Kit for detecting circulating antigen indirect hemagglutination of schistosomiasis and manufacturing method thereof

A technology of schistosomiasis and kits, applied in the field of bioengineering, can solve problems such as inability to distinguish past infection from current infection, low sensitivity of circulating antigens, and inability to conduct curative effect assessment, achieving considerable economic and social benefits, easy promotion, high The effect of practical value
CN101893628AInactive Publication Date: 2010-11-24STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
Publication Date
2010-11-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a kit for detecting circulating antigen indirect hemagglutination of schistosomiasis and a manufacturing method thereof. The kit is composed of freeze-drying anti-SEA-IgY sensitized erythrocyte, sensitized erythrocyte diluent, sample diluents, positive comparison products and negative comparison products. The method for manufacturing the kit for detecting circulating antigen indirect hemagglutination of schistosomiasis comprises the steps of preparing a schistosoma japonica soluble antigen, performing antigen immunization and collecting eggs, extracting and purifying specific anti-SEA-IgY, preparing hydroformylated red cells, preparing tanned red cells, preparing a specific anti-SEA-IgY antibody sensitized erythrocyte, and preparing freeze-drying red cells. The kit in the invention has the advantages of simple and convenient operation, rapidness, high sensibility, strong specificity and good repeatability.
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Description

Technical field:

[0001] The invention belongs to the field of bioengineering, and in particular relates to a kit, in particular to an indirect hemagglutination kit for detecting schistosome circulating antigens and a manufacturing method thereof. Background technique:

[0002] 1. The importance of immunodiagnosis in the prevention and treatment of schistosomiasis japonicum

[0003] Schistosomiasis is a zoonotic parasitic disease widely prevalent in tropical and subtropical regions. About 200 million people in 76 countries and regions around the world are infected, and 600 million people are threatened by infection. my country is an endemic area of ​​schistosomiasis japonicum, which seriously affected people's normal production and life in the early days of the founding of the People's Republic of China. After more than 50 years of prevention and control, remarkable achievements have been made. By 2003, the total number of schistosomiasis patients in China was 843,007, which...

Claims

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