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A strain of Lactobacillus plantarum zn-3 and its application

A technology of Lactobacillus plantarum and ZN-3, which is applied in the fields of application, bacteria, and microorganisms, can solve the problems that the antibacterial effect has not been reported, and the characteristics of different strains are not the same, and the characteristics are not the same, so as to promote the growth of livestock and poultry , Promote intestinal peristalsis, increase resistance

Active Publication Date: 2018-05-18
WUHAN KEYUAN BIOLOGICAL DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some studies have also found that the characteristics of different strains are not the same, and the effects of different strains of the same strain are not the same. For the antibacterial effect of Lactobacillus plantarum, the current main research is on E. coli and Salmonella, Streptococcus suis, pig The bacteriostatic effects of Erysipelothrix, Haemophilus parasuis, porcine infectious pleuropneumonia and Basilicum have not been reported yet

Method used

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  • A strain of Lactobacillus plantarum zn-3 and its application
  • A strain of Lactobacillus plantarum zn-3 and its application
  • A strain of Lactobacillus plantarum zn-3 and its application

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Experimental program
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Effect test

Embodiment 1

[0033] Isolation and identification of Lactobacillus plantarum ZN-3:

[0034] 1. Isolation of Lactobacillus plantarum strains

[0035] Take Chinese herbal medicine fermented feed from an ecological pig farm in Guangxi, weigh 10g of fermented feed, add it to 90ml sterile single distilled water, culture at 25°C, 200rpm shaker for 30min, and serially dilute 10 times to 10 -7 , select an appropriate dilution and spread it on the lactic acid bacteria selective medium MRS medium, culture anaerobically at 35°C for 48-72h, pick a single colony on the plate, streak and purify it on the MRS medium plate multiple times, and aerobically culture at 35°C cultured to obtain pure colonies. Colony morphology observation and Gram staining were performed on the purified strains, and Gram-positive bacilli were selected. The colony diameter of the bacterial strain of the present invention is 1-2mm on the MRS medium, milky white, convex, circular, smooth surface, and facultative anaerobic. The s...

Embodiment 2

[0043] Bacteriostatic properties of Lactobacillus plantarum ZN-3

[0044] Clinically isolated pathogenic strains were used as indicator bacteria, which were:

[0045] Streptococcus suis type 2 SC19, Haemophilus parasuis SH0165, Actinobacillus pleuropneumoniae serotype 7 GZ9903, Escherichia coli 62 # (Man Xiaoying, Wu Bin, etc. Isolation, identification and characterization of porcine enterohaemorrhagic Escherichia coli. Chinese Journal of Zoonoses, 2009,25(10):984-987.), Pasteurella multocida HN06 (Liu W etc, Complete genome sequence of Pasteruella multocida HN06, a toxigenic strain of serogroupD.journal of Bacteriology, 2012Jun, 194(12):3292-3293), Erysipelothrix suis CY0160 (Wang yaetc.iTRAQ-based quantitative proteomic analysis potential virulencefactors of Erysipelothrix rhuriopathiae.Journal of Proteomics 160(2017):28-37.), Staphylococcus aureus J9(Xu haiyang etc.Evaluation of a novel chimeric B cellepitope-based vaccine against mastitis induced by either Streptococcus a...

Embodiment 3

[0060] Comparison of antibacterial effects of Lactobacillus plantarum ZN-3 and antibiotics on Escherichia coli and Staphylococcus aureus

[0061] Staphylococcus aureus J9 (Gram-positive bacteria) and Escherichia coli 62 # (Gram-negative bacteria) was used as an indicator bacterium, and the difference in the bacteriostatic effect of Lactobacillus plantarum ZN-3 and antibiotics on Gram-positive and negative bacteria was compared. The antibiotics used were penicillin, ampicillin, ceftriaxone (junbizhi), amikacin, gentamicin, kanamycin, neomycin, doxycycline, ofloxacin, cyclo Profloxacin, polymyxin B. The drug-sensitive paper was purchased from Hangzhou Microbiology Reagent Co., Ltd. Use NA as the plate medium for the antibacterial experiment.

[0062] Pick a single colony of Lactobacillus plantarum (Lactobacillus plantarum) ZN-3 and inoculate it in MRS liquid medium, cultivate it at 100 rpm at 35°C for 24 hours, and use it as the fermentation medium for ZN-3 antibacterial expe...

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Abstract

The invention discloses a strain of Lactobacillus plantarum ZN-3 and its application. The Lactobacillus plantarum ZN-3 provided by the invention is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is: CCTCC NO: M 2017286. The Lactobacillus plantarum ZN-3 provided by the present invention has the characteristics of acid resistance and bile salt resistance, can colonize the intestinal tract through the stomach, and has antibacterial effect on animal-derived pathogenic bacteria, including Gram-negative bacteria: large intestine bacillus, Salmonella, Pastobacterium multocida, Erysipelothrix suis, Haemophilus parasuis, porcine infectious pleuropneumonia, also includes Gram-positive bacteria: Streptococcus suis, Staphylococcus aureus, Staphylococcus epidermidis. Compared with antibiotics, it has a good antibacterial effect on pathogenic bacteria with strong drug resistance.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Lactobacillus plantarum (Lactobacillus plantarum) ZN-3 isolated from Chinese herbal medicine fermented feed, and also relates to the effect of the Lactobacillus plantarum ZN-3 on pathogenic animal sources. The inhibitory effect of pathogenic bacteria, and the application of probiotics in livestock and poultry breeding as prevention and treatment of animal intestinal diseases. Background technique [0002] Feed antibiotics are an antibiotic drug that can protect animal health and improve the production performance of livestock and poultry. Antibiotics have been widely used as feed additives in the livestock and poultry industry. With the emergence of problems such as the impact on human health, many countries have restricted the use of antibiotics for animal feed additives, trying to avoid the harm of antibiotics for feeding, especially in the past decade, the restrictions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A61K35/747A61P31/04A61P1/12A61P37/04C12R1/25
CPCA61K35/747A23K10/18C12N1/205C12R2001/25Y02A40/70
Inventor 金梅林杨丽康超滑亚锋王杨波马海军
Owner WUHAN KEYUAN BIOLOGICAL DEV CO LTD
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