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A kind of lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and animals, applied in the field of microorganisms, can solve problems such as bacterial food poisoning, reduction of reproductive productivity of livestock and poultry, aggravation of morbidity or mortality of livestock and poultry

Active Publication Date: 2021-12-03
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The clinical symptoms of Salmonella infection of livestock and poultry include aggravating the morbidity or mortality of livestock and poultry, or reducing the reproductive productivity of livestock and poultry; it may also be asymptomatic carrier state, and human consumption of these contaminated livestock products may cause serious bacterial infections. food poisoning

Method used

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  • A kind of lactobacillus plantarum and application thereof
  • A kind of lactobacillus plantarum and application thereof
  • A kind of lactobacillus plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Bacterial Strain Isolation and Purification

[0034] Lactobacillus plantarum PA01 was found in lactic acid bacteria isolated from traditional kimchi soaking solution in Yangling, Shaanxi.

[0035] Take 50g of the fermented kimchi purchased under aseptic conditions, and carry out 10-fold gradient dilution with a PBS solution with a pH of 7.4, and the dilution gradient is 10 2 ~10 6 , after the dilution was completed, 200 μL each was spread on the MRS solid medium (containing 2.0% CaCO 3 ), and cultured upside down in an anaerobic environment at 37°C for 48h. Then, single colonies with different sizes and shapes of calcium-dissolving circles were picked and inoculated on MRS liquid medium, and cultivated overnight at 37°C. Mix the liquid medium evenly, use an inoculation loop to dip a small amount of bacterial liquid in the ultra-clean workbench and streak it onto the MRS solid medium, and culture it upside down in an anaerobic environment at 37°C for 36-48 h...

Embodiment 2

[0037] The detection of the antibacterial ability of embodiment 2 lactobacillus plantarum PA01

[0038] After shaking the pathogenic bacteria for 10 hours, inoculate the LB solid medium at about 45°C with an inoculum of 0.1%; place the sterilized and dried Oxford cup in a petri dish; add about 25mL of LB solid medium containing pathogenic bacteria; after standing at room temperature for 1 hour, use sterilized tweezers to hold the Oxford cup without destroying the structure of the culture hole, and then place it in the ultra-clean workbench at room temperature for 1 hour; add 200 μL of Lactobacillus cultured for 20 hours, after the bacteria were completely absorbed at room temperature, cultured overnight at 37°C, the diameter of the inhibition zone was measured, and the antibacterial effect of Lactobacillus was indicated by the diameter of the inhibition zone.

[0039] When Escherichia coli (S.aureus ATCC 29213), Salmonella (Salmonella enteritidis (S.Enteritidis ATCC 13076) and...

Embodiment 3

[0043] Example 3 Detection of Lactobacillus plantarum PA01 resistance to gastrointestinal environment

[0044] Acid resistance test

[0045] Bring the concentration to about 10 8 ~10 9 Add 150μL of CFU / mL Lactobacillus plantarum suspension into 4.85mL acidic MRS liquid medium with pH 2.5; after mixing the medium, culture it statically in a sterile anaerobic environment at 37°C for 3 hours; suspend Lactobacillus plantarum PA01 10-fold dilution to 10 5 ~10 6 CFU / mL, add 500 μL of Lactobacillus plantarum suspension to the petri dish respectively; pour the MRS solid medium cooled to about 45°C into the petri dish and mix well, let it cool naturally for 30 minutes and place it in an anaerobic sterile environment at 37°C Cultured in medium for 48h, the concentration of Lactobacillus plantarum was detected to be 10 8.41 CFU / mL, and maintain normal culture 10 8.65 CFU / mL was almost the same activity.

[0046] Bile salt resistance test

[0047] Bring the concentration to about ...

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Abstract

The invention discloses a plant lactobacillus and an application thereof. The plant lactobacillus (Lactobacillus plantarum) has a bacterial strain of PA01 and a preservation number of CGMCC No.15660. The Lactobacillus plantarum has high safety and strong tolerance, can colonize, grow and reproduce in the intestinal tract, regulate the balance of intestinal flora, and exert a probiotic effect, and can be used to prepare food or feed additives, and regulate intestinal diseases of animals. Agents, animal antibiotic substitutes, immunomodulators, pathogenic bacteria inhibitors, and can also be used to improve the production performance of broilers, with good effects, safety and no side effects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a plant lactobacillus and an application thereof. Background technique [0002] Salmonellosis is an important zoonotic infectious disease between humans and animals (poultry). The pathogenic Salmonella belongs to the family Enterobacteriaceae and is a Gram-negative enterobacteriaceae. The clinical symptoms of Salmonella infection of livestock and poultry include aggravating the morbidity or mortality of livestock and poultry, or reducing the reproductive productivity of livestock and poultry; it may also be asymptomatic carrier state, and human consumption of these contaminated livestock products may cause serious bacterial infections. Sexual food poisoning. Reducing the amount of Salmonella in the digestive tract of poultry during farming will reduce the risk of Salmonella entering the meat processing plant. [0003] Since the addition of antibiotic growth p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/75A61K35/747A61P1/00A61P31/04A61P37/02C12R1/25
CPCA61K2035/115A23K10/18A23K50/75A61P1/00A61P31/04A61P37/02C12N1/205C12R2001/25
Inventor 杨欣龙芳羽杨小军孙青竹任周正王晗王方圆
Owner NORTHWEST A & F UNIV
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