1results about How to "Reduce feedback inhibition" patented technology

This application relates to the fields of genetic engineering and enzyme engineering, specifically to an ornithine decarboxylase mutant, gene, recombinant plasmid, recombinant strain, and its applications. The ornithine decarboxylase mutant is obtained by mutating the wild-type ornithine decarboxylase with the amino acid sequence shown in SEQ ID NO.1, and the mutation sites of the ornithine decarboxylase mutant include any one or more of Y104F, A264V, Q263E, R157K, S325C, and V265A. By performing single-point or multi-site combination mutations on the wild-type ornithine decarboxylase, a high-activity ornithine decarboxylase mutant is obtained, thereby significantly improving the substrate conversion rate of the ornithine decarboxylation reaction and the synthesis efficiency of butanediamine. This successfully overcomes the rate-limiting bottleneck of the ornithine decarboxylation reaction in the synthetic pathway from glucose to ornithine and then to butanediamine, significantly optimizing the overall efficiency of this metabolic pathway.