Method for producing bacitracin D by fermenting diatomite immobilized bacillus

A technology of bacitramycin and Bacillus subtilis, applied in the direction of microorganism-based methods, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the problems of not fixing Bacillus subtilis, etc., and achieve easy operation Effect

Pending Publication Date: 2022-07-22
NANJING UNIV OF FINANCE & ECONOMICS
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Problems solved by technology

But never used as an adsorption carrier to immobilize Bacillus subtilis for fermentation

Method used

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  • Method for producing bacitracin D by fermenting diatomite immobilized bacillus
  • Method for producing bacitracin D by fermenting diatomite immobilized bacillus
  • Method for producing bacitracin D by fermenting diatomite immobilized bacillus

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Embodiment Construction

[0027] Diatomite (Hangzhou Chongke New Materials Co., Ltd., Celite610) was sterilized at 121°C and dried for use. Pick a single colony of Bacillus subtilis fmbJ (CGMCC No.0943, see authorized patent ZL 201010576515.8 a kind of Bacillus subtilis antibacterial lipopeptide and its application in piglet feed) single colony is inoculated in nutrient broth medium (NB, Beijing Luqiao Technology Co., Ltd., specification 250g), cultured at 37°C and 180rpm for 15h, the bacterial solution was Bacillus subtilis fmbJ seed solution, and the bacterial content was 10 8 CFU / mL.

[0028] The inoculation volume ratio is 5% fmbJ seed solution (the bacterial content is 10 8 CFU / mL) in Landy fermentation medium (for detailed formula, see the document Growth inhibition of Fusarium graminearum and reduction of deoxynivalenol production in wheat grain by bacillomycin D. Journal of Stored Products Research, 2018, 75, 21-28) (50mL, 250mL conical flask), Fermentation was carried out under the condition...

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Abstract

The invention discloses a method for producing bacitracin D by fermenting diatomite immobilized bacillus, and belongs to the technical field of biology. According to the method, diatomite is added in the fermentation process of bacillus subtilis fmbJ (CGMCC No.0943) to perform immobilized fermentation on the fmbJ, so that the yield of the secondary metabolite bacitracin D of the bacillus subtilis can be remarkably increased, repeated fermentation can be performed under the condition of not adding any fresh seed solution, and the yield of the bacitracin D is remarkably increased. According to the method, the yield of bacitracin D is increased, the utilization rate of the raw materials is also increased, the production cost is reduced, and a by-product (diatomite adsorbing bacillus subtilis) obtained after fermentation can also be used for preparing a microbial preparation and can be applied to grain storage, fruit and vegetable preservation and animal feed.

Description

1. Technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for fermenting bacillus immobilized with diatomaceous earth to produce bacillimycin D. 2. Background technology [0002] With the rapid development of social economy, people pay more and more attention to green environmental protection and health. The extensive use of chemical fungicides leads to the problem of chemical residues, which will not only aggravate environmental pollution, but also seriously endanger human health. At present, the world is urgently seeking a new alternative to chemical fungicides. Bacillus subtilis (Bacillus subtilis) fmbJ is a Gram-positive bacterium belonging to the genus Bacillus, which can secrete a series of antibacterial lipopeptides such as bacteromycin D, fengycin, and surfactin. Using its metabolite antibacterial lipopeptide as a substitute for chemical fungicides, bacillomycin D has a strong inhibitory effect on a variety of...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04C07K7/58C12N11/14A23B7/154A23B9/26A23K20/195A61K38/12A61P31/10C12R1/125
CPCC12P21/02C07K7/58C12N11/14A23B7/154A23B9/26A23K20/195A61K38/12A61P31/10
Inventor 陆颖健孙静张进
Owner NANJING UNIV OF FINANCE & ECONOMICS
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