Preparation method of zeranol complete antigen

A technology of zearalanol and complete antigen, applied in chemical instruments and methods, animal/human protein, serum albumin, etc., can solve the problems of ZER antibody preparation and detection reports, low detection sensitivity, etc., and achieve strong practicability , High antigen titer, simple operation

Inactive Publication Date: 2017-05-31
SHAANXI YUHANG ELECTRONICS
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few reports on ZER antibody preparation and detection, and the detection sensitivity is not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method of zearalanol complete antigen, comprising the following steps:

[0020] (1) Weigh 2 mg of ZER and dissolve it in 3 mL of acetone, add excess potassium carbonate 10 mg and 1 microliter of ethyl 4-bromobutyrate, at 60 ° C

[0021] Under reflux for 24h;

[0022] (2) After the completion of the reaction, stand at room temperature in the dark for 2 days, remove the precipitate by suction filtration, dry the filtrate by rotary evaporation, use 2 mL of Φ=20% NaOH-methanol solution for alkaline hydrolysis at 80 degrees Celsius for 1 hour, and dry the solution by rotary evaporation;

[0023] (3) Dissolve in 2 mL of double-distilled water, adjust the pH to 3.5 with 2 mol / L sulfuric acid, then repeatedly extract with 2-3 mL of ethyl acetate for 5 times, discard the water phase, filter once with saturated saline, and add anhydrous sulfuric acid Sodium drying, after suction filtration, the solution was rotary evaporated to dryness, and the yellow oil collected...

Embodiment 2

[0031] A preparation method of zearalanol complete antigen, comprising the following steps:

[0032] (1) Weigh 2 mg of ZER and dissolve it in 3 mL of acetone, add excess potassium carbonate 10 mg and 1 microliter of ethyl 4-bromobutyrate, at 60 ° C

[0033] Under reflux for 24h;

[0034] (2) After the completion of the reaction, stand at room temperature in the dark for 2 days, remove the precipitate by suction filtration, dry the filtrate by rotary evaporation, use 2 mL of Φ=20% NaOH-methanol solution for alkaline hydrolysis at 80 degrees Celsius for 1 hour, and dry the solution by rotary evaporation;

[0035] (3) Dissolve in 2 mL of double-distilled water, adjust the pH to 3.5 with 2 mol / L sulfuric acid, then repeatedly extract with 2-3 mL of ethyl acetate for 5 times, discard the water phase, filter once with saturated saline, and add anhydrous sulfuric acid Sodium drying, after suction filtration, the solution was rotary evaporated to dryness, and the yellow oil collected...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of zeranol complete antigen. The preparation method is characterized by comprising the following steps: weighing 2mg by weight of ZER and dissolving into 3mL acetone, adding 10mg by weight of potassium carbonate and 1mu lof ethyl 4-bromobutyrate, and performing reflux for 24h at 60 DEG C. The antigen is high in valence and strong in sensitivity; and the preparation method is simple to operate, capable of being used for industrial production, and strong in practicability.

Description

technical field [0001] The invention relates to a preparation method of zearallol complete antigen. Background technique [0002] Zearalenol (Zeranol, ZER), also known as "right-ring tetradecone phenol", is the reduction product of the secondary metabolite Zearalenone (Zearalenone, ZEN) produced by Gibberella zebras during growth. Since ZEN is widely present in important crops such as corn, wheat, and sorghum, zearalanol may contaminate food and feed, and eventually lead to its residue in animal products. In addition, because ZER can improve the conversion rate of cattle and sheep feed and increase the lean meat percentage of cattle and sheep carcasses, it was once widely used as a growth promoter for cattle and sheep in the form of implantation. Although most of ZER will be metabolized in the body, some of it will remain at the implantation site. ZER and its metabolites have estrogen-like effects, can cause abortion, stillbirth, and even infertility in animals, and also h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K14/77
CPCC07K19/00C07K14/765C07K14/77
Inventor 郝青
Owner SHAANXI YUHANG ELECTRONICS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap