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Bifidobacterium breve ccfm1025, its fermented food and its application

A technology of CCFM1025 and Bifidobacterium breve, which is applied in the field of microorganisms, can solve the problems of reduced synthesis and secretion of 5-HT, reduced compliance of patients taking medication, and no preventive effect, so as to relieve HPA hyperfunction and improve intestinal peristalsis function , reducing the incidence of inflammatory bowel disease and obesity

Active Publication Date: 2020-08-04
无锡食生臻选生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are two problems in the current clinical medication: first, there is a 2-4 week delay in the onset of the drug. The reason is that after taking SSRI to increase the concentration of 5-HT in the synaptic cleft, 5-HT will react on the presynaptic 5-HT1A receptors, which produce negative feedback inhibition, resulting in decreased synthesis and secretion of presynaptic 5-HT
And these 2-4 weeks will lead to a decrease in patients' medication compliance in clinical practice and greatly increase the risk of suicide
Second, most antidepressants do not have a preventive effect, and long-term medication can cause side effects such as non-specific abdominal pain, constipation, diarrhea, indigestion, and flatulence

Method used

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  • Bifidobacterium breve ccfm1025, its fermented food and its application
  • Bifidobacterium breve ccfm1025, its fermented food and its application
  • Bifidobacterium breve ccfm1025, its fermented food and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: Bifidobacterium breve CCFM1025 can significantly improve the behavioral indicators of depressed mice

[0070] Thirty-two 6-week-old male C57BL / 6J mice were randomly divided into four groups according to body weight after one week of acclimatization: control group, model group, fluoxetine intervention group, and CCFM1025 intervention group, with 8 mice in each group. The animal groups and treatment methods are shown in Table 1.

[0071] Table 1 Grouping and processing methods of animal experiments

[0072]

[0073] Chronic unpredictable stress depression mouse model: 1-2 kinds of stimuli are randomly used every day, and the time of stimulation is randomly determined every day to avoid circadian rhythm. No more than three times of each method for a period of five weeks. Stimulus factors include: (1) fasting for 24 hours; (2) water deprivation + empty bottle stimulation for 24 hours; (3) tail clamping for 3 minutes; (4) wet litter for 24 hours; (5) braking ...

Embodiment 2

[0082] Example 2: CCFM1025 can significantly increase the level of neurotransmitters in the brain of depressed mice

[0083] The mice in Example 2 were euthanized at the end of the sixth week, and the brain tissues of the mice were taken, and the hippocampus and prefrontal cortex were separated on ice. Take a certain quality of fresh hippocampus and prefrontal cortex tissue (weight not less than 50mg), add 9 times the volume of sterile PBS buffer (equivalent to 1g tissue plus 9ml homogenate), and homogenize with a tissue homogenizer , The tissue fluid was centrifuged at 3000g for 20min, and the supernatant was taken, and the content of 5-HT and BDNF was detected by ELISA kit. Add an equal volume of 5% perchloric acid to precipitate protein in the supernatant of hippocampal tissue fluid, centrifuge at 10,000 g for 10 min, absorb the supernatant and filter it through a 0.22 μm water filter membrane, and perform 5- Determination of HTP content. Shimadzu IntertsilODS-3 (5 μm, 4....

Embodiment 3

[0084] Example 3: Bifidobacterium breve CCFM1025 can reduce corticosterone levels in mouse serum

[0085] The mice in Example 2 were euthanized at the end of the sixth week, the blood of the mice was collected, and the serum was obtained by centrifugation at 1000 g for 15 min. ELISA kit was used to detect the content of corticosterone in serum. Experimental results show that( image 3 ), the hypothalamic-pituitary-adrenal axis (HPA) hyperfunction in depressed mice due to continuous chronic stress, the corticosterone level in the serum was significantly increased, taking CCFM1025 can significantly reduce the corticosterone level in the serum of depressed mice, Relieves HPA hyperactivity, showing good antidepressant efficacy.

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Abstract

The present invention relates to bifidobacterium breve CCFM1025, its fermented food and application thereof. Bifidobacterium breve CCFM1025 of the present invention can improve the depression-like behavior of depressed mice, increase the levels of 5-hydroxytryptophan, 5-hydroxytryptophan and brain-derived neurotrophic factor in the brain of depressed mice, and reduce the levels of 5-hydroxytryptophan in the serum of depressed mice. Corticosterone levels, increased serum 5-hydroxytryptamine levels in depressed mice, improved intestinal flora disturbance in depressed mice, decreased intestinal Veillonellaceae abundance, increased the abundance of Bifidobacteria and Mycoplasmaceae , increase the α-diversity of intestinal flora, reduce the occurrence of inflammatory bowel disease and obesity; increase the mRNA level of tryptophan hydroxylase 1 in simulated enterochromaffin cells, and increase the level of 5-hydroxytryptophan in this cell Secretion, specifically providing precursors for the synthesis of 5-HT in the brain. It has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Bifidobacterium breve CCFM1025, fermented food and applications thereof. Background technique [0002] Depression, also known as depressive disorder, includes major depressive disorder, bipolar disorder, seasonal affective disorder, postpartum depression, etc. As a heterogeneous disease, the pathogenesis of depression has not been clearly elucidated so far. The inconsistency in response to treatment among individuals with depression makes it a growing global burden of disease. Currently, there are more than 350 million depressed patients in the world, and it is estimated that by 2030, depression will become the number one disease in the world. [0003] There are currently two major explanations for the mechanism of depression, including dysregulation of monoamine neurotransmitters in the brain and dysfunction of the hypothalamic-pituitary-adrenal axis (HPA). ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/12A23C19/032A23C11/10A23C20/02A23L19/00A61K35/745A61P25/24A61P1/00A61P29/00A61P3/04C12R1/01A23L11/00A23L11/45
CPCA61P1/00A61P3/04A61P25/24A61P29/00A23C9/1234A23C11/106A23C19/0323A23C20/025A23L19/00C12N1/20A61K2035/115A23V2002/00C12R2001/01C12N1/205A23V2200/30A23V2400/519
Inventor 陈卫王刚田培郡赵建新张灏
Owner 无锡食生臻选生物科技有限公司
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