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Hydrogen manufacturing method for high-density culture and hydrogen production integration of platymanas subcordiformis

A technology for high-density cultivation of P. subcordiformis, which is applied in the field of hydrogen production integrating high-density cultivation and hydrogen production of P. subcordiformis. Destruction and other problems, to achieve the effect of increasing hydrogen production, eliminating product inhibition, and reducing manpower input

Inactive Publication Date: 2010-06-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

The hydrogen production process is complicated, and centrifugal concentration requires additional energy consumption, and will cause certain damage to microalgae cells, thereby reducing hydrogen production capacity.
However, there is no report on the technology of combining high-density culture and hydrogen production process with a flat-panel photobioreactor integrated with a fuel cell.

Method used

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  • Hydrogen manufacturing method for high-density culture and hydrogen production integration of platymanas subcordiformis
  • Hydrogen manufacturing method for high-density culture and hydrogen production integration of platymanas subcordiformis
  • Hydrogen manufacturing method for high-density culture and hydrogen production integration of platymanas subcordiformis

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Embodiment 1

[0042] The reactor used is as figure 1 As shown, it is made of 3mm flat glass, the specification is 20cm*20cm*3cm, and the actual use volume is 1.5L. Platymonas subcordiformis required for the experiment was purified in the laboratory, cultured in a 2-liter Erlenmeyer flask with Kangweifang nutrient salt as the medium, and the initial inoculation density was 5×10 4 cell / mL, the volume of algae liquid is 1 liter; the culture temperature is 25°C; the light intensity is 50μE / m 2 .s -1 ; The light-to-dark ratio is 14:10. When the algae in shake flasks are in exponential growth phase, dilute to 10 6 cell / mL, inoculated in a plate reactor, the volume of algae liquid is 1.5L. During the cultivation process, different concentrations of CO 2 The algae liquid was aerated to investigate different concentrations of CO 2 Effects on the growth of flat algae, and find out the CO suitable for high-density cultivation of flat algae 2 concentration. Experimental results such as figure...

Embodiment 2

[0045] exist figure 1 In the reactor shown, when the algae density reaches 8×10 6 When cell / mL, close the CO 2 Cylinders, and keep the reactor dark. Then turn the gas conversion valve to N 2 Air valve, and open the nitrogen bottle, use nitrogen to purge the oxygen in the reactor, and the anaerobic state will be reached in the reactor after 10 minutes. Finally, the gas path switching valve was closed, and the reactor was placed in a dark place for 4 hours of dark induction. During this period, the micro-vacuum circulation pump should always be in an open state to stir the algae liquid. Before the light, inject 15 μL of CCCP into the reactor, and then carry out continuous light to produce hydrogen after 20 minutes, and the light intensity is 100 μE / m 2 .s -1 .

[0046] Figure 6 The results showed that the rapid light-induced curve decreased continuously with the passage of hydrogen production time, indicating that the ability of photosynthetic electron transfer (relativ...

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Abstract

The invention utilizes a flat plate photobioreactor integrating a fuel battery to integrate microalgae high-density culture and irradiation hydrogen production so as to form an integrative hydrogen production system. Two sections of processes of microalgae culture and photosynthetic hydrogen production are generally divided in space time, and the cultured low-density frustule needs to be concentrated and then irradiated to produce hydrogen, so that the operations need to be respectively carried out in two photobioreactors. In the microalgae hydrogen production of the invention, the low-density microalgae is inoculated into a reactor, a culture mode of enriching carbon dioxide is utilized, the biomass required by the hydrogen production can be reached by short-term culture, then dark induction is carried out, and the operation can enter a continuous irradiation hydrogen production stage. The generated hydrogen gas can be integrated into a fuel battery on a reactor and is converted into electric energy, and the hydrogen production condition can be monitored at real time by the change of current. The invention combines the two sections of processes of the microalgae culture and the hydrogen production for the first time, utilizes the fuel battery to convert the hydrogen into the electric energy and provides reliable technical support for the large-scale microalgae hydrogen production.

Description

technical field [0001] The invention relates to a new method for producing hydrogen by using marine microalgae. That is: a new process of high-density cultivation and hydrogen production integration. technical background [0002] With the in-depth research on hydrogen production by green algae in recent years, people have discovered many kinds of green algae with obvious hydrogen production capacity, such as: Scenedesmus obliquus, Chlamydomonas reinhardtii, Platymonas subcordiformis, etc. At the same time, the research on hydrogen production process has also made a lot of fruitful progress. For example, Zhang et al. found that under continuous light conditions of sulfur deficiency in freshwater Chlamydomonas reinhardtii, due to the lack of sulfur, the synthesis of cysteine ​​was blocked, which led to the failure of the D1 protein on PS II to be repaired in time, reducing the photochemical activity and Oxygen production. When the rate of oxygen production in the system is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12N1/12C12R1/89
Inventor 张卫郑阳陈兆安陆洪斌彦飞傅赟彬
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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