Method for producing ethanol by carrying out high temperature enzymolysis and fermentation on lignocellulose

A technology of lignocellulose and high-temperature enzymatic hydrolysis, which is applied in the field of ethanol production by high-temperature enzymatic hydrolysis and fermentation of lignocellulose, can solve problems far from reaching the level of industrialization, improve the ability to withstand high temperatures, reduce usage, and reduce feedback inhibitory effect

Inactive Publication Date: 2016-06-15
LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY
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Problems solved by technology

[0006] The optimal enzymatic hydrolysis temperature of the cellulase used in the traditional cellulase process is 45-50°C. Studies have shown that enzymatic hydrolysis at a higher temperature can improve the enzymatic hydrolysis efficiency and reduce the use of cellulase. The existing high temperature resistant fiber Sulfase can be used for high-temperature enzymatic fermentation to produce ethanol, but it is immature and far from industrialized

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  • Method for producing ethanol by carrying out high temperature enzymolysis and fermentation on lignocellulose

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Embodiment 1

[0028] The lignocellulosic raw material used in the embodiment of the present invention is dry corn stalk, wherein 38.2wt% of cellulose, 22.1wt% of hemicellulose, 20.2wt% of lignin, and 3.9wt% of ash are pulverized with a pulverizer until the particle size is 1- 5mm. Dilute acid steam explosion was used for pretreatment, the reaction temperature was 190°C, the reaction time was 5min, the solid-liquid ratio was 1:2, and the concentration of dilute sulfuric acid was 2.0wt%. After coming out from the steam explosion device, adjust the pH with NaOH to be 5.0, wherein the dry matter concentration is 32wt%, the cellulose content in the dry matter is 40wt%, and the pretreatment dry matter recovery rate is 96wt%.

[0029] Yeast seed liquid culture medium is 2wt% glucose, 2wt% peptone and 1wt% yeast extract, sterilized at 115°C for 30min for later use. The preparation of yeast seed liquid is divided into 3 stages of cultivation: the first stage uses the inoculation loop to scrape 1-2 ...

Embodiment 2

[0039] The method for producing ethanol by high-temperature enzymatic hydrolysis and fermentation of lignocellulose of the present invention comprises the following contents:

[0040] (1) Combined pretreatment of lignocellulosic raw material corn straw with dilute acid steam explosion to obtain pretreated raw materials;

[0041] (2) Add the pretreated raw materials, cellulase, water and xanthan gum into the enzymolysis tank for pre-enzymolysis, control the dry matter concentration of the enzymolysis system to 15wt%, and control the pre-enzymolysis temperature at 54°C; The residence time of the enzymolysis feed liquid is 8h; the pH of the pre-enzymolysis is 4.5; the addition of xanthan gum is 0.08g / L; The ratio is 5IU / g cellulose;

[0042] (3) After the pre-enzymolysis, the feed liquid is poured into the fermenter, and the temperature-resistant Saccharomyces cerevisiae is added for simultaneous saccharification and fermentation. Synchronous saccharification and fermentation re...

Embodiment 3

[0045] The method for producing ethanol by high-temperature enzymatic hydrolysis and fermentation of lignocellulose of the present invention comprises the following contents:

[0046] (1) Combined pretreatment of lignocellulosic raw material corn straw with dilute acid steam explosion to obtain pretreated raw materials;

[0047] (2) Add pretreated raw materials, cellulase, water and xanthan gum into the enzymolysis tank for preenzymolysis, control the dry matter concentration of the enzymolysis system to 25wt%; control the residence time of the enzymolysis feed solution to 48h The pH of the pre-enzymolysis is 5.5, and the temperature is 62°C; the addition of xanthan gum is 0.24g / L; the addition of the cellulase makes the ratio of the cellulase to the cellulose in the pretreated raw material 25IU / L g cellulose;

[0048] (3) After the pre-enzymolysis, the feed liquid is poured into the fermenter, and the temperature-resistant Saccharomyces cerevisiae is added for simultaneous s...

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Abstract

The invention discloses a method for producing ethanol by carrying out high temperature enzymolysis and fermentation on lignocellulose. The method for producing the ethanol by carrying out the high temperature enzymolysis and fermentation on the lignocellulose comprises the following steps: (1) pretreating a lignocellulose raw material, so as to obtain a pretreated raw material; (2) adding the pretreated raw material, cellulase, water and xanthan gum into an enzymatic vessel for carrying out pre-enzymolysis, and controlling dry material concentration of an enzymolysis system to be 15-25wt%; (3) pumping an enzymolyzed feed liquid into a fermentation tank, adding temperature-resistant saccharomyces cerevisiae, and carrying out simultaneous saccharification and fermentation; and (4) pumping fermentation liquor into a rectification device and separating ethanol. The method disclosed by the invention has the advantages that the xanthan gum is added into the enzymolysis system, high temperature tolerance of cellulase is improved, activity of the cellulase under high temperature enzymolysis condition is high, usage amount of the cellulase is reduced, enzymolysis efficiency of the lignocellulose is improved, and economy is improved.

Description

technical field [0001] The invention belongs to the field of biomass energy, and in particular relates to a method for producing ethanol by high-temperature enzymatic hydrolysis and fermentation of lignocellulose. Background technique [0002] As the world's population grows and countries become more industrialized, energy consumption continues to rise. Oil is the main resource to meet energy demand, but oil resources are limited. Scientists predict that crude oil production will drop from 25.8 billion barrels (3.525 billion tons) in 2009 to 5 billion barrels by 2050. As a renewable transportation fuel, bioenergy can effectively reduce the room temperature effect, slow down environmental pollution, and at the same time change the existing unequal oil supply and demand relationship and maintain a continuous supply, which has obvious advantages over mineral fuels. Compared with other bio-energy sources such as biodiesel, the production of fuel ethanol has already reached a co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCC12P7/10C12P19/14C12P2203/00Y02E50/10
Inventor 姚秀清李炎炎陆光
Owner LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY
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