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Vaccine composition for resisting pig mycoplasma pneumonia and infectious pleuropneumonia and preparation method

A technology of mycoplasma pneumonia and vaccine composition, which is applied in the direction of bacterial antigen components, antibody medical components, antibacterial drugs, etc., can solve the problem that there is no dual combination vaccine that can prevent and treat mycoplasma pneumonia and porcine infectious pleuropneumonia at the same time, and achieve Immunization is cheap, labor-intensive, and practical

Active Publication Date: 2014-03-12
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, there is no dual combination vaccine capable of simultaneously preventing and treating mycoplasma pneumonia and porcine infectious pleuropneumonia

Method used

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  • Vaccine composition for resisting pig mycoplasma pneumonia and infectious pleuropneumonia and preparation method
  • Vaccine composition for resisting pig mycoplasma pneumonia and infectious pleuropneumonia and preparation method
  • Vaccine composition for resisting pig mycoplasma pneumonia and infectious pleuropneumonia and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Isolation and identification of Mycoplasma hyopneumoniae HN0613

[0048] 1. Materials and methods

[0049] (1) Material

[0050] ① source of disease material

[0051] In 2006, 16 suspected lungs with mycoplasma swine pneumonia were collected from various places in Henan Province.

[0052] ② Culture medium

[0053] Mycoplasma hyopneumoniae Friis medium was purchased from BD Company; pig serum was purchased from GIBCO Company; phenol red indicator was purchased from American AMRESCO Company.

[0054] ③ Reagent

[0055] Biochemical reagents and chemical reagents were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0056] Bacterial Genomic DNA Extraction Kit was purchased from TIANGEN Company, and Dieter's Staining Kit was purchased from Chongqing Pangtong Medical Instrument Co., Ltd. Biochemical identification reagents were purchased from Hangzhou Tianhe Microbiological Reagent Co., Ltd.

[0057] ④ Negative and positive serum

[0058] Rabbit anti-M...

Embodiment 2

[0094] Embodiment 2: Identification of the isolation of serum type 1 LC strain, type 5 YC strain, and type 7 YS strain

[0095] 1. Materials and Methods

[0096] (1) Disease materials to be tested

[0097] The disease materials of pigs with suspected pleuropneumonia were collected from 10 pig farms in Henan, Hubei and other provinces and cities.

[0098] (2) PCR primer sequence

[0099] PF-5'CCGACTTTTAAATCCGT3', PR-5'GAACAGTTGTTCGCTAA3'

[0100] (3) mice

[0101] Bought from Animal Experiment Center of Henan Province.

[0102] (4) Isolation and purification of pathogenic bacteria and liquid culture

[0103] Aseptically take dying pig lungs, throat tonsils and other tissues, inoculate them on TSA (containing 1wt% NAD) agar medium, place in 10% CO 2 After culturing at 37°C for 24-36 hours, select a typical single colony for purification and culture. The purified single colonies were selected and inoculated in TSB (containing 1 wt% NAD) liquid culture medium, and cultured ...

Embodiment 3

[0129] Example 3: Preparation of Mycoplasma hyopneumoniae antigen and Actinobacillus pleuropneumoniae antigen

[0130] 1. The source of bacteria (virus) strains

[0131] The selected Actinobacillus pleuropneumoniae strains are: the preservation number of the serum type 1 LC strain is CCTCC M2011458; the preservation number of the serum type 5 YC strain is CCTCC M2011459; the preservation number of the serum type 7 YS strain is CCTCC M2011460. The date of deposit is December 9, 2011.

[0132] The selected Mycoplasma hyopneumoniae is HN0613, and the preservation number is CCTCC No. M2012230. The date of deposit is June 13, 2012.

[0133] 2. Preparation and inspection of vaccine semi-finished products

[0134] (1) Preparation of seeds for production

[0135] ① Actinobacillus pleuropneumoniae:

[0136] Propagation of primary seeds

[0137] Streak and inoculate the LC, YC, and YS strains of freeze-dried strains on TSA (soybean casein agar) plates respectively, and inoculate t...

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Abstract

The invention relates to a vaccine composition for resisting pig mycoplasma pneumonia and infectious pleuropneumonia and a preparation method. The vaccine composition comprises pig mycoplasma hyopneumoniae antigens and pig actinobacillus pleuropneumoniae antigens, is simple in immunization procedure, not only is capable of generating antibodies, but also has the functions of toxicity attack protection and effectively controlling pig mycoplasma pneumonia and infectious pleuropneumonia. The vaccine composition has the immunization effect same to an independently injected vaccine, is small in side reaction, long in immunization period, less in consumed time, less in consumed labor, small in encroaching on a pig. The vaccine composition is simple in production technology, low in immunization cost and strong in practicality.

Description

technical field [0001] The invention belongs to the technical field of livestock biopharmaceuticals, and relates to a dual multivalent vaccine composition against swine mycoplasma pneumonia and infectious pleuropneumonia and a preparation method thereof. Background technique [0002] Swine asthma, also known as Mycoplasma pneumoniae of swine (MPS) or Swine Enzootic Pneumonia (SEP), is a contact chronic respiratory infectious disease caused by Mycoplasma hyopneumoniae , are ubiquitous throughout the world. The diseased pigs mainly showed cough and wheezing, growth retardation, low feed conversion rate, and normal body temperature. During anatomy, the main lesions were in the lungs, especially in the heart lobe, middle lobe, and apical lobe of the two lungs, which were characterized by pancreatoid changes and fleshy changes. High morbidity and low mortality. Studies in recent years have found that Mycoplasma hyopneumoniae is mixed with porcine reproductive and respiratory s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A61K39/295A61K39/102A61P31/04A61P31/14A61P31/22A61P31/20A61P31/16
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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