf roots have activity remarkably similar to that of a nerve growth factor (NGF) and can promote nerve cells to grow out and protrude. Thus, the invention is applicable to the preparation of medicaments for preventing and treating senile dementia, especially medicaments for treating Alzheimer diseases and other neurodegenerative diseases. The invention expands the pharmaceutical application of the total steroidal saponin extracts of dwarf lilyturf roots; the preparation method is convenient; the extracted product has high purity; and dwarf lilyturf roots have extensive sources and can be acquired conveniently.

The invention provides an extracting method of a plant composite containing gastrodia elata. The plant composite is prepared from the raw material herbs of 100 g of gastrodia elata, 60 g of pteris biaurita, 50 g of ageratum conyzoides and 150 g of lysimachia fortunei maxim. The plant composite is prepared by the adoption of the microwave extracting method, in this way, the content is greatly increased, and the usage amount is reduced. The invention further provides an application of the plant composite containing the gastrodia elata in preparation of medicine for restraining rat adrenal medulla pheochromocytoma PC12 cell proliferation.

The invention belongs to the technical field of medicines and biology, and particularly relates to an extraction method and application of gastrodia elata oligosaccharide GEP-2. The invention provides an extraction method of gastrodia elata oligosaccharide GEP-2. The gastrodia elata oligosaccharide GEP-2 provided by the invention has a similar net-shaped structure for preparing a dissolution assisting platform, and the dissolution state of an extract can be remarkably improved. Meanwhile, the gastrodia elata oligosaccharide GEP-2 also has excellent activity similar to nerve growth factors (NGF), and can induce nerve cell differentiation and promote pheochromocytoma (PC12) differentiation. Tests prove that the gastrodia elata oligosaccharide GEP-2 provided by the invention can effectively improve the differentiation rate of nerve cells.

The invention provides a preparation method of an active component extracted from traditional Chinese medicine, namely, radix ophiopogonis, an active saponin compound contained in the component, and a separation and preparation method of the compound. The identification result shows that the active component of the radix ophiopogonis contains four active compounds. According to the experiment for an anti-senile dementia in vitro screening model, namely, rat pheochromocytoma cells, the active component of radix ophiopogonis and the four active components show remarkable nerve growth factor simulating activity, namely, NGFmimics, which can promote the outgrowth and protuberance of nerve cells. The preparation method has the advantages that the medical application of the active component of radix ophiopogonis and the active compound contained in the component in prevention of senile dementia is created; and a new treatment medicine is provided for preventing and treating senile dementia and other neurodegenerative diseases.

The invention relates to a Cannabis-based pharmaceutical composition for the treatment of hypertensive disorders by submucosal delivery comprising a pharmaceutically acceptable base and an effective amount of at least one cannabinoid or endocannabinoid containing extract of a cloned hybrid of the plant Cannabis sativa, subspecies sativa and Cannabis sativa, subspecies indica of the CTSX-ISS lineage; and methods of treatment of primary and secondary hypertension, the secondary hypertension resulting from pheochromocytoma, primary hyperaldosteronism, adrenal hyperplasia, pulmonary hypertension, portal hypertension, folate deficiency hypertension, arterial hypertension or familial hypertension by administration between one and eight times per day.

The present invention relates to a new application of adrenal medulla chromaffin cells or opioid peptidergic cells, especially for treating and/or alleviating withdrawal syndrome caused by drug addiction or drug abuse.

The invention discloses a preparation method of an ordered porous nerve conduit material. Aiming at the difficulties of low mechanical strength, non-uniformity, difficulty in organic solvent removal and the like of materials in preparation of different nerve conduit materials, the invention prepares the ordered porous catechol-modified chitosan nerve conduit three-dimensional scaffold material bya directional freeze-drying means. By doping graphene oxide, the nerve conduit material has certain conductivity, and meanwhile, the mechanical property of the material is enhanced, so that the high-strength ordered porous nerve conduit three-dimensional scaffold material is formed. Meanwhile, highly-differentiated pheochromocytoma cells (PC12) are used for monitoring the cell migration and proliferation process, and a way is provided for material selection of nerve conduit transplantation and nerve regeneration medical application.