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Bifidobacterium bifidum BXM0 and application thereof

A technology of bifidobacteria bifidum and bacterial agent, applied to bifidobacteria bifidum BXM0 and its application field, can solve problems such as inability to be widely used, large side effects and the like

Active Publication Date: 2021-12-24
BEIJING QUANTIHEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of these drugs will cause relatively large side effects, so these drugs are only limited to some groups of patients with severe conditions. For the current huge depressed population, this drug treatment cannot be widely used

Method used

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  • Bifidobacterium bifidum BXM0 and application thereof
  • Bifidobacterium bifidum BXM0 and application thereof
  • Bifidobacterium bifidum BXM0 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: the preparation of the identification of Bifidobacterium bifidum BXM0

[0050] 1.1 Identification of Bifidobacterium bifidum BXM0 strain

[0051] The feces samples of healthy adults living in the urban area of ​​Beijing for a long time were collected, diluted and spread on YCFA medium, cultured anaerobically at 37°C for 24-48 hours, and isolated different single colonies on the plate. Use a sterilized inoculation loop to pick different single colonies and streak them on a new YCFA solid medium plate for purification, and culture them anaerobically at 37°C for 48 hours to obtain purified single colonies. Each purified single colony was spread on a mass spectrometer plate, and the lysate and matrix were added respectively. After drying, mass spectrometry was carried out on a MALDI-TOF MS 1000 mass spectrometer (Autobio, Zhengzhou Antu Biotechnology Co., Ltd.). After the identification was completed, each strain was frozen at -80°C in the strain resource ban...

Embodiment 2

[0054] Embodiment 2: paper chromatography detects GABA

[0055] Take 1 mL of Bifidobacterium bifidum BXM0 fermentation broth and centrifuge at 12,000 rpm for 10 min at 4°C to remove the bacteria, and transfer the supernatant to a new centrifuge tube for future use. Pipette 4 μL of sample supernatant (set for 2 repetitions), corresponding culture medium and any concentration of GABA standard solution (0.2, 0.4, 0.8, 1.2, 1.6 and 2.0 g / L) to spot on the filter paper, the spot line distance The edge of the chromatography paper is 2.0 cm, and the spacing between samples is 2.0 cm. After the sample spot is dried, put the chromatographic paper in the developer (V (n-butanol): V (glacial acetic acid): V (water) = 5: 3: 2, the amount of ninhydrin is 1.2% (w / v)) Develop in medium for 50 min (when the developer is about 1 cm away from the upper edge of the chromatography paper). Immediately after the chromatography, the chromatography paper was dried in an oven at 90°C for 30 min to d...

Embodiment 3

[0059] Embodiment 3: High performance liquid chromatography HPLC method detects bacterial liquid GABA

[0060] Accurately weigh GABA, prepare GABA standard solutions with mass concentrations of 0.04, 0.08, 0.12, 0.16 and 0.20 g / L, take 100 μL of GABA standard solutions of each concentration, and add 100 μL of OPA (phthalaldehyde) derivative Mix well and incubate at room temperature for at least 90s. After the reaction, 20 μL of each solution was injected, and the peak absorption was measured at 338 nm. The standard curve of GABA concentration detection was established by the peak area. Then, add OPA (phthalaldehyde) derivative to the diluted BXM0 supernatant, incubate and mix well, inject the sample, and measure the absorption peak at 338 nm.

[0061] Bifidobacterium bifidum BXM0 was cultured in YCFA liquid medium (without additional L-glutamic acid) for 24 hours, and the amount of GABA that could be produced by HPLC was 0.273g / L. It is worth mentioning that various concent...

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Abstract

The invention belongs to the field of microorganisms and application thereof, and provides Bifidobacterium bifidum BXM0, and the collection number of the Bifidobacterium bifidum BXM0 is CGMCC No. 22146. The invention also provides application of the Bifidobacterium bifidum BXM0 in production of GABA and the prevention and / or treatment of depression. The Bifidobacterium bifidum BXM0 provided by the invention has a good GABA production capacity, can obviously relieve the depression-like behaviors of depression model mice, provides a good scientific basis for developing effective anti-depression probiotic products in the future, and has a great potential market value.

Description

technical field [0001] The invention belongs to the field of microorganisms and their applications, in particular to Bifidobacterium bifidum BXM0 and their applications. Background technique [0002] The pace of work and life in modern cities is accelerating, and tension and pressure are constantly flooding each of us every day. Irregular work and rest, aging, decline in metabolic immunity and other internal pressures are constantly changing. Insomnia, anxiety and sub-health have become labels for many people. Accumulated stress over time can lead to insomnia, lack of energy, gastrointestinal disorders, and even depression. According to the latest statistics from the World Health Organization (WHO) in 2019, there are more than 350 million depressed patients worldwide. As of 2019, the number of depressed people in China has exceeded 95 million. Listless, severe cases of insomnia, loss of interest in life, suicide, and suicide. Depression is expected to become the world's nu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61P25/24C12R1/01
CPCC12N1/20A61K35/745A61P25/24
Inventor 徐文艺王情世李转羽周雨柠赵柏闻
Owner BEIJING QUANTIHEALTH TECH CO LTD
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