Bifidobacterium longum subsp. infantis ccfm687, its fermented food and its application
A technology of bifidobacterium longum and fermented food, applied in the field of microbiology, can solve the problems of reduced synthesis and secretion of 5-HT, decreased compliance of patients taking medicine, and no preventive effect, so as to improve intestinal flora disorder and increase secretion The effect of increasing the amount and increasing the abundance
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Embodiment 1
[0068] Example 1: Bifidobacterium longum infantile subspecies CCFM687 can significantly improve the behavioral indicators of depressed mice
[0069] Thirty-two 6-week-old male C57BL / 6J mice were randomly divided into four groups according to body weight after one week of adaptation: control group, model group, fluoxetine intervention group, and CCFM687 intervention group, with 8 mice in each group. The animal groups and treatment methods are shown in Table 1.
[0070] Table 1 Grouping and processing methods of animal experiments
[0071]
[0072] Chronic unpredictable stress depression mouse model: 1-2 kinds of stimuli are randomly used every day, and the time of stimulation is randomly determined every day to avoid circadian rhythm. No more than three times of each method for a period of five weeks. Stimulus factors include: (1) fasting for 24 hours; (2) water deprivation + empty bottle stimulation for 24 hours; (3) tail clamping for 3 minutes; (4) wet litter for 24 hour...
Embodiment 2
[0083] Example 2: CCFM687 can significantly increase the levels of neurotransmitters and precursors in the brains of depressed mice
[0084] The mice in Example 2 were euthanized at the end of the sixth week, and the brain tissues of the mice were taken, and the hippocampus and prefrontal cortex were separated on ice. Take a certain quality of fresh hippocampus and prefrontal cortex tissue (weight not less than 50mg), add 9 times the volume of sterile PBS buffer (equivalent to 1g tissue plus 9ml homogenate), and homogenize with a tissue homogenizer , the tissue fluid was centrifuged at 3000 g for 20 min, and the supernatant was taken, and the contents of 5-HT and BDNF were detected with an ELISA kit. Add an equal volume of 5% perchloric acid to precipitate protein in the supernatant of hippocampal tissue fluid, centrifuge at 10,000 g for 10 min, absorb the supernatant and filter it through a 0.22 μm water filter membrane, and perform 5- Determination of HTP content. Shimadzu...
Embodiment 3
[0085] Example 3: Bifidobacterium longum infantile subspecies CCFM687 can increase the content of butyric acid in the intestinal tract of mice
[0086] The mice in Example 2 were euthanized at the end of the sixth week, and the contents of the cecum of the mice were collected. Weigh 50mg of cecal contents after vacuum freeze-drying into EP tube, add 500uL saturated NaCl, shake evenly; add 40uL 10% sulfuric acid, shake evenly; add 1ml ether, shake evenly; Clear to 2mlEP tube, add 0.25g anhydrous sodium sulfate. Centrifuge at 18000g for 15min at 4°C. Take 500uL of the supernatant to a gas-phase vial, and detect the content of short-chain fatty acids (acetic acid, propionic acid, isobutyric acid, butyric acid, and isovaleric acid) by GC-MS. GC-MS adopts Rtx-Wax column, the column length is 30m, the inner diameter is 0.25μm; the carrier gas is He, the flow rate is 2mL / min; the injection volume is 1μL, the split ratio is 10:1; the injection temperature is set at 240℃, The temper...
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