Trivalent inactivated vaccine of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and porcine pseudorabies virus and preparation method thereof
A technology for respiratory syndrome and porcine circovirus, applied in antiviral agents, viral antigen components, pharmaceutical formulations, etc., can solve problems such as shortages, improve safety, improve safety and immune efficacy, and reduce epidemic prevention costs Effect
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Embodiment 1
[0031] Embodiment 1: Preparation and inspection of porcine reproductive and respiratory syndrome-porcine circovirus disease type 2-porcine pseudorabies triple inactivated vaccine
[0032] 1 Materials and methods:
[0033] 1.1 Source of virus strain:
[0034] Selected strain is porcine reproductive and respiratory syndrome virus NVDC-JXA1 strain (referring to Chinese patent CN100439494C), porcine circovirus type 2 is SH strain (referring to Chinese patent CN101240264A), pseudorabies strain is Fa strain (purchased from Chinese Veterinary Medicine Drug Control Office),
[0035] 1.2 Preparation and inspection of virus liquid for seedling production
[0036] (1) Preparation and inspection of PRRSV virus liquid
[0037] Inoculate Marc-145 into a culture solution with a pH value of 7.0-7.4 and a polyester fiber carrier tank, mix Marc-145 cells with polyester fiber evenly, start the attachment procedure for 4 hours, and make Marc-145 cells adhere to the on polyester fiber. Switch...
Embodiment 2
[0113] Embodiment 2, porcine reproductive and respiratory syndrome-porcine circovirus disease type 2-porcine pseudorabies triple inactivated vaccine and single vaccine (porcine reproductive and respiratory syndrome inactivated vaccine or porcine circovirus type 2 inactivated vaccine or Comparison of immune effects of porcine pseudorabies inactivated vaccine).
[0114] 1. Materials
[0115] Porcine reproductive and respiratory syndrome-porcine circovirus disease type 2-porcine pseudorabies triple inactivated vaccine, the laboratory product in Example 1, batch number: 1101; porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain ), source: Luoyang Pulaike Bioengineering Co., Ltd., batch number: 110213; porcine circovirus type 2 inactivated vaccine (SH strain), source: Luoyang Pulaike Biological Engineering Co., Ltd., batch number: 110115; porcine pseudorabies Live vaccine (Ea strain), source: Wuhan Keqian Biological Products Co., Ltd., batch number: ...
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