14 results about "Hydroxytryptophol" patented technology

The application relates to a reagent combination for detecting depression, a manufacturing method, a detection system and application thereof, and belongs to the technical field of instrument detection. The reagent combination for detecting depression comprises a calibrator, a quality control sample and an extraction solution of a to-be-detected compound serving as a biomarker; wherein the to-be-detected compound is at least three kinds selected from 5-hydroxyanthranilic acid, methionine, gamma-aminobutyric acid, 3,4-dihydroxyphenylacetic acid, 2-picolinic acid, quinolinic acid, kynurenine, 5-hydroxytryptamine, 3-hydroxykynurenine, 3-hydroxyanthranilic acid, dopamine and norepinephrine. The reagent combination for detecting depression and the detection system can significantly improve the accuracy of diagnosis, are convenient to detect, have good repeatability of detection data, are high in specificity, and are accurate in clinical diagnosis results.

The invention provides a genetic engineering strain, a construction method of the genetic engineering strain and application of the genetic engineering strain in biosynthesis of 5-hydroxytryptophan. According to the engineering bacterium, four key modules required by 5-HTP synthesis are sequentially introduced into a host genome in a genome integration mode through a CRISPR technology, and the four key modules comprise an aroGfbr-ppsA-tktA module for enhancing chorismate precursor supply, a trpEfbr-trpG module for catalyzing o-aminobenzoic acid generation, a salABCD gene cluster responsible for hydroxylating o-aminobenzoic acid into 5-hydroxy o-aminobenzoic acid, and a salABCD gene cluster responsible for hydroxylating 5-hydroxy o-aminobenzoic acid into 5-hydroxy o-aminobenzoic acid. And a trpDCBA module for converting the intermediate into the final product 5-HTP. The modules are respectively integrated into a plurality of non-essential gene regions, so that stable genetic expression is realized, and a complete metabolic pathway can be operated without plasmids or inducers. The strain can continuously accumulate 5-hydroxytryptophan (5-HTP) under a glucose fermentation condition, and has the advantages of simplicity and convenience in construction, high hereditary stability, low production cost, suitability for industrial-scale fermentation and the like.

The application discloses an engineering strain for producing 5-hydroxytryptophan and a construction method and application thereof, and the construction steps of the engineering strain are as follows: a recombinant plasmid PACYC-Duet1-FolE-PTPS-SPR-PCD-DHPR-TPH1-GDH is constructed; the plasmid is introduced into escherichia coli or vibrio natriegens to obtain an engineering strain for producing 5-hydroxytryptophan; the engineering strain for producing 5-hydroxytryptophan is fermented to obtain 5-hydroxytryptophan, and the production efficiency is high, and the production cost is reduced; and the conversion efficiency of L-tryptophan as a substrate is above 88.6%.

The present invention discloses a SAMe, 5-HTP, and St. John's wort compound nutritional supplement for emotional support and joint care, comprising 15-25 parts of S-adenosylmethionine, 5-10 parts of 5-hydroxytryptophan, 3-8 parts of St. John's wort extract, 8-15 parts of sodium chondroitin sulfate, 2-5 parts of sodium hyaluronate, 0.5-1.2 parts of vitamin B6, 1-3 parts of vitamin E, 4-9 parts of phosphatidylserine, and pharmaceutical excipients. The weight ratio of S-adenosylmethionine to 5-hydroxytryptophan is 3:1-5:2, the weight ratio of St. John's wort extract to 5-hydroxytryptophan is 0.6:1-0.8:1, and the weight ratio of the joint-care agents to the total core active ingredients is 10:3-20:3. The hypericin content in the St. John's wort extract is not less than 3%. By optimizing the component ratios and utilizing synergistic functional excipients, the present invention achieves dual synergy in emotional support and joint care, with high product stability and excellent bioavailability.

The present invention discloses a sleep-aid composition for synergistically regulating the sleep rhythm with 5-HTP and L-theanine, relating to the technical field of biomedicine and health foods. Through the synergistic action of 5-hydroxytryptophan, L-theanine, and gamma-aminobutyric acid, the present invention effectively regulates the sleep rhythm and helps users improve sleep quality. As a precursor of serotonin, 5-HTP helps increase the serotonin level and is then converted into melatonin to regulate the sleep cycle. L-theanine promotes the release of alpha brain waves to help relax the body and mind and reduce anxiety. As an inhibitory neurotransmitter, GABA can effectively relieve tension and promote deep sleep. The three components act synergistically to provide users with more comprehensive and more effective sleep support.

The application discloses a composite sleep-aiding patch, a preparation method and application, and belongs to the technical field of sleep-aiding patches.The composite sleep-aiding patch comprises the following raw materials in percentage by weight: 0.5-1% of melatonin, 1-2% of L-theanine, 0.5-1% of 5-hydroxytryptophan, 2-3% of gamma-aminobutyric acid, 0.5-1% of linalyl acetate, 1-2% of valeric acid, 1-2% of vitamin E and 1-2% of rosmarinic acid, and the rest is auxiliary materials.The composite sleep-aiding patch can help sleep, improve the depth and quality of sleep, and has the synergistic effect of different components.

The present invention provides a composition, a method of making the composition, and a method of using such composition for facilitating prevention of snoring and sleep apnea. The disclosed composition includes at least one active ingredient selected from Magnesium BHB, Calcium BHB, Sodium BHB, or 5-Hydroxytryptophan. According to an exemplary embodiment of the present invention, Silentium, which comprises one of the disclosed compositions, stops snoring and cures sleep apnea. The composition may be prepared by first adding and blending Magnesium BHB, then Calcium BHB, then Sodium BHB, and lastly, 5-Hydroxytryptophan. The composition may be taken orally in the form of a capsule or in the form of a solution.

A method for enhancing 5-hydroxytryptophan secretion by enterochromaffin cells is disclosed, and the method comprises: administering a composition to a subject in need thereof, the composition comprising: a Bifidobacterium animalis subsp. lactis BB-115 strain. Herein, another method for enhancing 5-hydroxytryptophan secretion by enterochromaffin cells through administering a composition to a subject in need thereof, the composition comprising: a Bifidobacterium animalis subsp. lactis BB-115 strain, a Lactobacillus rhamnosus MP108 strain, and a Bifidobacterium longum subsp. infantis BLI-02 strain.

The present invention relates to novel processes and compounds for synthesizing amanitin derivatives. The invention relates in particular to processes for synthesizing (S)-hydroxytryptophan derivatives that can be used as building blocks in the synthesis of amanitin derivatives or amatoxin-drug conjugates. The invention also relates to intermediate compounds of said synthetic routes for use in the synthesis of amanitin derivatives and amatoxin-drug conjugates, as well as to the use of suitable catalysts for mediating said synthetic routes.

The invention discloses a preparation method of astragalus mongholicus fermented polysaccharide and application of the astragalus mongholicus fermented polysaccharide in medicines and foods for intervening premenstrual syndrome, relates to a preparation method and application of an astragalus mongholicus fermented product, and belongs to the technical field of crossing of traditional Chinese medicines and microorganisms. The preparation method comprises the following steps: inoculating the lactobacillus casei paracasei into an astragalus membranaceus culture medium according to the inoculum size of 5% under the culture condition of 37 DEG C; the fermented astragalus polysaccharide is used as an active ingredient in the premenstrual syndrome intervention medicines and foods for intervening the premenstrual syndrome. The astragalus membranaceus fermented polysaccharide is used for gavage of mice, has better biological activity of relieving premenstrual syndrome related emotional disorder, and is mainly used for relieving neuronal injury in brain tissues by increasing the weight of the premenstrual syndrome mice and the content of gamma-aminobutyric acid, tetrahydroprogesterone, 5-hydroxytryptophan and brain-derived neurotrophic factors, so that the premenstrual syndrome related emotional disorder can be relieved. The aim of treating the premenstrual syndrome is fulfilled.

The invention discloses streptomyces albus genetically engineered bacteria with high yield of 5-hydroxytryptamine as well as a construction method and application of the streptomyces albus genetically engineered bacteria, and belongs to the technical field of microbial genetic engineering and fermentation engineering. The genetically engineered bacterium is constructed by taking streptomyces albus of which tryptophan bioxidase is knocked out as a starting bacterium, introducing a tryptophan hydroxylase coding gene and a 5-hydroxytryptophan decarboxylase coding gene and knocking out at least one of acyltransferase genes ata1, ata2, ata3, ata4 and ata5 in a genome of the streptomyces albus while knocking out the tryptophan hydroxylase coding gene and the 5-hydroxytryptophan decarboxylase coding gene. According to the invention, five key acyltransferase genes ata1-ata5 participating in acetylation of 5-HT in streptomyces albus are knocked out, so that the yield of 5-HT reaches 752.72 + / -94.28 mg / L under laboratory conditions, and an efficient biological manufacturing scheme is provided for industrial production of 5-HT.

Composition including: a) Melatonin up to 5 mg; b) Magnesium up to 500 mg c) Withania somnifera up to 2 g; d) Gamma-aminobutyric acid up to 500 mg; e) L-tryptophan up to 1 g; f) L-Theanine up to 50 mg; g) 5-Hydroxytryptophan up to 100 mg; h) Matricaria chamomilla extract up to 500 mg; i) Valeriana officinalis extract up to 800 mg; and j) Vitamin B6 up to 5 mg.

A kind of algorithm model for depression detection, detection system, program and storage medium.The training method of the artificial intelligence model includes: constructing artificial intelligence model, its input includes the concentration of 3-4 kinds of to-be-detected compounds selected from 5-hydroxyanthranilic acid, methionine, gamma-aminobutyric acid, 3,4-dihydroxyphenylacetic acid, 2-picolinic acid, quinolinic acid, kynurenine, 5-hydroxytryptamine, 3-hydroxykynurenine, 3-hydroxyanthranilic acid, dopamine, norepinephrine, homocysteine, and the output is the risk index of suffering from severe depression;Large sample data is used to train the artificial intelligence model.The AUC of the detection model of the application is greater than or equal to 0.95, even 0.99;Accuracy rate reaches more than 90%;High sensitivity;Algorithm is simple, iterative convergence is fast, is convenient for transplantation, can be embedded into detection system, also can be used as a single data processing unit, works using encryption key or external U disk mode.