Preparation method of bacitracin methylene disalicylate

A kind of methylene salicylic acid bacillus, methylene salicylic acid technology, applied in the direction of preparation method of peptide, bacitracin, chemical instrument and method, etc. and other problems, to achieve the effect of easy recycling, reducing degradation and short processing time

Pending Publication Date: 2022-03-25
QILU PHARMA INNER MONGOLIA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, domestic research on bacitracin methylenedisalicylate is still focused on the application, and there is almost no preparation of bacitracin methylenedisalicylate. Therefore, there is no pure bacitracin methylenedisalicylate so far. According

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  • Preparation method of bacitracin methylene disalicylate

Examples

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Comparison scheme
Effect test

Embodiment 1

[0040] A preparation method of methylenedisalicylic acid bacitracin, the steps are as follows:

[0041] Take 50kg of bacitracin premix, with bacitracin content of 20%, soak it in 8 times n-butanol for 4 hours; filter out the bacteria residue through two-in-one, collect the extract and heat it up to 65°C and concentrate it to 50L, take 4kg of methylene Dissolve salicylic acid in 4L of n-butanol, add it to the concentrated solution, and complex for 3 hours, use 15L of 0.4mol / L citric acid for three times of extraction; combine three times of acid water, adjust pH=7.6 with 1kg of caustic soda , to obtain the alkaline aqueous phase containing the target substance, and then dry it with a spray dryer to obtain 13.8kg of crude product.

[0042] Add 1.11 kg of a mixture of sodium carbonate and sodium bicarbonate to the crude product and mix evenly. The mass ratio of sodium carbonate and sodium bicarbonate is 1:8; 14.91 kg of methylenedisalicylic acid bacitracin is obtained. The bacit...

Embodiment 2

[0044] A preparation method of methylenedisalicylic acid bacitracin, the steps are as follows:

[0045] Take 50kg of bacitracin premix, with bacitracin content of 20%, soak it in 6 times n-butanol for 4 hours, filter out the bacteria residue through two-in-one, collect the extract and heat it up to 65°C to concentrate to 37.5L. Get 3.8kg methylenedisalicylic acid and dissolve it with 3.6L n-butanol, join in the concentrated solution, complex for 2 hours, use 0.4mol / L citric acid 12L, carry out three extractions; combine three acidic waters, use Adjust the pH to 7.6 with 1 kg of caustic soda to obtain an alkaline aqueous phase containing the target substance, and then dry it with a spray dryer to obtain 13.3 kg of crude product.

[0046] Add 1.06 kg of a mixture of sodium carbonate and sodium bicarbonate to the crude product and mix evenly. The mass ratio of sodium carbonate and sodium bicarbonate is 1:8; 14.36 kg of methylenedisalicylic acid bacitracin finished product is obta...

Embodiment 3

[0048] A preparation method of methylenedisalicylic acid bacitracin, the steps are as follows:

[0049] Take 50kg of bacitracin premix, with bacitracin content of 20%, soak it in 6 times ethyl acetate for 4 hours, filter out the bacteria residue through two-in-one, collect the extract and heat it to 65°C to concentrate to 37.5L. Take 3.6kg of methylenedisalicylic acid and dissolve it with 3.6L of ethyl acetate, add it to the concentrated solution, and complex for 1-3 hours. Use 12L of 0.4mol / L citric acid for three times of extraction; combine the three times of acid water, adjust the pH to 7.8 with 1kg of caustic soda to obtain an alkaline aqueous phase containing the target substance, and then dry it with a spray dryer to obtain the crude product 12.6kg.

[0050] Add 1.01 kg of a mixture of sodium carbonate and sodium bicarbonate to the crude product and mix evenly. The mass ratio of sodium carbonate and sodium bicarbonate is 1:8; 13.61 kg of methylenedisalicylic acid bacit...

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Abstract

The invention relates to a preparation method of bacitracin methylene disalicylate. The method comprises the following steps: extracting from a bacitracin premix, concentrating, complexing, extracting, drying and adding salt. The method is simple, and the yield is greatly improved; the biological value of the prepared bacitracin methylene disalicylate reaches 47u/mg or above, and the total yield of the bacitracin methylene disalicylate reaches 90% or above. The solvent used in the process is low in toxicity, small in dosage, easy to recycle and convenient for industrial production.

Description

technical field [0001] The invention relates to a preparation method of methylenedisalicylic acid bacitracin, Background technique [0002] Bacitracin methylene disalicylate (bacitracin methylene disalicylate) is the reaction product of Bacillus licheniformis secondary metabolite bacitracin and methylene salicylic acid. It is a new type of feed drug additive; it was successfully developed by Simonoff et al. in 1954 , began to be used globally in the 1980s, and the US FDA has successively approved the use of bacitracin methylene salicylate in chickens, pigs, cattle and other animals for growth promotion and prevention and control of clostridial enteritis. [0003] In 2013, bacitracin methylene salicylate obtained the approval number of pharmaceutical additives from the Ministry of Agriculture and was officially promoted in China. The approval number: Veterinary Medicine Tim Zi (2013) 130012425, and was approved for continuous use on chickens, pigs, and ducks. [0004] The Ph...

Claims

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Application Information

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IPC IPC(8): C07K7/58C07K1/14C07K1/107
CPCC07K7/58
Inventor 梁振兵杨安乐樊勇刘星初翔飞
Owner QILU PHARMA INNER MONGOLIA
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