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Method of preparing ethanol through cellulose solid phase enzymolysis and liquid fermentation coupling and its installation

A liquid fermentation and cellulose technology, which is applied in biochemical equipment and methods, enzymology/microbiology devices, bioreactor/fermenter combinations, etc. Problems such as large water content, to achieve the effect of reducing feedback inhibition and avoiding inhibition

Inactive Publication Date: 2004-05-05
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present invention is to overcome the disadvantages of low product sugar concentration, low ethanol concentration, large amount of cellulase in raw materials, and large water content of fermentation residues in the traditional device for synchronous saccharification and fermentation of cellulose liquid to prepare ethanol. Provides a device for preparing ethanol by coupling solid-phase enzymatic hydrolysis of cellulose-liquid fermentation
[0006] Another object of the present invention is to provide a method for preparing ethanol by coupling solid-phase enzymatic hydrolysis-liquid fermentation of cellulose, which overcomes the large amount of cellulase in raw materials in the prior art, the solid phase state is not conducive to rapid ethanol fermentation, The saccharification and fermentation temperature is difficult to adjust, the water content of the produced ethyl alcohol is large, and the purification of ethanol consumes a lot of energy, etc.

Method used

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  • Method of preparing ethanol through cellulose solid phase enzymolysis and liquid fermentation coupling and its installation
  • Method of preparing ethanol through cellulose solid phase enzymolysis and liquid fermentation coupling and its installation

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The height-to-diameter ratio of the cellulose solid-phase enzymolysis-liquid fermentation reactor 2 of the present embodiment is 12, two ethanol adsorption columns 10 are used in parallel, the volume ratio of the outer cylinder 20 and the inner cylinder 4 is 1:5, and the outer cylinder 20 The height-to-diameter ratio of the inner cylinder 4 is 10, the height-to-diameter ratio of the inner cylinder 4 is 50, and the height-to-diameter ratio of the upper head 11 is 0.5;

[0036] Its preparation ethanol step is as follows:

[0037] 1. Add sterilized cellulose raw material and cellulase into cavity 3, keep at 40°C for 40 hours to generate cellulose hydrolyzate;

[0038] 2. From the top of the cavity between the inner and outer cylinders, rinse the cellulose enzymatic hydrolyzate in the cavity with sterile water, and the obtained enzymatic liquid flows into the lower head 7 located at the lower end of the main body of the reactor, and circulates with an enzymatic liquid The ...

Embodiment 2

[0047] Using the device for preparing ethanol by coupling solid-phase enzymatic hydrolysis of cellulose-liquid fermentation in Example 1, the solid-phase enzymatic hydrolysis of cellulose, liquid fermentation and ethanol recovery are carried out according to the following steps, and the specific steps are as follows:

[0048] a. Put the wheat straw raw material treated by non-polluting steam explosion into the cavity 3 between the inner and outer cylinders of the solid-phase enzymatic hydrolysis-liquid fermentation reactor 2, pass through 0.1Mpa high-pressure steam to sterilize for 30min, and cool to 50°C , add 10IU filter paper enzyme activity / g pretreated cellulose raw material according to aseptic operation, and incubate at 40°C for 30hr;

[0049] b. Rinse the cellulose hydrolyzate in the cavity 3 between the inner and outer cylinders with sterile water, store the obtained enzymolyzate in the lower head 7, and pump the enzymolyzate into the inner cylinder 4 with the enzymoly...

Embodiment 3

[0055] Make a cellulose solid-phase enzymatic hydrolysis-liquid fermentation coupled device for preparing ethanol. The height-to-diameter ratio of the cellulose solid-phase enzymatic hydrolysis-liquid fermentation reactor 2 in this embodiment is 15, and four ethanol adsorption columns 10 are used in parallel. The volume ratio of the cylinder 20 and the inner cylinder 4 is 1:8, the height-diameter ratio of the outer cylinder 20 is 12, the height-diameter ratio of the inner cylinder 4 is 55, and the height-diameter ratio of the upper head 11 is 0.2;

[0056] Add cellulose raw materials and cellulase to the cavity 3 between the inner and outer cylinders (the solid-phase enzymatic hydrolysis area of ​​cellulose) to carry out solid-phase enzymatic hydrolysis of cellulose, and the enzymatic hydrolyzate produced is rinsed and filtered by the lower filter plate 5 before entering the The lower head 7 is stored; in the inner cylinder 4, the enzymolysis solution pumped in from the lower h...

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Abstract

A process and equipment for preparing alcohol by the coupled solid-phase cellulose enzymolysis and liquid fermentation are disclosed. Its equipment is composed of vertical reactor consisting of internal and external cylinder, upper and lower closure heads, upper and lower plate filters, air-floating valve connected to lower part of internal cylinder, CO2 gas circulating pump, and enzymolyzed liquid circulating pump. Said process includes adding cellulose and cellulose into the cavity between internal and external cylinders, leaching the enzymolyzed substance of cellulose, pumping the liquid into internal cylinder, adding yeast into internal cylinder, fermenting, and separating and recovering alcohol.

Description

technical field [0001] The invention belongs to the technical field of cellulose fermentation industry, and in particular relates to a method and a device for preparing ethanol by coupling solid-phase enzymatic hydrolysis of cellulose and liquid fermentation. Background technique [0002] Biomass generally refers to all plants that receive light and heat from the sun and store part of the energy through photosynthesis. Converting the energy stored by plants in the form of carbohydrates into electrical energy per year can reach 500,000 kWh per capita, which is 420 times the current annual per capita energy consumption in the world. Biomass as the research object of development and utilization generally refers to agricultural and forestry wastes, such as straw, bagasse, rice bran, wood chips, etc. Biomass is attractive for its renewable, huge output, storable, carbon cycle and other advantages, and among a variety of clean follow-up energy sources, it is the only one that can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12P7/06
CPCY02E50/17C12M21/12C12M23/56Y02E50/10
Inventor 陈洪章李佐虎
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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