Preparation method of ordered porous nerve conduit material

A nerve conduit and composite material technology, applied in the field of nerve conduit materials, can solve problems such as uneven mechanical strength, compactness, impact on catheter implantation and surgery, and achieve good biocompatibility, enhanced mechanical properties, and cross-linking conditions mild effect

Inactive Publication Date: 2020-05-22
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Electrospinning is a simple, low-cost, and efficient method for preparing nerve conduit materials, but the mechanical strength of the prepared n...

Method used

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  • Preparation method of ordered porous nerve conduit material
  • Preparation method of ordered porous nerve conduit material
  • Preparation method of ordered porous nerve conduit material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, preparing a nerve guide material, comprises the following steps:

[0032] The first step: synthesis of catechol-modified chitosan,

[0033] At room temperature, dissolve 0.5 g chitosan (CHI) in 100 mL of 0.05 mol / L HCl solution and stir overnight to obtain a 1 wt.% CHI solution; add 0.1 mol / L NaOH solution dropwise to CHI In the solution, adjust the pH of the solution to 4.5, then add 0.4g 3,4-dihydroxyphenylpropionic acid (catechol, HCA) to the solution, and stir until it becomes clear to obtain solution A; at the same time, add 0.48g 1-ethyl -(3-Dimethylaminopropyl)carbodiimide hydrochloride (EDC) was dissolved in 50 mL of 50vol.% ethanol solution to obtain solution B, and solution B was added dropwise to solution A, at 30 React at ℃ for 12 hours. After the reaction, dialyze the reaction solution in a pH=5.0 HCl solution containing NaCl (1wt.%) for 48 hours, change the dialysate every 12 hours, and then dialyze in deionized water for 24 hours , the rea...

Embodiment 2

[0039] Example 2, using the nerve guide material prepared above for cell migration:

[0040] Soak the nerve guide material in 75vol.% ethanol, irradiate with ultraviolet light for 30 minutes to disinfect, then wash with PBS solution 3 times to remove ethanol, soak the obtained nerve guide material in PC12 basal medium, and make the medium submerge the nerve guide 2 / 3 of the material height, add 20 μL containing 10 5 PC12 cell fluid, placed in 5% CO 2 The concentrations were cultured in a 37°C incubator for 3 hours, 6 hours, 12 hours and 24 hours to observe the cell proliferation and migration, such as Figure 7 , is the value-added and migration fluorescence map of PC12 cells on the ordered porous nerve guide material at different times (3 hours, 6 hours, 12 hours and 24 hours). The results show that PC12 cells can migrate from the top to the bottom of the nerve guide material, and can Proliferate effectively.

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Abstract

The invention discloses a preparation method of an ordered porous nerve conduit material. Aiming at the difficulties of low mechanical strength, non-uniformity, difficulty in organic solvent removal and the like of materials in preparation of different nerve conduit materials, the invention prepares the ordered porous catechol-modified chitosan nerve conduit three-dimensional scaffold material bya directional freeze-drying means. By doping graphene oxide, the nerve conduit material has certain conductivity, and meanwhile, the mechanical property of the material is enhanced, so that the high-strength ordered porous nerve conduit three-dimensional scaffold material is formed. Meanwhile, highly-differentiated pheochromocytoma cells (PC12) are used for monitoring the cell migration and proliferation process, and a way is provided for material selection of nerve conduit transplantation and nerve regeneration medical application.

Description

technical field [0001] The invention relates to the related technical field of nerve guide materials, in particular to a preparation method of ordered porous nerve guide materials. Background technique [0002] Peripheral nerve injury has become a common disease, affecting more than 1 million people worldwide each year. Regeneration and repair after nerve injury has become one of the major problems in the current medical field. With the development of microsurgical equipment and technology, the clinical efficacy of peripheral nerve injury repair has been continuously improved. Autologous transplantation is still the gold standard for clinical peripheral nerve injury repair today, but its source is limited, and it needs to be obtained from other parts of the body. The patient causes secondary harm. At present, with the development of tissue engineering, the construction of nerve conduits with the basic methods and principles of tissue engineering has become a research hot...

Claims

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Application Information

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IPC IPC(8): A61L27/02A61L27/20A61L27/18A61L27/50A61L27/56C08B37/08
CPCA61L27/20A61L27/18A61L27/025A61L27/56A61L27/50C08B37/003A61L2430/32
Inventor 曹一平雷杨吕锐张玮莹
Owner JIANGHAN UNIVERSITY
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