Bacillus licheniformis for multiplying operon bacABC copy number and knocking off recA gene and establishment method of bacillus licheniformis
A Bacillus licheniformis, copy number multiplication technology, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the difficulty of increasing the copy number of the operon bacABC, the inability to construct plasmid expression vectors, and strain maintenance Unfavorable copy number stability and other issues, to achieve the effect of increasing potency
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[0039] an operon bacABC Copy number doubling and knockout recA Bacillus licheniformis gene, said bacterial strain is Bacillus licheniformis DW2△ recA / bacABCs (Referred to as: Bacillus licheniformis DW2△ recA / bacABCs ), the strain is preserved in the China Center for Type Culture Collection in Wuhan, with the preservation number CCTCC NO: M2014251, and the preservation date is June 12, 2014. The genome of this strain also has an operon bacABC Copy number doubling and knockout recA Gene dual characteristics, strong ability to produce bacitracin.
[0040] an operon bacABC Copy number doubling and knockout recA The construction method of the bacillus licheniformis strain of gene, comprises the following steps:
[0041] (1) Plasmid T2(2)-ori (the schematic diagram of its structure, such as figure 1 shown) after restriction DNA endonuclease Xba I and Bam H I The linear T2(2)-ori plasmid was obtained by double enzyme digestion, and the linear T2(2)-ori p...
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