Mycoplasma pneumoniae recombinant antigen and application thereof

Abstract

The invention relates to a mycoplasma pneumoniae recombinant antigen and application thereof. The mycoplasma pneumoniae recombinant antigen comprises a PIA fragment, a first binding peptide, a PIB fragment, a second binding peptide and a P30 fragment. Experiment results show that the recombinant antigen can obtain different types of antigen dominant epitopes after being purified in different ways,and the various types of antigen dominant epitopes are stable in expression, namely the different types of antigen dominant epitopes can be stably expressed as required. The matching and screening works of the antigen can be greatly reduced during detection; the detection rate is high, and the specificity is high during the detection of mycoplasma pneumoniae.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a mycoplasma pneumoniae recombinant antigen and its application. Background technique

[0002] Mycoplasma pneumoniae (Mycoplasma Pneumoniac, Mp) is a pathogenic mycoplasma, and the primary atypical pneumonia caused by it accounts for about 1 / 2 of non-bacterial pneumonia, and the incidence of Mp infection is on the rise in recent years. In addition to primary atypical pneumonia, Mp can still cause other respiratory infectious diseases such as bronchitis, pharyngitis, etc., as well as complications in the nervous system, blood system, cardiovascular system, skin, muscle, and joints. About 30% of pneumonia in the general population is caused by Mycoplasma pneumoniae. The respiratory damage and various extrapulmonary complications caused by Mp infection have attracted widespread attention.

[0003] The diagnosis of Mycoplasma pneumoniae mainly relies on isolation and culture and serolog...

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