Zinc bacitracin

A technology of bacitracin zinc and bacitracin, applied in the direction of bacitracin, peptides, specific peptides, etc., can solve the problems of product purity and content reduction, and achieve the effect of maintaining stability, improving purity, content and potency

Active Publication Date: 2016-06-01
雅赛利(台州)制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this preparation method can also prepare bacitracin zinc, because bacitracin is extremely unstable at pH=8-11, bacitracin easily decomposes when adding inorganic zinc to form a salt at pH=8-11, resulting in product purity and content reduction

Method used

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  • Zinc bacitracin
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  • Zinc bacitracin

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0053] Preparation Example 1: Preparation of Bacitracin Fermentation Broth

[0054] Take Bacillus subtilis, directly use soybean powder, starch, corn extract, molasses and inorganic salts as the medium, the inoculation amount is 5%, and the fermentation time is 30 hours; and the temperature at the initial stage of fermentation is 30°C, after 2-3 hours the temperature Increased to 37°C, the effective number of viable bacteria reached 1.1×10 9 CFU / g, that is.

preparation example 2

[0055] Preparation Example 2: Preparation of Bacitracin Fermentation Broth

[0056] The reference application publication number is the Chinese patent preparation of CN103232959A, and its preparation process includes: (1) primary seed culture: after bacillus licheniformis is activated on the beef extract peptone slant medium for 20 hours, transfer to the shake flask in the seed medium Cultivate for 16 hours, as seed culture liquid; (2) liquid state fermentation proliferating culture: transfer the first-level seed culture liquid to the inoculum size of 7% of the fermentation medium volume in the liquid state fermentation medium, the culture temperature is 35 ℃, cultivate After 20 hours, the number of effective viable bacteria reached 5.0×10 9 CFU / g, that is.

preparation example 3

[0057] Preparation Example 3: Preparation of Bacitracin Powder

[0058] With reference to the Chinese patent with publication number CN104109191A, centrifuge the Bacillus subtilis fermentation liquid to obtain supernatant 1, then adjust the pH of the obtained supernatant 1 to 4.5-5.5 with glacial acetic acid, and leave it for 1-2 hours to obtain an acidic precipitate after the sample;

[0059] centrifuging the sample after the acidic precipitation to obtain a supernatant 2; filtering the supernatant 2 through a filter membrane to obtain a filtrate;

[0060] The filtrate is subjected to anion-exchange chromatography (column filler is CaptoQ, and the chromatographic column is BPG140 / 500) to obtain a breakthrough liquid and an equilibrium liquid; wherein the method of anion-exchange chromatography is: use an aqueous solution containing 50mM Tris, 1MNaCl and 50mM Tris Alternately treat the chromatographic column with an aqueous solution, then load the filtrate, collect the penetr...

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Abstract

The invention aims at providing high-purity and high-content zinc bacitracin. A preparation method of the zinc bacitracin comprises the following steps: S1, mixing zinc bacitracin powder and adhesive, adding water for diluting, regulating the pH value to be 5-8, granulating at the temperature of 10-25 DEG C, drying at the temperature ranging from -20 DEG C to 5 DEG C, sieving, and spraying a micro permeable membrane, so as to obtain bacitracin micro capsules; S2, regulating the pH value of an inorganic zinc aqueous solution to be 8-11, gradually adding the prepared bacitracin micro capsules, after salifying, regulating the pH value to be 5-7 with inorganic acid, stirring, and standing; and S3, separating, drying, and smashing, so that the zinc bacitracin is obtained.

Description

technical field [0001] The present invention relates to a bacitracin salt, more particularly, it relates to a bacitracin zinc. Background technique [0002] Bacitracin is an effective narrow-spectrum polypeptide antibiotic. Its antibacterial spectrum is similar to that of penicillin. It is effective against Gram-positive bacteria, some Gram-negative bacteria, spirochetes, and actinomycetes. In practical application, bacitracin is not only used as an antibiotic drug, but also an antibiotic feed additive for livestock and poultry. [0003] Bacitracin, also known as subtilisin or subtilisin, is a white powder that is easily soluble in water, ethanol, and pyridine. Studies have found that bacitracin is an unstable polypeptide composed of a variety of amino acids, including A, A1, B, C...G and other components, and it is mainly composed of bacitracin A. It is currently recognized that the components of bacitracin A, B1 and B2 have the most biological activity, which contain abo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/52A61K38/12A61K47/34A61P31/04C07K7/58C07K1/36C07K1/34C07K1/18
CPCA61K9/5031A61K38/12C07K7/58
Inventor 芦琦陈晓静
Owner 雅赛利(台州)制药有限公司
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