34results about "Bacitracins" patented technology

The invention discloses a bacillus licheniformis CPL618 as well as screening and application thereof. The strain CPL618 is identified as bacillus licheniformis, the strain CPL618 is preserved in the China Center for Type Microbiological Culture Collection (CCTCC), the preservation time is September 13, 2021, and the preservation number is CCTCC NO: M20211158. The bacillus licheniformis CPL618 disclosed by the invention has broad-spectrum antibacterial activity, and can antagonize the growth of gram-positive bacteria, gram-negative cocci and fungi. The invention provides an optimized industrial culture medium which can improve the yield of bacitracin A at lower cost, and the maximum yield can reach 2.3 g / L. The bacillus licheniformis disclosed by the invention has a wide utilization prospect in the aspects of preparation of antibacterial agents and production of bacitracin A.

The invention belongs to the field of fermentation engineering, and particularly relates to a method for improving the yield of bacitracin produced through microbial fermentation. According to the method, by optimizing an organic composite nitrogen source and fermentation conditions, batch fermentation unit stability is ensured, and the content of impurities F is lowered; superparamagnetism microspheres are added in the bacitracin synthesis period to adsorb bacitracin in a fermentation solution, and therefore feedback restraint of a fermentation production for producing bacteria is lowered. After fermentation is finished, the fermentation unit of bacitracin can reach 1228 U / ml, the yield of bacitracin can be improved by 123% compared with the yield obtained before optimization, the yield is improved, energy consumption is lowered, and waste discharge is reduced.

The invention provides a low-peculiar smell bacitracin production bacillus licheniformis and a construction method and an application of the low-peculiar smell bacitracin production bacillus licheniformis. A ptb gene in a genomic DNA sequence of the bacillus licheniformis DW2 is knocked out through a genetic engineering method to obtain the bacillus licheniformis DW2deltaptb, and compared with the bacillus licheniformis DW2, the synthetic amounts of isobutyric acid and isovaleric acid of the bacterial strain are respectively reduced to 33% and 37% in the fermentation process of the bacitracin, and the peculiar smell is remarkably reduced.

The invention belongs to the field of fermentation engineering, and particularly relates to a method for improving yield of bacitracin by microbial fermentation. According to the method, by a step 1) of optimization of organic compounding of a nitrogen source and fermentation conditions, stability of fermentation units between batches is ensured, and the content of impurities F is reduced; and by a step 2) of adding superparamagnetic microspheres during the synthesis of bacitracin, feedback inhibition of produced bacteria by fermentation products is reduced. After fermentation, the fermentation unit of bacitracin can reach 1228U / ml, which can increase the yield of bacitracin by 123%, improve the productivity and reduce energy consumption and waste discharge.

The invention relates to the field of microbial engineering and discloses a fermentation medium formula for high production of bacitracin employing bacillus licheniformis and application of the fermentation medium formula. One part of bean pulp is replaced with cheap rapeseed dregs rich in cysteine, so that the fermentation yield of the bacitracin is significantly improved, and the valence of the bacitracin reaches 849.3U / mL and is improved by 12.4% in comparison with that of an original culture medium. Meanwhile, the market price of the bean pulp and the rapeseed dregs is investigated, the price of the bean pulp fluctuates within a small range of about 3200yuan / t, the mean price of the rapeseed dregs fluctuates within a small range of about 2800yuan / t, the fermentation cost is significantly reduced by replacement of fermentation raw materials, and the benefits of the bacitracin fermented by the bacillus licheniformis is improved.

The invention provides bacillus licheniformis for enhancing expression of cysP and a preparation method and application thereof. The bacillus licheniformis is prepared by transferring a plasmid carrier carrying a sulfur element transport protein gene cysP into bacillus licheniformis DW2, thereby realizing free expression of the sulfur element transport protein gene cysP in the bacillus licheniformis DW2, and achieving a purpose of enhancing the expression of the sulfur element transporter gene CysP. Compared with a strain obtained by directly transferring a blank plasmid vector without the cysP gene into the bacillus licheniformis DW2, the bacillus licheniformis for enhancing expression of cysP constructed by the invention has a large increase in bacitracin production.

The invention provides bacillus licheniformis DW2delta recA bacABCs for multiplying the operon bacABC copy number and knocking off a recA gene. The strain is preserved in the China Center for Type Culture Collection in wuhan on June 12th, 2014, and the preservation number is CCTCC NO:M2014251. The genome of the strain also has double characteristics of multiplying the operon bacABC copy number and knocking off the recA gene, and moreover when the bacillus licheniformis DW2delta recA bacABCs for multiplying the operon bacABC copy number and knocking off the recA gene is used in bacitracin production, the yield of bacitracin can be increased by 11.5%.

The invention provides bacillus licheniformis DW2delta recA bacABCs for multiplying the operon bacABC copy number and knocking off a recA gene. The strain is preserved in the China Center for Type Culture Collection in wuhan on June 12th, 2014, and the preservation number is CCTCC NO:M2014251. The genome of the strain also has double characteristics of multiplying the operon bacABC copy number and knocking off the recA gene, and moreover when the bacillus licheniformis DW2delta recA bacABCs for multiplying the operon bacABC copy number and knocking off the recA gene is used in bacitracin production, the yield of bacitracin can be increased by 11.5%.

The invention aims at providing high-purity and high-content zinc bacitracin. A preparation method of the zinc bacitracin comprises the following steps: S1, mixing zinc bacitracin powder and adhesive, adding water for diluting, regulating the pH value to be 5-8, granulating at the temperature of 10-25 DEG C, drying at the temperature ranging from -20 DEG C to 5 DEG C, sieving, and spraying a micro permeable membrane, so as to obtain bacitracin micro capsules; S2, regulating the pH value of an inorganic zinc aqueous solution to be 8-11, gradually adding the prepared bacitracin micro capsules, after salifying, regulating the pH value to be 5-7 with inorganic acid, stirring, and standing; and S3, separating, drying, and smashing, so that the zinc bacitracin is obtained.

The invention provides a method for constructing bacillus licheniformis by knocking out an lrpC gene, a strain and an application of the strain. The bacillus licheniformis DW2 delta lrpC is obtained by knocking out the lrpC gene in a bacillus licheniformis genome with a genetic engineering method, and the yield of bacitracin of the strain in a fermentation liquid in the bacitracin fermentation process is increased by 10% or above.

The invention relates to a method for separating and purifying bacitracin by using a thermo-responsive copolymer NPE-108, which comprises the following steps: a forward extraction step: uniformly mixing NPE-108 and bacitracin fermentation liquor according to a volume ratio of 1: 4, adding divalent manganese salt, adjusting the pH value to 8.5, uniformly oscillating, standing in a constant-temperature water bath kettle at 30 DEG C until two phases with a clear interface is formed, and taking out the upper phase; and a reverse extraction step: adding water of which the volume is half of that of NPE-108 into the upper phase obtained in the forward extraction step, uniformly mixing, adjusting the pH value to 5.5, standing in a water bath kettle at 60 DEG C until two phases with a clear interface are formed, and enriching bacitracin in the upper phase. The thermo-responsive copolymer NPE-108 is mixed with water to form a recoverable thermo-responsive two-aqueous-phase system, and the method has the advantages of mild operation conditions, environmental friendliness and recoverable phase forming components, and is beneficial to large-scale bacitracin separation and application.

The invention discloses bacitracin isolated and purified from a bacitracin fermentation broth and a method for preparing a zinc salt thereof, that is zinc bacitracin. The method includes: adsorbing bacitracin active components in the fermentation broth with super-magnetic microspheres, and preparing high-activity bacitracin via desorption, extraction and precipitation; back extracting an extract into a water phase, reacting with a zinc salt to obtain the zinc bacitracin. The super-magnetic microspheres are highly selective for the bacitracin active components and have weak ability to adsorb impurity bacitracin F, and the introduction of the super-magnetic microsphere medium gives a great simplification in the purifying process and a great increase in yield; the process is high in efficiency, low in energy consumption and low in solvent usage with bacitracin yield up to greater than 70 %, and both the prepared bacitracin and zinc salt comply with the standards of United States Pharmacopoeia.

The invention discloses a cyclic peptide glass and a pharmaceutical composition glass containing a cyclic peptide. The cyclopeptide glass can simultaneously exert the drug effect and the function of a drug auxiliary material, and compared with a crystal and a traditional drug dosage form or an auxiliary material, the cyclic peptide glass can effectively increase the drug dissolution rate and improve the drug bioavailability, and is widely applied to the drug delivery and slow release fields of tumor resistance, virus / bacterium resistance, blood glucose control, immunoregulation, neuroregulation and the like.

The invention provides a sodium dodecyl sulfate-bacitracin compound and a composite oil displacement agent. The chemical structural formula of the sodium dodecyl sulfate-bacitracin compound is shown in the specification. The invention provides a sodium dodecyl sulfate-bacitracin composite oil displacement agent containing a sodium dodecyl sulfate-bacitracin compound obtained by a reaction of sodium dodecyl sulfate and bacitracin. The compound oil-displacing agent can be well compounded with oil recovery functional bacteria to form a microorganism-chemical compound oil displacement agent. The microbial-chemical composite oil displacement agent has the effects of microbial flooding on degradation and viscosity reduction of crude oil as well as chemical flooding on emulsification and viscosity reduction, the swept volume and dispersion efficiency of the chemical oil displacement agent in an oil layer are increased in a biological carrying mode, the efficiency of a synergistic reaction ofmicroorganisms and a chemical agent is effectively improved, and the purpose that one plus one is greater than two is achieved.

The invention discloses a method for producing bacitracin D by fermenting diatomite immobilized bacillus, and belongs to the technical field of biology. According to the method, diatomite is added in the fermentation process of bacillus subtilis fmbJ (CGMCC No.0943) to perform immobilized fermentation on the fmbJ, so that the yield of the secondary metabolite bacitracin D of the bacillus subtilis can be remarkably increased, repeated fermentation can be performed under the condition of not adding any fresh seed solution, and the yield of the bacitracin D is remarkably increased. According to the method, the yield of bacitracin D is increased, the utilization rate of the raw materials is also increased, the production cost is reduced, and a by-product (diatomite adsorbing bacillus subtilis) obtained after fermentation can also be used for preparing a microbial preparation and can be applied to grain storage, fruit and vegetable preservation and animal feed.

The invention provides bacillus licheniformis for enhancing ppc expression, and a preparation method and application thereof. The bacillus licheniformis for enhancing ppc expression is bacillus licheniformis DW2-ppc prepared by inserting phosphoenolpyruvate carboxylase gene ppc derived from corynebacterium glutamicum ATCC 13032 into a genome of bacillus licheniformis DW2. Compared with the bacillus licheniformis DW2, the bacillus licheniformis DW2-ppc has the advantages that the bacitracin yield is improved by 12% or above.

The invention belongs to the field of biotechnology and fermentation engineering. The invention provides a vitreoscilla hemoglobin expression cassette suitable for bacillus and application. Vitreoscilla hemoglobin VHb is connected with a signal peptide SPywbN of YwbN in bacillus subtilis, a bacillus subtilis promoter P43 and an amylase terminator TamyL in bacillus licheniformis to form a VHb protein expression cassette P43-SPywbN-vhb-TamyL. The expression cassette is applied to three types of bacillus subtilis (bacillus subtilis 168, bacillus amyloliquefaciens LX-12 and bacillus licheniformisDW2); compared with a conventional strain for independently and intensively expressing VHb, the yield of poly gamma-glutamic acid, iturin A and bacitracin is increased by 21.85%, 18.77% and 23.40% respectively, and theoretical guidance is provided for efficient expression and wide application of vitreoscilla hemoglobin VHb in bacillus.

The invention discloses a method for improving bacitracin produced by bacillus licheniformis and belongs to the technical field of microorganisms. The bacillus licheniformis ATH with high yield of bacitracin is screened from a feed sample, the acetyl coenzyme A synthetase Bsacs gene derived from bacillus subtilis is expressed in the bacillus licheniformis ATH through a genetic engineering method, supply of amino acid formed by intracellular bacitracin is increased, and the titer of bacitracin is improved. After the obtained recombinant bacillus licheniformis is inoculated into a fermentation culture medium containing soybean meal for fermentation culture for 42 hours, the titer of the obtained bacitracin is 1928 IU / mL and is improved by 46.16% compared with that of the bacitracin of an original strain ATH, and meanwhile, the content of bacitracin A is 1081 IU / mL and is improved by 37.2% compared with that of the original strain. The bacitracin production efficiency in the whole fermentation process is high, the raw material cost is low, and the method is simple and easy to operate.

The present invention provides an enhanced ppc Expressed Bacillus licheniformis and preparation method and application, wherein the strengthening ppc Bacillus licheniformis was expressed by inserting the phosphoenolpyruvate carboxylase gene derived from Corynebacterium glutamicum ATCC 13032 into the genome of Bacillus licheniformis DW2 ppc The prepared Bacillus licheniformis DW2‑ppc. Compared with Bacillus licheniformis DW2, the bacitracin production of Bacillus licheniformis DW2‑ppc was increased by more than 12%.

Provided are malR-knockout Bacillus licheniformis strain, a construction method and a use. Using a genetic engineering method, gene, namely, the malR gene, which is in charge of conducting the transcription of the carbon metabolism transcription factor MalR, in the genome of Bacillus licheniformis DW2 is knocked out, thereby successfully obtaining Bacillus licheniformis DW2ΔmalR, from which the malR gene has been deleted. In comparison with Bacillus licheniformis DW2, the strain obtained by the construction can improve the Bacitracin yield in a fermentation broth during Bacitracin fermentation by at least 23%.

The invention provides a preparation method of bacitracin zinc. The preparation method comprises the following steps of: (1) adding a hydrochloric acid solution into bacitracin filtrate to adjust the pH to 2.5-6.0, then adding a zinc salt solution, and stirring until the solution is transparent; (2) adding a sodium hydroxide solution, adjusting the pH to 6.5-8.5, continuously stirring for 10-40 minutes, then re-measuring the pH to 6.5-8.5, and standing for more than 6 hours; and (3) carrying out suction filtration to obtain a filter cake, and drying to obtain white bacitracin zinc powder. The preparation method provided by the invention can be used for solving the defect of unstable salifying yield of bacitracin zinc in the prior art; and the zinc salt solution is added under an acidic condition, and then the pH is slowly adjusted to crystallize, so that the reaction phenomenon can be observed more easily, the reaction process can be controlled more easily, and the preparation method has relatively great industrial production and application prospects.

The invention provides a method for constructing bacillus licheniformis by knocking out an lrpC gene, a strain and an application of the strain. The bacillus licheniformis DW2 delta lrpC is obtained by knocking out the lrpC gene in a bacillus licheniformis genome with a genetic engineering method, and the yield of bacitracin of the strain in a fermentation liquid in the bacitracin fermentation process is increased by 10% or above.

The invention provides a preparation method of a bacitracin impurity L based on photocatalysis. The preparation method comprises the following steps: S1, dissolving a bacitracin raw material medicament in a solvent, and performing illuminating at 50-60 DEG C for 1-3h to obtain a crude product containing the bacitracin impurity L; and S2, separating the bacitracin impurity L from other components by adopting a preparation liquid phase, collecting a peak fraction of the bacitracin impurity L, and evaporating the collected peak fraction of the bacitracin impurity L to dryness to obtain a reference product of the bacitracin impurity L. According to the technical scheme of the invention, a photocatalytic chemical conversion method is adopted to convert bacitracin A into an epimer of the bacitracin A, namely an impurity L, under action of illumination, so that the steps are simple and convenient, efficiency is high, low-cost enrichment of an impurity crude product is realized, post-treatmentis simple, cost is low, and pollution is small.

The invention provides a sodium dodecylsulfonate-bacitracin compound and a composite oil displacement agent. The chemical structural formula of the sodium dodecylsulfonate-bacitracin compound is as follows:. The invention provides a sodium dodecylsulfonate-bacitracin compound oil displacement agent containing a sodium dodecylsulfonate-bacitracin compound obtained by reacting sodium dodecylsulfonate and bacitracin. The composite oil displacement agent can be well compounded with oil production functional bacteria to form a microbial-chemical composite oil displacement agent. The microbial-chemical composite oil displacement agent not only has the effect of microbial flooding on crude oil degradation and viscosity reduction, but also has the emulsification and viscosity reduction effect of chemical flooding, and through the biological carrying method, the chemical oil displacement agent can expand the swept volume and dispersion efficiency in the oil layer, Effectively improve the synergistic reaction efficiency of microorganisms and chemical agents to achieve the goal of 1+1>2.

The invention relates to a preparation method of bacitracin methylene disalicylate. The method comprises the following steps: extracting from a bacitracin premix, concentrating, complexing, extracting, drying and adding salt. The method is simple, and the yield is greatly improved; the biological value of the prepared bacitracin methylene disalicylate reaches 47u / mg or above, and the total yield of the bacitracin methylene disalicylate reaches 90% or above. The solvent used in the process is low in toxicity, small in dosage, easy to recycle and convenient for industrial production.

A method is used for producing a modified non-ribosomal peptide, e.g. a modified microcystin and / or modified nodularin (together CA), including the steps of: a) growing a modified non-ribosomal peptide producing cyanobacteria strain in a culture media, h) adding one or more modified substrates, preferably modified amino acids to said culture, and c) inoculating the non-ribosomal peptide, producing strain the presence of said modified substrates. The thus modified non-ribosomal peptide may be used for the therapy of various diseases.

Provided is a method of separating and purifying bacillomycin Lb from amyloliquefaciens bacilli. The method comprises the following steps of BaX030 fermentation supernate is adsorbed by macroporous adsorption resin and subjected to ethanol elution and decompress concentration, and a crude extract product is obtained; the crude extract product is subjected to chromatographic separation one time byadopting a RPC reversed phase column at -KTA avant 25, fractions are collected and merged, and decompress concentration is conducted to obtain a bacillomycin Lb crude product; the bacillomycin Lb crude product is purified for the second time by adopting the RPC reversed phase column and subjected to decompress concentration, and the target compound bacillomycin Lb is obtained. The method has the advantages that a macroporous resin adsorption method is adopted to separate and purify the bacillomycin Lb, the technology is simple and easy to implement, and the obtained lipopeptide compound bacillomycin Lb is easy to recycle and reuse, low in production cost and suitable for industrial production and has broad-spectrum anti-tumor activity.

The invention aims to provides a method for constructing bacillus licheniformis by knocking out an lysE gene, a strain and an application of the strain. The bacillus licheniformis DW2 delta lysE is constructed by knocking out the lysE gene in a bacillus licheniformis DW2 genome with a genetic engineering method, and the yield of bacitracin of the strain in a fermentation liquid in the bacitracin fermentation process is increased by 10% or above.

The invention discloses a method for producing bacitracin through fermentation. The method comprises six steps of pretreating fermentation liquor, performing resin adsorption, performing resin separation and rinsing, performing resolution and ammonia removal, performing nanofiltratoin for the first time and performing extraction and concentration. According to the prepared bacitracin, sodium thiosulfate is added, and airtight operation is performed, so that the bacitracin cannot be oxidized, the final potency of the bacitracin can be tremendously improved, and the medical value of the bacitracin is higher; and besides, the operation is simple, the preparation cycle is short, and the method can be used for massive industrial production of the bacitracin.