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Bifidobacterium longum subsp. infantis CCFM687 as well as fermented food and application thereof

A technology of Bifidobacterium longum and fermented food, applied in the field of microorganisms, can solve the problems of reduced 5-HT synthesis and secretion, reduced patient compliance with medication, and no preventive effect, so as to improve intestinal flora disturbance and increase secretion quantity, the effect of increasing abundance

Active Publication Date: 2018-12-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the drug has the following two problems: first, there is a delay of 2-4 weeks in the onset of the drug, because after taking SSRI to increase the concentration of 5-HT in the synaptic cleft, 5-HT will react on the presynaptic 5-HT1A receptor body, resulting in negative feedback inhibition, leading to a decrease in the synthesis and secretion of presynaptic 5-HT
And these 2-4 weeks will lead to a decrease in patients' medication compliance in clinical practice and greatly increase the risk of suicide
Second, most antidepressants do not have a preventive effect, and long-term medication can cause side effects such as non-specific abdominal pain, constipation, diarrhea, indigestion, and flatulence

Method used

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  • Bifidobacterium longum subsp. infantis CCFM687 as well as fermented food and application thereof
  • Bifidobacterium longum subsp. infantis CCFM687 as well as fermented food and application thereof
  • Bifidobacterium longum subsp. infantis CCFM687 as well as fermented food and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Bifidobacterium longum infantile subspecies CCFM687 can significantly improve the behavioral indicators of depressed mice

[0069] Thirty-two 6-week-old male C57BL / 6J mice were randomly divided into four groups according to body weight after one week of adaptation: control group, model group, fluoxetine intervention group, and CCFM687 intervention group, with 8 mice in each group. The animal groups and treatment methods are shown in Table 1.

[0070] Table 1 Grouping and processing methods of animal experiments

[0071]

[0072] Chronic unpredictable stress depression mouse model: 1-2 kinds of stimuli are randomly used every day, and the time of stimulation is randomly determined every day to avoid circadian rhythm. No more than three times of each method for a period of five weeks. Stimulus factors include: (1) fasting for 24 hours; (2) water deprivation + empty bottle stimulation for 24 hours; (3) tail clamping for 3 minutes; (4) wet litter for 24 hour...

Embodiment 2

[0083] Example 2: CCFM687 can significantly increase the levels of neurotransmitters and precursors in the brains of depressed mice

[0084] The mice in Example 2 were euthanized at the end of the sixth week, and the brain tissues of the mice were taken, and the hippocampus and prefrontal cortex were separated on ice. Take a certain quality of fresh hippocampus and prefrontal cortex tissue (weight not less than 50mg), add 9 times the volume of sterile PBS buffer (equivalent to 1g tissue plus 9ml homogenate), and homogenize with a tissue homogenizer , The tissue fluid was centrifuged at 3000g for 20min, and the supernatant was taken, and the content of 5-HT and BDNF was detected by ELISA kit. Add an equal volume of 5% perchloric acid to precipitate protein in the supernatant of hippocampal tissue fluid, centrifuge at 10,000 g for 10 min, absorb the supernatant and filter it through a 0.22 μm water filter membrane, and perform 5- Determination of HTP content. Shimadzu Intertsi...

Embodiment 3

[0085] Example 3: Bifidobacterium longum infantile subspecies CCFM687 can increase the content of butyric acid in the intestinal tract of mice

[0086] The mice in Example 2 were euthanized at the end of the sixth week, and the contents of the cecum of the mice were collected. Weigh 50 mg of cecal contents after vacuum freeze-drying into EP tube, add 500uL saturated NaCl, shake evenly; add 40uL 10% sulfuric acid, shake evenly; add 1ml ether, shake evenly; Clear to 2mlEP tube, add 0.25g anhydrous sodium sulfate. Centrifuge at 18000g for 15min at 4°C. Take 500uL of the supernatant to a gas-phase vial, and detect the content of short-chain fatty acids (acetic acid, propionic acid, isobutyric acid, butyric acid, and isovaleric acid) by GC-MS. GC-MS adopts Rtx-Wax column, the column length is 30m, the inner diameter is 0.25μm; the carrier gas is He, the flow rate is 2mL / min; the injection volume is 1μL, the split ratio is 10:1; the injection temperature is set at 240℃, The tempe...

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Abstract

The invention relates to bifidobacterium longum subsp. infantis CCFM687 as well as fermented food and application thereof. The bifidobacterium longum subsp. infantis CCFM687 provided by the inventioncan improve the depressive behavior of a depression mouse and raise the level of 5-hydroxytryptamine, 5-hydroxytryptophane and brain-derived neurotrophic factor in the brain of the depression mouse; moreover, the content of butyric acid in the intestines of the depression mouse is increased; the abundance of intestinal desulfovibrio is lowered while the abundance of bifidobacterium and S24-7 family is raised, the intestinal flora alpha-diversity is improved, the intestinal flora disturbance of the depression mouse is relieved, and the occurrence of autism, inflammatory bowel disease, obesity,diabetes mellitus type I and the like is reduced; the level of mRNA simulating tryptophan hydroxylase in enterochromaffin cell is raised, the secretion of 5-hydroxytryptophane of the enterochromaffincell is increased, and a precursor substance is provided for the synthesis of 5-hydroxytryptamine in brain. Therefore, the bifidobacterium longum subsp. infantis CCFM687 has a broad application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Bifidobacterium longum subspecies CCFM687, fermented food and applications thereof. Background technique [0002] Depression, also known as depressive disorder, is characterized by significant and persistent depression as the main clinical feature. Depression is actually an umbrella term that includes major depressive disorder, bipolar disorder, seasonal affective disorder, postpartum depression, and more. Currently, more than 350 million people worldwide suffer from depression, and it is estimated that by 2030, depression will rank first in the global disease burden. [0003] Depression is a heterogeneous disorder with inconsistencies in response to treatment. The pathogenesis of depression is not yet clear, and the imbalance of monoamine neurotransmitters is the main research direction, which has also been confirmed by a large number of cases. The main fea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23L29/00A61K35/745A61P25/24A61P25/00A61P29/00A61P1/00A61P3/04A61P3/10C12R1/01
CPCA61P1/00A61P3/04A61P3/10A61P25/00A61P25/24A61P29/00A23L29/065A23L33/135A23V2002/00A61K2035/115C12R2001/01C12N1/205A23V2400/533A23V2200/328A23V2200/332A23V2200/322A23V2200/32
Inventor 陈卫王刚田培郡赵建新张灏
Owner JIANGNAN UNIV
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