Lactobacillus Plantarum ZN-3 and application thereof

A technology of Lactobacillus plantarum, ZN-3, applied in the application, bacteria, microorganisms and other directions, can solve the problems of different, bacteriostatic effect is not reported, the characteristics of different strains are not the same, etc., to increase resistance, The effect of promoting the growth of livestock and poultry and promoting intestinal peristalsis

Active Publication Date: 2017-11-03
WUHAN KEYUAN BIOLOGICAL DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some studies have also found that the characteristics of different strains are not the same, and the effects of different strains of the same strain are not the same. For the antibacterial effect of Lactobacillus planta

Method used

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  • Lactobacillus Plantarum ZN-3 and application thereof
  • Lactobacillus Plantarum ZN-3 and application thereof
  • Lactobacillus Plantarum ZN-3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1:

[0033] Isolation and identification of Lactobacillus plantarum ZN-3:

[0034] 1. Isolation of Lactobacillus plantarum strains

[0035] Take the Chinese herbal medicine fermented feed from an ecological pig farm in Guangxi. Weigh 10g of fermented feed and add it to 90ml of sterile mono-distilled water, culture at 25℃, 200rpm shaker for 30min, 10 times serial dilution to 10 -7 Select the appropriate dilution and spread it on the lactic acid bacteria selective medium MRS medium, cultivate it anaerobicly at 35℃ for 48-72h, pick a single colony on the plate, streak it on the MRS medium plate for multiple times and purify it with aerobic at 35℃ Cultivate to obtain pure colonies. Observe the colony morphology and Gram stain of the purified strains, and select Gram-positive bacilli. The bacterial strain of the invention has a colony diameter of 1-2 mm on the MRS medium, milky white, convex, round, smooth surface, and facultative anaerobic. The strain is Gram-positive, ...

Example Embodiment

[0042] Example 2:

[0043] Bacteriostasis of Lactobacillus plantarum ZN-3

[0044] Taking clinically isolated pathogenic strains as indicator bacteria, they are:

[0045] Streptococcus suis type 2 SC19, Haemophilus parasuis SH0165, Actinobacillus pleuropneumoniae serotype 7 GZ9903, Escherichia coli 62 # (Man Xiaoying, Wu Bin, et al. Isolation, identification and characteristics of porcine enterohemorrhagic Escherichia coli. Chinese Journal of Zoonoses, 2009, 25(10): 984-987.), Basillus multocida HN06 (Liu W etc,Complete genome sequence of Pasteruella multocida HN06,a toxigenic strain of serogroup D.journal of Bacteriology,2012Jun,194(12):3292-3293), Erysipelas CY0160(Wang yaetc.iTRAQ-based quantitative proteomic analysis reveals) potential virulencefactors of Erysipelothrix rhuriopathiae.Journal of Proteomics 160(2017):28-37.), Staphylococcus aureus J9(Xu haiyang etc.Evaluation of a novel chimeric B cellepitope-based vaccine against mastitis induced by either Streptococcusagalactiae...

Example Embodiment

[0059] Example 3:

[0060] Comparison of antibacterial effects of Lactobacillus plantarum ZN-3 and antibiotics on Escherichia coli and Staphylococcus aureus

[0061] Respectively with Staphylococcus aureus J9 (Gram-positive bacteria) and E. coli 62 # (Gram-negative bacteria) is an indicator bacteria. Compare the difference of the antibacterial effects of Lactobacillus plantarum ZN-3 and antibiotics on Gram-positive and Gram-negative bacteria. The antibiotics used were penicillin, ampicillin, ceftriaxone (bacteriostatic), amikacin, gentamicin, kanamycin, neomycin, doxycycline, ofloxacin, cycloheximide Profloxacin, Polymyxin B. The drug sensitive paper was purchased from Hangzhou Microbial Reagent Co., Ltd. Use NA as the antibacterial experiment plate medium.

[0062] A single colony of Lactobacillus plantarum ZN-3 was selected and inoculated into MRS liquid medium, and cultured at 35°C at 100 rpm for 24 hours as the fermentation broth for ZN-3 antibacterial experiment. The content ...

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Abstract

The invention discloses Lactobacillus plantarum ZN-3 and application thereof. The Lactobacillus plantarum ZN-3 provided herein is collected in CCTCC (China Center for Type Culture Collection) under CCTCC NO: M 2017286. The Lactobacillus plantarum ZN-3 provided herein features acid tolerance and cholate tolerance, can be implanted to intestines via the stomach to inhibit zoonotic pathogens, including gram-negative bacteria that includes Escherichia coli, salmonella, Pasteurella multocida, Erysipelothrix rhuriopathiae, Haemophilus parasuis and porcine infectious pleuropneumonia, as well as gram-positive bacteria that include Streptococcus suis, Staphylococcus aureus, and Staphylococcus epidermidis. Compared with antibiotics, the Lactobacillus plantarum ZN-3 has good antibacterial effect for pathogenic bacteria having high drug tolerance.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Lactobacillus plantarum (Lactobacillus plantarum) ZN-3 isolated from Chinese herbal medicine fermented feed, and also relates to the effect of the Lactobacillus plantarum ZN-3 on pathogenic animal sources. The inhibitory effect of pathogenic bacteria, and the application of probiotics in livestock and poultry breeding as prevention and treatment of animal intestinal diseases. Background technique [0002] Feed antibiotics are an antibiotic drug that can protect animal health and improve the production performance of livestock and poultry. Antibiotics have been widely used as feed additives in the livestock and poultry industry. With the emergence of problems such as the impact on human health, many countries have restricted the use of antibiotics for animal feed additives, trying to avoid the harm of antibiotics for feeding, especially in the past decade, the restrictions...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A61K35/747A61P31/04A61P1/12A61P37/04C12R1/25
CPCA61K35/747A23K10/18C12N1/205C12R2001/25Y02A40/70
Inventor 金梅林杨丽康超滑亚锋王杨波马海军
Owner WUHAN KEYUAN BIOLOGICAL DEV CO LTD
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