Lactobacillus Plantarum ZN-3 and application thereof
A technology of Lactobacillus plantarum, ZN-3, applied in the application, bacteria, microorganisms and other directions, can solve the problems of different, bacteriostatic effect is not reported, the characteristics of different strains are not the same, etc., to increase resistance, The effect of promoting the growth of livestock and poultry and promoting intestinal peristalsis
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[0032] Example 1:
[0033] Isolation and identification of Lactobacillus plantarum ZN-3:
[0034] 1. Isolation of Lactobacillus plantarum strains
[0035] Take the Chinese herbal medicine fermented feed from an ecological pig farm in Guangxi. Weigh 10g of fermented feed and add it to 90ml of sterile mono-distilled water, culture at 25℃, 200rpm shaker for 30min, 10 times serial dilution to 10 -7 Select the appropriate dilution and spread it on the lactic acid bacteria selective medium MRS medium, cultivate it anaerobicly at 35℃ for 48-72h, pick a single colony on the plate, streak it on the MRS medium plate for multiple times and purify it with aerobic at 35℃ Cultivate to obtain pure colonies. Observe the colony morphology and Gram stain of the purified strains, and select Gram-positive bacilli. The bacterial strain of the invention has a colony diameter of 1-2 mm on the MRS medium, milky white, convex, round, smooth surface, and facultative anaerobic. The strain is Gram-positive, ...
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[0042] Example 2:
[0043] Bacteriostasis of Lactobacillus plantarum ZN-3
[0044] Taking clinically isolated pathogenic strains as indicator bacteria, they are:
[0045] Streptococcus suis type 2 SC19, Haemophilus parasuis SH0165, Actinobacillus pleuropneumoniae serotype 7 GZ9903, Escherichia coli 62 # (Man Xiaoying, Wu Bin, et al. Isolation, identification and characteristics of porcine enterohemorrhagic Escherichia coli. Chinese Journal of Zoonoses, 2009, 25(10): 984-987.), Basillus multocida HN06 (Liu W etc,Complete genome sequence of Pasteruella multocida HN06,a toxigenic strain of serogroup D.journal of Bacteriology,2012Jun,194(12):3292-3293), Erysipelas CY0160(Wang yaetc.iTRAQ-based quantitative proteomic analysis reveals) potential virulencefactors of Erysipelothrix rhuriopathiae.Journal of Proteomics 160(2017):28-37.), Staphylococcus aureus J9(Xu haiyang etc.Evaluation of a novel chimeric B cellepitope-based vaccine against mastitis induced by either Streptococcusagalactiae...
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[0059] Example 3:
[0060] Comparison of antibacterial effects of Lactobacillus plantarum ZN-3 and antibiotics on Escherichia coli and Staphylococcus aureus
[0061] Respectively with Staphylococcus aureus J9 (Gram-positive bacteria) and E. coli 62 # (Gram-negative bacteria) is an indicator bacteria. Compare the difference of the antibacterial effects of Lactobacillus plantarum ZN-3 and antibiotics on Gram-positive and Gram-negative bacteria. The antibiotics used were penicillin, ampicillin, ceftriaxone (bacteriostatic), amikacin, gentamicin, kanamycin, neomycin, doxycycline, ofloxacin, cycloheximide Profloxacin, Polymyxin B. The drug sensitive paper was purchased from Hangzhou Microbial Reagent Co., Ltd. Use NA as the antibacterial experiment plate medium.
[0062] A single colony of Lactobacillus plantarum ZN-3 was selected and inoculated into MRS liquid medium, and cultured at 35°C at 100 rpm for 24 hours as the fermentation broth for ZN-3 antibacterial experiment. The content ...
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