32 results about "Circulating antigen" patented technology

The invention provides an Echinococcus multilocularis circulating antigen dot immunogold filtration kit and a preparation method, relating to an Echinococcus multilocularis circulating antigen detection reagent. The kit is provided with a detection plate, a gold-marked antibody for resisting an Echinococcus multilocularis circulating antigen, and a washing solution; and the detection plate is provided with a carrier medium, a micro-pore filtration membrane, a water absorbing medium, an Echinococcus multilocularis circulating antigen detection point and a contrasting point. The preparation method comprises the following steps of: preparing the Echinococcus multilocularis circulating antigen; preparing the antibody for resisting the Echinococcus multilocularis circulating antigen; preparing colloidal gold; marking the colloidal gold and the antibody for resisting the Echinococcus multilocularis circulating antigen; and preparing the dot immunogold filtration kit. The Echinococcus multilocularis circulating antigen dot immunogold filtration kit can be used for detecting the Echinococcus multilocularis circulating antigen in samples including whole blood, blood serum, blood plasma and the like. When the Echinococcus multilocularis circulating antigen dot immunogold filtration kit is used for detecting, the needed sample amount is extremely small, a special instrument is not needed and a result can be directly judged and read by eyes; and the Echinococcus multilocularis circulating antigen dot immunogold filtration kit has the advantages of simplicity and rapidness in detection, strong specificity, high flexibility, accuracy and reliability, low cost and wide application.

The invention discloses an anti-schistosoma japonicum Sj14-3-3 protein monoclonal antibody and application thereof in immune diagnosis, and belongs to the technical field of immune diagnosis of parasitic diseases. The monoclonal antibody against recombinant Sj14-3-3 protein is generated by hybridoma JYQ5C6-1 which has the collection number of CCTCCC201118. The monoclonal antibody against recombinant Sj14-3-3 protein is applied to the detection of an antibody used by schistosoma infected patients or circulating antigen Sj14-3-3 protein in animal blood in schistosoma infected immune diagnosis. The prepared anti-Sj14-3-3 protein molecular specific monoclonal antibody can be used for establishing various immunological diagnosis methods for detecting anti-Sj14-3-3 protein circulating antigens. The time fluorescence resolution method for detecting circulating antigen Sj14-3-3 protein, established by using the anti-Sj14-3-3 protein specific monoclonal antibody, can further improve the accuracy and the positive rate of detecting the schistosoma circulating antigen Sj14-3-3 protein, and has good application prospect.

The invention relates to a double antibody sandwich method for detecting circulating antigen for angiostrongylus cantonensis and an enzyme-linked immunosorbent assay kit thereof, which belong to the technical field of enzyme immunoassay. The enzyme-linked immunosorbent assay kit for detecting the antigen for the angiostrongylus cantonensis comprises an ELIAS plate enveloped by an envelope antigen,and an enzyme marker, wherein the envelope antigen and the enzyme marker are both monoclonal antibodies for an angiostrongylus cantonensis protein secretion. The detection specificity of the kit reaches 100 percent; the precision (coefficient of variation C.V) is 8.54 percent; and the kit has simple and convenient operation, is rapid, can finish a one-step detection method within 40 minutes, andcan realize the multi-sample diagnosis and curative effect evaluation of angiostrongylus cantonensis.

The invention provides a kit for detecting the circulating antigen of oriental schistosomiasis, comprising streptavidin-coated magnetic beads, an oriental schistosomiasis resistant soluble egg antigen polyclonal antibody marked by biotin, an oriental schistosomiasis resistant soluble egg antigen specificity monoclonal antibody marked by an electrochemiluminescence reagent, a cleaning solution, a sample diluent, schistosomiasis positive serum, schistosomiasis negative serum and a co-reaction reagent. The invention further provides a method for detecting the circulating antigen of oriental schistosomiasis. In the invention, the kit for detecting the circulating antigen of oriental schistosomiasis is simple, convenient and fast in operation, is suitable for the clinic diagnosis, the curative effect check and the epidemiological investigation at the departments of hospital clinical laboratory, disease prevent and control center, community healthy center and the like, and has very high use value; and the double-antibody sandwich method for detecting the circulating antigen of oriental schistosomiasis has high sensitivity, strong specificity and good repeatability, and is suitable for the detection of batches of specimens and the curative effect check.

A rapid detection reagent of a toxoplasma circulating antigen immune colloidal gold marker comprises a sample pad, a bonding pad, a cellulose nitrate film, a water absorbent pad and a PVC back lining, wherein, one end of the PVC back lining is sequentially adhered with the sample pad, the bonding pad, the cellulose nitrate film and the water absorbent pad, the bonding pad is coated by a colloidal gold bonder with an anti-toxoplasma polyclonal antibody, the anti-toxoplasma polyclonal antibody and a toxoplasma metaboly secrete antigen are linearly coated on the cellulose nitrate film. The toxoplasma circulating antigen immune colloidal gold marker rapid detection reagent is prepared by preparing the anti-toxoplasma polyclonal antibody, preparing the toxoplasma metaboly secrete antigen, preparing the colloidal gold, preparing a colloidal gold marker of the anti-toxoplasma polyclonal antibody; the colloidal gold marker of the anti-toxoplasma polyclonal antibody is coated on the colloidal gold bonding pad, the anti-toxoplasma polyclonal antibody and the toxoplasma metaboly secrete antigen are linearly coated in a detection region and a stagnant control region of the cellulose nitrate film and the thorough drying and other processes are carried out. The rapid detection reagent of the toxoplasma circulating antigen immune colloidal gold marker can detect the toxoplasma circulating antigens of people and various animals, which has accurate sensitivity and simple operation, and being safe and stable.

In the invention, a method of hypodermic multi-point injection of specific antigens of blood flukes is used to immunize a hen, the specific IgY is extracted, purified and identified from egg yolks, and an ELISA diagnosis system IgY-ELISA is established by combining the monoclonal antibody technique. The invention establishes a method for detecting circulating antigens of blood flukes, which is novel, highly efficient, sensitive and specific and also has a curative effect evaluation action. Moreover, the invention also initially provides a novel technique and means for detecting other pathogensand diagnosing infectious diseases.

The invention provides a diagnostic kit for detecting Paragonimiasis, which contains a rabbit polyclonal antibody against the soluble antigen of Paragonia wescheri adult worm, an enzyme-labeled rabbit polyclonal antibody against the soluble antigen of Paragonimus westermani adult worm, Nitrocellulose membrane, bovine serum albumin, PBST washing solution, substrate chromogenic solution, positive control sample, negative control sample and polyethylene reaction plate. The invention also provides a preparation method of a diagnostic kit for detecting paragonimiasis. The invention adopts the double-antibody sandwich method Dot-ELISA to detect the circulating antigen of Paragonimus westermani, has high sensitivity and strong specificity, and can realize rapid diagnosis and curative effect assessment of Paragonimiasis.

The invention provides a kit for detecting circulating antigens of Agkistrodon acutinae using specific polyantibody, comprising rabbit anti-Aag-IgG, enzyme-labeled antibody (HRP-Aag-IgG), nitrocellulose film, 1 % bovine serum albumin in PBST solution, PBST washing solution, substrate chromogenic solution, positive and negative control sample composition and polyethylene reaction plate. The invention also provides a preparation method of a kit for detecting circulating antigens of Agkistrodon acutina by using specific polyantibody. The invention adopts the polyclonal antibody to measure the circulating antigen, which is beneficial to making early diagnosis and helping to judge the prognosis. When the parasites in the body die, the circulating antigens disappear quickly, which can be used for efficacy assessment. The kit of the invention has high sensitivity and strong specificity, and can realize rapid diagnosis, drug screening and curative effect assessment of Agkistrodon acutinae ligamentiworm disease.

The invention discloses a method for detecting a trichinella circulating antigen by utilizing IgY-McAb sandwich ELISA (enzyme-linked immnuo sorbent assay). With an anti-trichinella muscle larval ES antigen egg yolk antibody IgY as a capture antibody and an anti-trichinella ES antigen McAb as a detection antibody, the preparation method of the IgY comprises the following steps of: adding the trichinella muscle larval into a culture medium to carry out sterile culture, purifying and dialyzing supernate and then concentrating and drying to obtain the ES antiagen, determining protein concentration and then immunizing roman legehenne by utilizing the ES antigen, collecting the IgY from the produced egg yolk by adopting a saturate ammonium sulphate precipitation method, purifying and determining concentration, and sub-packaging for later use; and the preparation method of the McAb comprises the following steps of: establishing a hybridoma cell strain secreting the anti-trichinella muscle larval ES antigen, cloning by adopting a limiting dilution method, purifying McAb by applying an octanoic acid-ammonia sulphate method, determining the concentration and sub-packaging for later use. The invention has the advantage that a new method which has high sensitivity and specificity and has wide application prospect is provided for early detection of trichinella CAg in blood serum of an infected animal.

The invention relates to a method for detecting a schistosome circulating antigen, which comprises the following steps of: preparing a schistosome soluble egg antigen (SEA) resistant specific IgY polyclonal antibody; preparing an enzyme-labeled schistosome SEA resistant monoclonal antibody by adopting a hybridoma technology; selecting a polyclonal antibody for coating a solid-phase carrier; selecting a schistosome SEA resistant monoclonal antibody for preparing an enzyme composition; forming a schistosome SEA resistant specific IgY polyclonal antibody-circulating antigen-enzyme-labeled schistosome SEA resistant monoclonal antibody sandwich complex; and adding a substrate for developing and indicating. The invention also provides an enzyme-linked immune kit for detecting the schistosome circulating antigen. The method for detecting schistosome circulating antigen has the advantages of high sensitivity, high specificity and high repeatability and is respectively suitable for the detection of mass specimens and curative effect assessment.

The invention discloses a toxoplasma circulating antigen double antibody sandwich ELISA (Enzyme-Linked Immuno Sorbent Assay) detection reagent and a preparation method. Toxoplasma MIC10 is a protein secreted by toxoplasma when invading a host cell and has important actions in invasion and proliferation of the toxoplasma. In the invention, amplification and expression at different areas are carried out on an MIC10 gene to obtain recombinant fusion proteins MIC10A, MIC10B and MIC10C, the purified recombinant fusion proteins MIC10A and MIC10B respectively immunize rabbits, are used for preparinga polyclonal antibody and are respectively used as a coated antibody and a detection antibody, a double antibody sandwich ELISA is established with purified MIC10C as a standard antibody to detect a toxoplasma circulating antigen in serum and has the advantages of strong specificity, strong sensitivity, strong reproducibility and strong stability, wherein y is equal to 0.0304Ln(x)-0.0567, R2 is equal to 0.9643, and the minimum detectable amount is 50pg/ml.

The invention relates to the field of immunology, and particularly relates to a B cell epitope polypeptide of a serine protease inhibitor (Ts-WM5) with high expression in the larvae phase of trichinamuscle, a hybridoma cell strain, a monoclonal antibody and application thereof. The hybridoma cell strain capable of secreting an anti-Ts-WM5 protein specific antibody is obtained, and the Ts-WM5 protein specific B cell epitope polypeptide recognized by the monoclonal antibody secreted by the hybridoma cell strain is identified, so that the B cell epitope polypeptide has important significance indiagnosis of trichinosis and has important significance in establishing sandwich ELSIA for competitive ELISA and detection of circulating antigens for detection of multiple hosts of trichina and development of subunit vaccines.

The invention relates to the field of immunology, in particular to B cell epitope polypeptide of a cysteine protease inhibitor (Ts-WN10) of trichina at an intestinal tract period, a hybridoma cell strain, a monoclonal antibody and an application of the B cell epitope polypeptide, the hybridoma cell strain and the monoclonal antibody. The hybridoma cell strain which can secrete Ts-WN10 protein resistant specific antibodies can be obtained, Ts-WN10 protein specific B cell epitope polypeptide recognized by the monoclonal antibody secreted by the hybridoma cell strain is identified, and the B cellepitope polypeptide, the hybridoma cell strain and the monoclonal antibody have important significance in diagnosis of trichina diseases, and have important significance in detection of trichina multiple hosts, by sandwich ELSIA which is used for establishing competitive ELISA and detecting a circulating antigen and development of a subunit vaccine.

An immunocolloidal gold test paper strip for detecting a pig toxoplasmosis circulating antigen and a preparing method thereof are provided. The colloidal gold immunochromatography technique is utilized to detect the circulating antigen in blood of pigs affected with toxoplasmosis. T line coating and gold marking pad preparation utilize two monoclonal antibodies resisting a toxoplasma gondii surface antigen SAG3 respectively. The test paper strip has no cross reaction with two types of pig cysticercus positive serum so that the test paper strip has high specificity. The test paper strip has biosecurity. Preparation of the gold marking pad and coating of a T line apply the two monoclonal antibodies resisting the toxoplasma gondii surface antigen SAG3, a goat anti-mouse antibody is coated by a C line, the antibodies are nontoxic, and the test paper strip does not cause environment pollution.

An immunochromatographic test strip for diagnosing kala-azar based on detection of circulating antigens comprises a sample mat, a gold labeling mat containing colloidal gold labeling probes, a cellulose membrane and a water absorption mat. A quality control line is arranged at the end, away from the gold labeling mat, of the cellulose membrane, a detection line is arranged on the portion, between the quality control line and the gold labeling mat, of the cellulose membrane, the detection line is composed of monoclonal antibodies of specific binding Leishmania donovani amastigote polypide antigens and generated by hybridomas, with the preservation number of CGMCC No.9240, of mice, the colloidal gold labeling probes are monoclonal antibodies of specific binding Leishmania donovani amastigote polypide antigens with different epitopes and generated by hybridomas, with the preservation number of CGMCC No.9239, of mice, and the quality control line is composed of secondary antibodies of the specific binding colloidal gold labeling probes or streptococcus G protein or staphylococcus aureus A protein. The immunochromatographic test strip is high in sensitivity and specificity, the overall sensitivity can reach 83%, and the specificity is 95%.

The invention discloses a colloidal gold test strip for detecting a lamb fasciola hepatica infection circulating antigen, and the prepared lamb fasciola hepatica infection colloidal gold test strip can be used for detecting the lamb fasciola hepatica infection circulating antigen and has the advantages of simplicity in operation, rapidness in detection, convenience in carrying and the like in the aspect of use. The detection result is visual, easy to understand and convenient to interpret, and the kit can be used for detecting early infection of fasciola hepatica, can distinguish current infection from previous infection, and is suitable for on-site detection of clinical samples. The anti-fasciola hepatica cathepsin L monoclonal antibody ensures the specificity of the detection method. The rabbit anti-fasciola hepatica cathepsin L polyclonal antibody improves the sensitivity of the detection method.

Methods for the diagnosis of visceral, cutaneous and canine leishmaniasis in a subject suspected of being infected with the parasitic protozoa Leishmania is disclosed. Disclosed are antibody-capture enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies to Leishmania parasite soluble antigens and antigen-capture ELISAs for the detection of Leishmania parasite soluble antigens in host samples. Also disclosed are immunodiagnostic kits for the detection of Leishmania parasite circulating antigens or IgM and IgG antibodies in a sample from subject having visceral, cutaneous or canine leishmaniasis. In these methods and kits, detection may be done photometrically or visually. The methods and kits also allow the visualization of Leishmania amastigotes or promastigotes in a sample.

Methods for the diagnosis of visceral, cutaneous and canine leishmaniasis in a subject suspected of being infected with the parasitic protozoa Leishmania is disclosed. Disclosed are antibody-capture enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies to Leishmania parasite soluble antigens and antigen-capture ELISAs for the detection of Leishmania parasite soluble antigens in host samples. Also disclosed are immunodiagnostic kits for the detection of Leishmania parasite circulating antigens or IgM and IgG antibodies in a sample from subject having visceral, cutaneous or canine leishmaniasis. In these methods and kits, detection may be done photometrically or visually. The methods and kits also allow the visualization of Leishmania amastigotes or promastigotes in a sample.