Method for extracting artemisinin and application of artemisinin

An extraction method and technology of artemisinin, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc., can solve the problems of affecting application, many impurities, rough process, etc.

Inactive Publication Date: 2013-12-25
NANJING ZHENGLIANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no report on the use of supercritical and microwave technology for the extraction and preparation of artemisinin. However, the above-mentioned method has rough and backward technology and many impurities, which leads to excessive dosage for patients and is inconvenient to take, which seriously affects the production of artemisinin. products in clinical application

Method used

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  • Method for extracting artemisinin and application of artemisinin
  • Method for extracting artemisinin and application of artemisinin

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Take 1000g of Artemisia annua, add to CO 2 In a supercritical extractor, ethanol is used as an entrainer, and the entrainer accounts for 4% by volume of the total extraction solvent. The extraction pressure is 15MPa, the temperature is 30°C, and the CO 2 The flow rate is 1m1 / g crude drug·min, the extraction time is 150min, and the supercritical extract is obtained, then 70% ethanol is added, and put into the microwave extraction device for microwave extraction, the extraction power is 400W, and the extraction is performed twice, each time for 4 minutes, and each time the added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, added to a D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume eluate, recovered ethanol under reduced pressure, concentrated and dried to obtain green Artemisinin extract, the content determined by high performance liquid chromatography is 96.8%.

Embodiment 2

[0020] Take 1000g of Artemisia annua, add to CO 2 In a supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 6%, the extraction pressure is 30MPa, the temperature is 50°C, and the CO 2 The flow rate is 3m1 / g crude drug·min, the extraction time is 180min, and the supercritical extract is obtained, then 70% ethanol is added, and put into a microwave extraction device for microwave extraction, the extraction power is 600W, and the extraction is performed twice, each time for 8 minutes, and each time the added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, added to a D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume eluate, recovered ethanol under reduced pressure, concentrated and dried to obtain green Artemisinin extract, the content determined by high performance liquid chromatography is 97.8%. Example 3

Embodiment 3

[0021] Take 1000g of Artemisia annua, add to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the entrainer accounts for 5% by volume of the total extraction solvent, the extraction pressure is 20MPa, the temperature is 40°C, and the CO 2 The flow rate is 2m1 / g crude drug·min, the extraction time is 160min, and the supercritical extract is obtained, then 70% ethanol is added, and put into the microwave extraction device for microwave extraction, the extraction power is 500W, and the extraction is performed twice, each time for 6 minutes, and each time the added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, added to a D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume eluate, recovered ethanol under reduced pressure, concentrated and dried to obtain green Artemisinin extract, the content determined by high performance liquid chromatography is 97.9%.

[0022] Artemisia annu...

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Abstract

The invention provides a method for extracting artemisinin. The artemisinin is prepared through the method of supercritical fluid extraction and the method of microwave-assisted extraction, and the content of the artemisinin is greatly improved. The invention further provides the application of the artemisinin in preparing medicines for inhibiting proliferation of rat adrenal gland pheochromocytoma cell PC-12 and the application of the artemisnin in preparing medicines for increasing bone mineral density.

Description

technical field [0001] The invention relates to the technical field of extraction of traditional Chinese medicines, in particular to an extraction method and application of artemisinin. Background technique [0002] Artemisinin, the molecular formula of artemisinin is C 15 h 22 o 5 , Molecular weight 282.33, the current method of extracting artemisinin from Artemisia annua is based on the extraction principle, mainly ether extraction method and solvent gasoline extraction method. Volatile oil is mainly extracted by steam distillation and separated by vacuum distillation. The process is: feeding-water-adding-distillation-cooling-oil-water separation-essential oil; non-volatile components are mainly extracted by organic solvent, column chromatography and recrystallization separation, the basic process It is: drying-crushing-soaking, extraction (repeatedly)-concentrated extract-crude product-refining. [0003] In the prior art, there is no report on the use of supercritical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/20A61K31/366A61P19/10
CPCY02P20/54
Inventor 不公告发明人
Owner NANJING ZHENGLIANG MEDICAL TECH
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