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Fermentation medium formula for high production of bacitracin employing bacillus licheniformis and application of fermentation medium formula

A technology of Bacillus licheniformis and fermentation medium, which is applied in the field of microbial engineering, can solve the problems of bacterial aging, high pH value, and affecting fermentation yield, and achieve the effects of reducing fermentation cost, improving efficiency, and increasing fermentation yield

Inactive Publication Date: 2017-05-31
LIFECOME BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nitrogen source is an essential nutrient element that constitutes bacteria and some metabolites. Too much nitrogen will make the bacteria grow too vigorously, which will easily lead to high pH value and insufficient dissolved oxygen, which is not conducive to the accumulation of metabolites; insufficient nitrogen source , it will affect the growth and reproduction of the bacteria, and easily cause the aging and autolysis of the bacteria, thus affecting the fermentation yield.

Method used

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  • Fermentation medium formula for high production of bacitracin employing bacillus licheniformis and application of fermentation medium formula
  • Fermentation medium formula for high production of bacitracin employing bacillus licheniformis and application of fermentation medium formula
  • Fermentation medium formula for high production of bacitracin employing bacillus licheniformis and application of fermentation medium formula

Examples

Experimental program
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Effect test

Embodiment 1

[0018] The invention discloses a formula and application of a culture medium for high-yield bacitracin of Bacillus licheniformis. The culture medium includes a seed culture medium and a production fermentation medium:

[0019] The seed medium is 100g / L soybean meal or 70g / L soybean meal and 20g / L rapeseed cake; 2.5~7.5g / L cornstarch; 0.5~2.5g / L CaCO 3 ;0.1~1.0g / L (NH 4 ) 2 SO 4 .

[0020] The production fermentation medium is 100g / L soybean meal or 70g / L soybean meal and 20g / L rapeseed cake, 15-25g / L cornstarch, 4-8g / L CaCO 3 and 0.5~2g / L (NH 4 ) 2 SO 4 .

[0021] Bacillus licheniformis culture bacitracin steps are as follows:

[0022] Seed culture: first activate Bacillus licheniformis, that is, inoculate the PA bottle with 5mL LB medium from the glycerol tube at 1% by volume, 180-300rpm, temperature 37°C, cultivate for 10-14 hours, and then inoculate the strain The activated bacterial solution is inoculated with the seed medium at a volume percentage of 1% inoculum,...

Embodiment 2

[0025] Experimental purpose and method: In order to verify the influence of different seed culture media and production fermentation media in the present invention on the bacitracin fermentation yield, this example uses the media formula and implementation method described in Example 1 for experimental verification. The specific experimental groups are as follows:

[0026] Table 1 Effects of different seed media and production fermentation media on the titer of bacitracin

[0027]

[0028] Experimental results:

[0029] Through the combination of the above-mentioned different seed media and production fermentation media, it can be found that under the same cultivation conditions, using rapeseed cake instead of part of the soybean meal to modify the media can effectively increase the titer of bacitracin.

Embodiment 3

[0031] Experiment purpose and method: In order to study the influence of a single nitrogen source on the fermentation of Bacillus licheniformis, in this example, 100 g / L of soybean meal and peanut cake powder were added to the fermentation medium on the basis of keeping the other components unchanged except for the nitrogen source. , soybean cake powder, low-protein rapeseed cake, high-protein rapeseed cake, cottonseed cake, sesame cake, fish meal as a single nitrogen source, and compare their effects on the fermentation of bacitracin. The low-protein rapeseed cake and high-protein rapeseed cake were purchased from the Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, and the soybean meal, peanut cake powder, soybean cake powder, cottonseed cake, sesame cake and fish meal were purchased from the state-owned Sanhu Farm Feed Factory in Hubei Province. Considering that different types of nitrogen sources may have different fermentation periods, the prod...

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Abstract

The invention relates to the field of microbial engineering and discloses a fermentation medium formula for high production of bacitracin employing bacillus licheniformis and application of the fermentation medium formula. One part of bean pulp is replaced with cheap rapeseed dregs rich in cysteine, so that the fermentation yield of the bacitracin is significantly improved, and the valence of the bacitracin reaches 849.3U / mL and is improved by 12.4% in comparison with that of an original culture medium. Meanwhile, the market price of the bean pulp and the rapeseed dregs is investigated, the price of the bean pulp fluctuates within a small range of about 3200yuan / t, the mean price of the rapeseed dregs fluctuates within a small range of about 2800yuan / t, the fermentation cost is significantly reduced by replacement of fermentation raw materials, and the benefits of the bacitracin fermented by the bacillus licheniformis is improved.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to a formulation of a fermentation medium for high-yield bacitracin of bacillus licheniformis and its application. Background technique [0002] Bacitracin is a polypeptide antibiotic composed of 10 amino acids and 12 amino acid residues. It is light yellow or off-white powder, odorless and bitter. Bacitracin contains various components such as A, B1, B2, B3, D1, D2, D3 and E, among which component A has the highest activity and the most abundant content. Bacitracin can effectively inhibit Gram-positive pathogenic bacteria and some Gram-negative bacteria. At the same time, bacitracin has the advantages of almost no absorption in the intestine, rapid excretion, and no residue in the body, so it is widely added to feed. [0003] At present, bacitracin is produced by microbial fermentation, and its main production strains are Bacillus licheniformis and Bacillus subtilis. The com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04C12R1/10
CPCC07K7/58C12P21/02
Inventor 陈守文蔡冬波郑惠李阳李俊辉陈晓斌楼丽君万剑桥
Owner LIFECOME BIOCHEM
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