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Porcine circovirus type 2, porcine reproductive and respiratory syndrome bivalent vaccine and preparation method thereof

A technology for porcine circovirus and respiratory syndrome, applied in antiviral agents, viral antigen components, pharmaceutical formulations, etc., can solve the problems of immune antigen component interference, time-consuming and labor-intensive, and inconvenient to use.

Active Publication Date: 2014-09-24
JIANGSU NANNONG HI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many types of vaccines for pigs. Multiple vaccines and multiple injections of immunization are time-consuming and labor-intensive. On the other hand, they will increase the stress response of pigs and affect the immune effect.
[0003] At present, there are single vaccines corresponding to porcine reproductive and respiratory syndrome and porcine circovirus disease on the market, which need multiple separate injections. The stress response of the vaccinated animals is strong and inconvenient to use. Although the multi-vaccine is more convenient to use, However, there is often interference between the immune antigen components of the vaccine

Method used

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  • Porcine circovirus type 2, porcine reproductive and respiratory syndrome bivalent vaccine and preparation method thereof
  • Porcine circovirus type 2, porcine reproductive and respiratory syndrome bivalent vaccine and preparation method thereof
  • Porcine circovirus type 2, porcine reproductive and respiratory syndrome bivalent vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] ——Preparation of porcine circovirus type 2 and porcine reproductive and respiratory syndrome dual vaccine

[0075] Taking porcine circovirus type 2 and porcine reproductive and respiratory syndrome dual vaccine batches 13001, 13002, and 13003 batches as examples

[0076] 1. Preparation and testing of porcine circovirus type 2 antigen solution

[0077] (1) PCV2SH strain (CGMCC No.2389, provided by Jiang Ping, Nanjing Agricultural University) kind of virus is inoculated to the PK15-B1 cell (provided by Jiang Ping, Nanjing Agricultural University) by (V / V) 3% to grow into a good monolayer 4% calf serum DMEM cell maintenance solution (V / V) was added to the bottle provided by the professor, and cultured at 37°C. Harvest after 4 days of culture, freeze and thaw three times at -20°C, harvest the cell culture, and take a small amount of samples for sterility testing and determination of virus content. After harvesting, the virus liquid was stored at -20°C.

[0078] (2) Steri...

Embodiment 2

[0101] ——Examination of porcine circovirus type 2 and porcine reproductive and respiratory syndrome dual vaccine

[0102] Taking porcine circovirus type 2 and porcine reproductive and respiratory syndrome dual vaccine batches 13001, 13002, and 13003 batches as examples

[0103] 1. Trait test

[0104] The properties of the three batches of dual vaccine prepared by the above method were tested, and the vaccine was a light yellow spongy loose mass, which was easy to separate from the bottle wall and dissolved quickly after adding the vaccine diluent.

[0105] 2. Sterility test, mycoplasma test

[0106] The three batches of vaccines were tested according to the method in the appendix of "Chinese Veterinary Pharmacopoeia", and the three batches of vaccines were all sterile and free of mycoplasma growth.

[0107] 3. Safety inspection

[0108] Dilute three batches of dual vaccine with vaccine diluent to contain 10 piglets per milliliter, intramuscularly inject 3 healthy piglets wi...

Embodiment 3

[0131] - Sterility test of vaccine diluent

[0132] Get 3 batches of vaccine diluents and carry out sterility test according to the appendix of "Chinese Veterinary Pharmacopoeia", and the test result shows aseptic growth in the vaccine diluents.

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Abstract

The invention relates to a porcine circovirus type 2, porcine reproductive and respiratory syndrome bivalent vaccine and a preparation method thereof. The bivalent vaccine is a lyophilized vaccine, and comprises a porcine circovirus type 2 SH strain inactivated virus and a respiratory syndrome virus R98 strain live virus, while an adjuvant is added into the vaccine diluent, so that the immunizing effect of the vaccine can be further improved. Immune interference does not exist among the bivalent lyophilized vaccine antigens, and the quality inspection of the vaccine finished product can achieve the quality standard of a single vaccine, and the immune protection effect of the vaccine finished product is better than that of a single vaccine; the bivalent vaccine can be used for simplifying the immune procedure, reducing immune cost and reducing the stress reaction of an immunized pig, and thus the bivalent vaccine has great market application values.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and relates to a dual vaccine for porcine reproductive and respiratory syndrome and porcine circovirus disease and a preparation method thereof. Background technique [0002] Porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS), also known as blue ear disease, is an acute, highly severe disease of pigs caused by porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus, PRRSV). Contact infectious diseases are the main infectious diseases in large-scale pig farms in my country. Porcine circovirus type 2 (PCV2) can cause immunosuppression in pigs, leading to a decrease in the body's resistance, thereby inducing mixed infections and secondary infections of various viruses and / or bacteria. These two diseases are very harmful to the pig industry and have caused huge economic losses to t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K39/39A61P31/20A61P31/14
Inventor 缪芬芳何叶峰孙石静何海蓉董彦鹏刘怡胡静雅
Owner JIANGSU NANNONG HI TECH
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