The present invention discloses an adipose mesenchymal stem cell preparation kit, the kit includes a fat storage solution, a fat washing liquid, a fat decomposition liquid, a cell washing liquid, a cell culture liquid, a cell digestion liquid and a cell cryopreservation liquid; the fat storage solution is a DMEM, DMEM / F12, MEM or RPMI-1640 medium including 100-200ug / ml of penicillin, 100-200ug / ml of streptomycin and 2.5-5 mug / ml of amphotericin; the fat washing liquid is normal saline, PBS, D-Hanks or HBS including 100-200ug / ml of penicillin, 100-200ug / ml of streptomycin and 2.5-5mug / ml of amphotericin; the fat decomposition liquid is a DMEM, DMEM / F12, MEM or RPMI-1640 medium including 0.75-2mg / ml of collagenase I; the cell washing liquid is PBS, D-Hanks or HBSS; the cell culture liquid is a mesenchymal stem cell serum-free growth medium, the cell digestion liquid is a PBS, D-Hanks or HBSS solution including 1.25-2.5mg / ml of Trypsin-EDTA; and the cell cryopreservation liquid is a mesenchymal stem cell serum-free growth medium including 60-200mg / ml of DMSO.