34results about "Bacitracins" patented technology

The invention belongs to the field of fermentation engineering, and particularly relates to a method for improving yield of bacitracin by microbial fermentation. According to the method, by a step 1) of optimization of organic compounding of a nitrogen source and fermentation conditions, stability of fermentation units between batches is ensured, and the content of impurities F is reduced; and by a step 2) of adding superparamagnetic microspheres during the synthesis of bacitracin, feedback inhibition of produced bacteria by fermentation products is reduced. After fermentation, the fermentation unit of bacitracin can reach 1228U / ml, which can increase the yield of bacitracin by 123%, improve the productivity and reduce energy consumption and waste discharge.

The present invention provides an enhanced ppc Expressed Bacillus licheniformis and preparation method and application, wherein the strengthening ppc Bacillus licheniformis was expressed by inserting the phosphoenolpyruvate carboxylase gene derived from Corynebacterium glutamicum ATCC 13032 into the genome of Bacillus licheniformis DW2 ppc The prepared Bacillus licheniformis DW2‑ppc. Compared with Bacillus licheniformis DW2, the bacitracin production of Bacillus licheniformis DW2‑ppc was increased by more than 12%.

The invention provides a preparation method of a bacitracin impurity L based on photocatalysis. The preparation method comprises the following steps: S1, dissolving a bacitracin raw material medicament in a solvent, and performing illuminating at 50-60 DEG C for 1-3h to obtain a crude product containing the bacitracin impurity L; and S2, separating the bacitracin impurity L from other components by adopting a preparation liquid phase, collecting a peak fraction of the bacitracin impurity L, and evaporating the collected peak fraction of the bacitracin impurity L to dryness to obtain a reference product of the bacitracin impurity L. According to the technical scheme of the invention, a photocatalytic chemical conversion method is adopted to convert bacitracin A into an epimer of the bacitracin A, namely an impurity L, under action of illumination, so that the steps are simple and convenient, efficiency is high, low-cost enrichment of an impurity crude product is realized, post-treatmentis simple, cost is low, and pollution is small.

The invention provides a sodium dodecylsulfonate-bacitracin compound and a composite oil displacement agent. The chemical structural formula of the sodium dodecylsulfonate-bacitracin compound is as follows:. The invention provides a sodium dodecylsulfonate-bacitracin compound oil displacement agent containing a sodium dodecylsulfonate-bacitracin compound obtained by reacting sodium dodecylsulfonate and bacitracin. The composite oil displacement agent can be well compounded with oil production functional bacteria to form a microbial-chemical composite oil displacement agent. The microbial-chemical composite oil displacement agent not only has the effect of microbial flooding on crude oil degradation and viscosity reduction, but also has the emulsification and viscosity reduction effect of chemical flooding, and through the biological carrying method, the chemical oil displacement agent can expand the swept volume and dispersion efficiency in the oil layer, Effectively improve the synergistic reaction efficiency of microorganisms and chemical agents to achieve the goal of 1+1>2.

The invention belongs to the field of biotechnology and fermentation engineering, and provides an expression cassette suitable for Bacillus hyaline hemoglobin and its application. The invention combines the signal peptide SPywbN of YwbN in Bacillus subtilis and the promoter of Bacillus subtilis P43 is connected with the amylase terminator TamyL in Bacillus licheniformis to form a VHb protein expression cassette P43‑SPywbN‑ vhb‑ Tamy L. The expression cassette was applied to three kinds of Bacillus (Bacillus subtilis 168, Bacillus amyloliquefaciens LX‑12, Bacillus licheniformis DW2), compared with the conventional single enhanced expression VHb strains, the poly-γ-glutamic acid , Iturin A, and bacitracin yields increased by 21.85%, 18.77% and 23.40%, and the invention provides theoretical guidance for the high-efficiency expression and wide application of Vitiligo hyaline hemoglobin VHb in Bacillus.