Method for fermentation production of bacitracin

A technology of bacitracin and fermentation liquid, which is applied in the field of fermentation engineering, can solve the problems of unstable fermentation process and low fermentation level, and achieve the effects of increasing bacitracin production, increasing production, and increasing production capacity

Inactive Publication Date: 2016-04-13
NCPC NEW DRUG RES & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the problem of unstable fermentation process and low fermentation level in the preparation of bacitracin by microbial fermentation in the prior art, and to provide a method for improving the yield of bacitracin by optimizing the fermentation medium formula and fermentation conditions

Method used

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  • Method for fermentation production of bacitracin
  • Method for fermentation production of bacitracin
  • Method for fermentation production of bacitracin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、500

[0029] Embodiment 1, 500mL shake flask fermentation prepares bacitracin

[0030] Preparation of Seed Solution of Bacillus licheniformis Strain

[0031] The slant culture of the Bacillus licheniformis strain was inoculated into the seed medium, and cultured at 28° C. and 240 rpm for 24 hours to obtain a seed liquid.

[0032] The seed medium described therein is obtained by the following method: 3.0 grams of cornstarch, 20.0 grams of glucose, 20.0 grams of yeast powder, 1.0 grams of ammonium sulfate, 2.0 grams of sodium chloride, 1.0 grams of magnesium sulfate heptahydrate, add tap water to be constant to 1000mL, pH6.5, sterilized at 121°C for 30min.

[0033] The bacitracin producing bacterium of the present invention is the strain numbered ATCC10716.

[0034] Fermentation medium composition:

[0035] 30.0 grams of corn starch, 20.0 grams of glucose, 30.0 grams of cottonseed protein powder, 10.0 grams of yeast powder, 3.0 grams of ammonium sulfate, 5.0 grams of corn steep liq...

Embodiment 2、5

[0038] Embodiment 2, 5L tank fermentation prepares bacitracin

[0039] Preparation of Seed Solution of Bacillus licheniformis Strain

[0040] The slant culture of the Bacillus licheniformis strain was inoculated into the seed medium, and cultured at 30° C. and 200 rpm for 20 hours to obtain a seed liquid.

[0041] The seed medium described therein is obtained by the following method: 5.0 grams of cornstarch, 10.0 grams of glucose, 10.0 grams of yeast powder, 2.0 grams of ammonium sulfate, 2.0 grams of sodium chloride, 1.5 grams of magnesium sulfate heptahydrate, add tap water to be constant to 1000mL, pH7.0, sterilized at 121°C for 30min.

[0042] The bacitracin producing bacterium of the present invention is the strain numbered ATCC10716.

[0043] Fermentation medium composition:

[0044] 90.0 grams of corn starch, 60.0 grams of glucose, 45.0 grams of cottonseed protein powder, 45.0 grams of yeast powder, 6.0 grams of ammonium sulfate, 12.0 grams of corn steep liquor, 4.5 ...

Embodiment 3、10

[0047] Embodiment 3, 10L tank fermentation prepares bacitracin

[0048] Preparation of Seed Solution of Bacillus licheniformis Strain

[0049] The slant culture of the Bacillus licheniformis strain was inoculated on the seed medium, and cultured at 28° C. and 220 rpm for 28 hours to obtain a seed solution.

[0050] The seed medium described therein is obtained by the following method: 4.0 grams of cornstarch, 15.0 grams of glucose, 15.0 grams of yeast powder, 1.0 grams of ammonium sulfate, 1.0 grams of sodium chloride, 1.0 grams of magnesium sulfate heptahydrate, add tap water to be constant to 1000mL, pH6.8, sterilized at 121°C for 30min.

[0051] The bacitracin producing bacterium of the present invention is the strain numbered ATCC10716.

[0052] Fermentation medium composition:

[0053] 200.0 grams of corn starch, 50.0 grams of glucose, 150.0 grams of cottonseed protein powder, 100.0 grams of yeast powder, 10.0 grams of ammonium sulfate, 15.0 grams of corn steep liquor,...

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Abstract

The invention belongs to the field of fermentation engineering, and particularly relates to a method for improving the yield of bacitracin produced through microbial fermentation. According to the method, by optimizing an organic composite nitrogen source and fermentation conditions, batch fermentation unit stability is ensured, and the content of impurities F is lowered; superparamagnetism microspheres are added in the bacitracin synthesis period to adsorb bacitracin in a fermentation solution, and therefore feedback restraint of a fermentation production for producing bacteria is lowered. After fermentation is finished, the fermentation unit of bacitracin can reach 1228 U / ml, the yield of bacitracin can be improved by 123% compared with the yield obtained before optimization, the yield is improved, energy consumption is lowered, and waste discharge is reduced.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a method for producing bacitracin by fermentation. Background technique [0002] Bacitracin is a thiazole peptide narrow-spectrum antibiotic composed of 12 amino acids, mainly produced by fermentation of Bacillus subtilis treschii and Bacillus licheniformis, effective against gram-positive cocci and bacilli, and a small number of gram-negative bacteria , Spirochetes and actinomycetes also have a certain inhibitory effect. Bacitracin has the advantages of high efficiency, low toxicity, and less residue. Therefore, bacitracin is not only an important antibiotic drug, but also a safe antibiotic feed additive for livestock and poultry. [0003] Bacitracin is a mixture of several similar polypeptide components, containing isomers with similar chemical structures such as Bacitracin A, B1, B2, B3, C1, C2, C3, E, F, etc. The main active ingredient is Bacitracin A, M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/00C12R1/10
CPCC07K7/58C12N1/20C12P21/00C12P21/02
Inventor 任风芝张雪霞王耀耀耿文飞郑智慧杨海静高任龙段宝玲张炜
Owner NCPC NEW DRUG RES & DEV
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