Traditional Chinese medicinal material bio-based carbon nanodot, preparation method thereof, fluorescent probe, traditional Chinese medicinal material medicinal preparation and application

A technology of bio-based carbon and carbon nano-dots, applied in nano-optics, drug combination, nano-technology, etc., can solve the difficulty of reducing C-dots, increasing the stability of the subsequent system and the maintenance of subsequent effects, increasing the design and research Difficulty and cost issues, to achieve the effect of stable optical performance, stable fluorescence excitation-dependent characteristics, and suitable particle size

Active Publication Date: 2020-10-16
CHANGCHUN UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0006] However, this kind of biological functional system is composed of C-dots with unique fluorescent properties and functional substances through multiple ways. Such a complex structure not only increases the difficulty and cost of design and research, but also increases the This reduces the advantages of ultra-small size C-dots in terms of water solubility and biocompatibility, and increases the difficulty of maintaining the stability of the subsequent system and subsequent actions

Method used

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  • Traditional Chinese medicinal material bio-based carbon nanodot, preparation method thereof, fluorescent probe, traditional Chinese medicinal material medicinal preparation and application
  • Traditional Chinese medicinal material bio-based carbon nanodot, preparation method thereof, fluorescent probe, traditional Chinese medicinal material medicinal preparation and application
  • Traditional Chinese medicinal material bio-based carbon nanodot, preparation method thereof, fluorescent probe, traditional Chinese medicinal material medicinal preparation and application

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preparation example Construction

[0070] The present invention also provides a preparation method of bio-based carbon nano-dots, comprising the following steps:

[0071] 1) After mixing total ginsenosides with water, a mixed solution is obtained;

[0072] 2) After the mixed solution obtained in the above steps is subjected to a hydrothermal reaction, an aqueous solution of carbon dots of total ginsenosides is obtained.

[0073] In the present invention, the selection and composition of the raw materials in the preparation method of the bio-based carbon nano-dots, as well as the corresponding optimization principles, can be compared with the selection and composition of the raw materials corresponding to the aforementioned bio-based carbon nano-dots, and the corresponding optimization principles. Correspondingly, details are not repeated here.

[0074] In the invention, firstly, the total ginsenosides are mixed with water to obtain a mixed solution.

[0075] In principle, the present invention has no special ...

Embodiment 1

[0097] Use an electronic balance to weigh 0.5 g of total ginsenosides, dissolve in 10 mL of deionized water, and stir magnetically to fully dissolve it. Then, transfer the solution to a polytetrafluoroethylene high-pressure reaction kettle, tighten the lid of the kettle, control different reaction temperatures (170-200°C) and different reaction times (2-10h), close the oven after the reaction, cool to room temperature, and The obtained liquid is filtered to remove large precipitates and agglomerates to obtain a total ginsenoside carbon point solution, which can be stored in a refrigerator at 4°C until use.

[0098] The preparation of total ginseng saponins carbon dot solution was characterized.

[0099] When the reaction temperature is 200°C, react for 2 to 10 hours, the bottom sediment of the reaction kettle is black, and the total ginsenoside carbon dot solution is a light yellow transparent liquid, and with the increase of the reaction time, the color of the solution gradua...

Embodiment 2

[0115] The preparation steps were the same as in Example 1. The reaction conditions were hydrothermal conditions of 170° C. and 6 h, and the preparation experiment was carried out by changing the reaction amount of total ginsenosides (0.5 g, 1.0 g and 2.0 g).

[0116] The carbon points of total ginsenosides prepared in Example 2 of the present invention were detected.

[0117] see Figure 4 , Figure 4 Fluorescence spectra corresponding to different total saponin reaction amounts of ginseng total saponins carbon points prepared in Example 2 of the present invention. Among them, a) the fluorescence spectrum of the carbon dot solution prepared for 0.5g ginsenosides; b) the fluorescence spectrum of the carbon dot solution prepared for 1g of ginsenosides; c) the fluorescence spectrum of the carbon dot solution prepared for 2g of ginsenosides.

[0118] Depend on Figure 4 It can be seen that the fluorescence emission wavelengths of the three groups of solutions all have the fluo...

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Abstract

The invention provides a traditional Chinese medicinal material bio-based carbon nanodot. The bio-based carbon nanodot is a ginseng total saponin carbon nanodot. The carbon dot surface of the total ginsenoside is rich in alcohol/phenolic hydroxyl, amino, carboxyl and other structures, the surface groups are rich, subsequent modification and compounding are facilitated, and the total ginsenoside has good time, pH value and salt stability; various cell fluorescence imaging experiments prove that the total ginsenoside carbon dots can be used as a biological fluorescence probe; meanwhile, the obtained total ginsenoside carbon dots have a specific inhibition effect on rat adrenal pheochromocytoma cells (PC12 cells), and have no obvious proliferation or inhibition effect on other cells (HeLa cells, BRL3A cells and LO2 cells); meanwhile, the total ginsenoside carbon dots can be used as fluorescent probes and can be taken by various cells (BRL3A cells, LO2 cells and QGY7703 cells).

Description

technical field [0001] The present invention relates to the technical field of bio-based carbon nano-dots, in particular to a bio-based carbon nano-dot and a preparation method thereof, a fluorescent probe, a pharmaceutical preparation, and an application, especially to a Chinese medicinal material bio-based carbon nano-dot and a preparation method thereof, Bio-based fluorescent probes, bio-based pharmaceutical preparations from Chinese herbal medicines, and applications. Background technique [0002] As a plant-derived Chinese herbal medicine, ginseng has enjoyed the reputation of "the king of all herbs" since ancient times. Modern clinical evidence shows that ginseng has a variety of therapeutic effects, mainly due to the fact that ginseng contains a large number of various biologically active substances, such as ginsenosides, volatile oils, polysaccharides, etc. Among them, ginsenoside is the most important bioactive substance in ginseng. Many studies have proved that it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00A61K36/258A61P35/00G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00A61K36/258A61P35/00G01N21/6428G01N21/6458G01N2021/6439G01N2021/6417Y02A50/30
Inventor 姜英男肖立智张阳闫茹月庞博连文慧孙天霞张哲
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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