New application of chromaffin cell of adrenal medulla or opium peptide energy cell

A technology of chromaffin cells and adrenal glands, which is applied in the field of adrenal medulla chromaffin cells or opioid peptidergic cells, can solve the problems of APA microencapsulated bovine adrenal medulla chromaffin cell withdrawal syndrome and other problems that have not yet been found, and achieve good immunity. Protective effect, reduction of negative feedback inhibition, and effect of reducing withdrawal symptoms

Active Publication Date: 2010-02-24
薛毅珑
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there have been no reports on the use of APA microencapsulated bovine adrenal medulla chromaffin cells for the treatment and / or alleviation of withdrawal syndrome at home and abroad.

Method used

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  • New application of chromaffin cell of adrenal medulla or opium peptide energy cell
  • New application of chromaffin cell of adrenal medulla or opium peptide energy cell
  • New application of chromaffin cell of adrenal medulla or opium peptide energy cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: Isolation and purification of BCC

[0042] 1. Get 12 fresh bovine adrenal glands from the slaughterhouse (the ischemia time at room temperature is no more than 1 hour), and transport them back to the laboratory quickly under refrigerated conditions for future use.

[0043] 2. Inject collagenase I (collagenase I) solution with a concentration of 1g / L from the vein of the adrenal glands, inject 5ml of collagenase I solution into each adrenal gland, and then place it at 37°C for 30 minutes to allow the collagenase to interact with bovine adrenal cells The colloids are fully responsive.

[0044] 3. Cut the cortex along the longitudinal axis of the adrenal gland, separate the medulla and cut it into pieces.

[0045] 4. Add 60ml of collagenase I solution with a concentration of 1g / L to all the chopped medullary tissue, and let it stand for another 30 minutes.

[0046] 5. Filter with a steel mesh with a mesh of 170 mesh (88 μm) to collect the liquid under the m...

Embodiment 2

[0052] Embodiment 2: Preparation of microencapsulated animal cell drug

[0053] 1. Use the BCC obtained by the method of Example 1, and centrifuge to obtain the precipitate of BCC, wash with physiological saline and dilute to 1 ml, and move it into a centrifuge tube.

[0054] 2. Count with the disc blue staining method, and the total number of cells obtained is 3X10 6 cells, in which the purity of BCC was 82%.

[0055] 3. Add 1ml of sodium alginate solution with a concentration of 15g / L to it, and make it into a suspension by stirring.

[0056] 4. Spray the suspension into 100ml of calcium chloride solution with a concentration of 100mmol / L using an electrostatic droplet generator (electrostatic droplet generator, manufactured by the University of Toronto, Canada), and obtain a diameter of 180-500 μm after 10 minutes. Calcium alginate beads containing cells within the range were precipitated, and the supernatant was removed after the precipitation was complete.

[0057] 5. ...

experiment example 1

[0061] Experimental example 1: Effect of microencapsulated BCC on withdrawal syndrome in rats

[0062] Experimental Materials

[0063] 1 Experimental drugs: morphine hydrochloride, methadone, naloxone hydrochloride, normal saline, NO kit, SOD kit, prostaglandin E 2 Radioimmunization Kit, Heparin Sodium Injection, Indomethacin Injection, Chloral Hydrate Injection.

[0064] 2 Microencapsulated chromaffin cells.

[0065] 3 Experimental animals: Wistar rats, about 250g.

[0066] experimental method

[0067] 1 Establishment of rat morphine-dependent model (referring to the method of Ling GSF et al.): A total of 150 Wistar rats weighing about 250 g, male and female, were randomly divided into 5 groups, with 30 rats in each group. Groups 1-4 are morphine-dependent model groups, subcutaneously inject morphine twice a day (8:00 am and 6:00 pm), the dose starts from 20 mg / kg body weight per day, increases 20 mg / kg body weight every day, and administers for 5 days. The final dose is...

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Abstract

The present invention relates to a new application of chromaffin cell of adrenal medulla or opium peptide energy cell, particularly an application of curing and / or releasing withdrawal syndrome causedby addiction to drugs or drug abuse.

Description

field of invention [0001] The present invention relates to a new application of adrenal medulla chromaffin cells or opioid peptidergic cells, especially for treating and / or alleviating withdrawal syndrome caused by drug addiction or drug abuse. Background technique [0002] Drug dependence and addiction caused by drug use and drug abuse have become increasingly serious social problems. Commonly used treatment methods include: substitution and tapering therapy (such as methadone, dihydroedorphine, etc.), subhibernation therapy, traditional Chinese medicine therapy, etc., but there is no generally accepted ideal method so far. [0003] At present, it is believed that the addiction mechanism of opioids is related to the function of opioid receptors. Under normal physiological conditions, opioid receptors are affected by a certain basal level of endogenous opioid polypeptide (EOP), when exogenous opioid compounds (EOC) such as morphine are given, morphine occupies the remaining...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P25/36
Inventor 薛毅珑刘光华
Owner 薛毅珑
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