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Method for fermentation production of bacitracin

A technology of bacitracin and fermentation liquid, applied in the field of fermentation engineering, can solve the problems of reducing feedback inhibition, low fermentation level, unstable bacitracin fermentation process, etc., and achieve the effect of increasing production and ensuring stability

Active Publication Date: 2017-06-27
NCPC NEW DRUG RES & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the problem of unstable fermentation process and low fermentation level in the preparation of bacitracin by microbial fermentation in the prior art, and to provide a method by optimizing the fermentation medium formula (especially the type of nitrogen source), fermentation conditions (such as fermentation temperature), adding superparamagnetic microspheres in a specific period of time during the fermentation process to reduce feedback inhibition and increase the production of bacitracin

Method used

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  • Method for fermentation production of bacitracin
  • Method for fermentation production of bacitracin
  • Method for fermentation production of bacitracin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Preparation of Bacitracin by Fermentation in 500mL Shake Flask (Cottonseed Protein Powder, Yeast Powder 3:1)

[0034] Preparation of Seed Solution of Bacillus licheniformis Strain

[0035]The slant culture of the Bacillus licheniformis strain was inoculated on the seed medium, and cultured at 28°C, 240 rpm, for 24 h to obtain the seed liquid.

[0036] The seed medium described therein is obtained by the following method: 3.0 grams of cornstarch, 20.0 grams of glucose, 20.0 grams of yeast powder, 1.0 grams of ammonium sulfate, 2.0 grams of sodium chloride, 1.0 grams of magnesium sulfate heptahydrate, add tap water to be constant to 1000mL, pH6.5, sterilized at 121°C for 30min.

[0037] The bacitracin producing bacterium of the present invention is the strain numbered ATCC 10716.

[0038] Fermentation medium composition:

[0039] 30.0 grams of corn starch, 20.0 grams of glucose, 30.0 grams of cottonseed protein powder, 10.0 grams of yeast powder, 3.0 grams o...

Embodiment 2、5

[0042] Example 2, Preparation of Bacitracin by Fermentation in a 5L Tank (Cottonseed Protein Powder, Yeast Powder 1:1)

[0043] Preparation of Seed Solution of Bacillus licheniformis Strain

[0044] The slant culture of the Bacillus licheniformis strain was inoculated into the seed medium, and cultured at 30° C. and 200 rpm for 20 hours to obtain a seed solution.

[0045] The seed medium described therein is obtained by the following method: 5.0 grams of cornstarch, 10.0 grams of glucose, 10.0 grams of yeast powder, 2.0 grams of ammonium sulfate, 2.0 grams of sodium chloride, 1.5 grams of magnesium sulfate heptahydrate, add tap water to be constant to 1000mL, pH7.0, sterilized at 121°C for 30min.

[0046] The bacitracin producing bacterium of the present invention is the strain numbered ATCC 10716.

[0047] Fermentation medium composition:

[0048] 90.0 grams of corn starch, 60.0 grams of glucose, 45.0 grams of cottonseed protein powder, 45.0 grams of yeast powder, 6.0 gram...

Embodiment 3、10

[0051] Example 3, Preparation of Bacitracin by Fermentation in 10L Tank (Cottonseed Protein Powder, Yeast Powder 3:2)

[0052] Preparation of Seed Solution of Bacillus licheniformis Strain

[0053] The slant culture of the Bacillus licheniformis strain was inoculated on the seed medium, and cultured at 28°C, 220 rpm, for 28 h to obtain the seed solution.

[0054] The seed medium described therein is obtained by the following method: 4.0 grams of cornstarch, 15.0 grams of glucose, 15.0 grams of yeast powder, 1.0 grams of ammonium sulfate, 1.0 grams of sodium chloride, 1.0 grams of magnesium sulfate heptahydrate, add tap water to be constant to 1000mL, pH6.8, sterilized at 121°C for 30min.

[0055] The bacitracin producing bacterium of the present invention is the strain numbered ATCC 10716.

[0056] Fermentation medium composition:

[0057] 200.0 grams of corn starch, 50.0 grams of glucose, 150.0 grams of cottonseed protein powder, 100.0 grams of yeast powder, 10.0 grams of ...

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Abstract

The invention belongs to the field of fermentation engineering, and particularly relates to a method for improving yield of bacitracin by microbial fermentation. According to the method, by a step 1) of optimization of organic compounding of a nitrogen source and fermentation conditions, stability of fermentation units between batches is ensured, and the content of impurities F is reduced; and by a step 2) of adding superparamagnetic microspheres during the synthesis of bacitracin, feedback inhibition of produced bacteria by fermentation products is reduced. After fermentation, the fermentation unit of bacitracin can reach 1228U / ml, which can increase the yield of bacitracin by 123%, improve the productivity and reduce energy consumption and waste discharge.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a method for producing bacitracin by fermentation. Background technique [0002] Bacitracin is a thiazole peptide narrow-spectrum antibiotic composed of 12 amino acids, mainly produced by fermentation of Bacillus subtilis treschii and Bacillus licheniformis, effective against gram-positive cocci and bacilli, and a small number of gram-negative bacteria , Spirochetes and actinomycetes also have a certain inhibitory effect. Bacitracin has the advantages of high efficiency, low toxicity, and less residue. Therefore, bacitracin is not only an important antibiotic drug, but also a safe antibiotic feed additive for livestock and poultry. [0003] Bacitracin is a mixture of several similar polypeptide components, containing isomers with similar chemical structures such as Bacitracin A, B1, B2, B3, C1, C2, C3, E, F, etc. The main active ingredient is Bacitracin A, M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04C12R1/10
CPCC07K7/58C12N1/20C12P21/00C12P21/02
Inventor 任风芝张雪霞王耀耀耿文飞郑智慧杨海静高任龙段宝玲张炜
Owner NCPC NEW DRUG RES & DEV
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