85results about How to "Enable early detection" patented technology

The invention relates to the technical field of plant protection, and discloses a method and device for early detecting crop pests based on multisensor information fusion. The method disclosed by the invention is used for detecting a crop pest condition through a model building stage, an information collecting stage, a characteristic quantity drawing stage and an information fusion and detection distinguishing stage. By using the method and the device, the shortage of purely depending on a single machine checking and monitoring method is overcome, relevant information of specific pests can beobtained from multiple aspects, multiple kinds of information can be fused for intelligent detection; and multiple kinds of information are subjected to demonstration check for mutual compensation byusing complementarity and redundancy of the information, and further the detecting and monitoring accuracy and efficiency of a field crop pest robot are improved.

The invention relates to an air-gap field detecting device of an alternating-current generator, comprising a detecting coil and a signal detecting and processing circuit, wherein the detecting coil is arranged on a rotor of the alternating-current generator and surrounds one zone on the inner surface of the stator, and the induced voltage of the detection coil is relevant to the strength of an air-gap field in the zone surrounded by the detecting coil, as well as the distribution condition and the changes of the air-gap field; the signal detecting and processing circuit is connected with the detecting coil and used for detecting and processing the induced voltage of the detecting coil; the signal detecting and processing circuit can be used for detecting and processing the fundamental waves or the harmonic waves of the induced voltage of the detecting coil, or detecting and comparing the induced voltages of different detecting coils, and the detection and processing result can be used for the monitoring of the states of the generator, including the faults of stator and rotor windings, the decentraction of a stator and a rotor, axial and opposite shift of the stator and the rotor, magnetism loss, overload, external short circuit and circuit breakage and the like; and the detection and processing result of signals can also be used for the operation control of the generator.

The invention relates to a historical operation data-based equipment alarm threshold calculation method. The method comprises the following steps that: step 1, the historical operation data of equipment are acquired; step 2, the historical operation data are preprocessed; step 3, the statistic indexes of the preprocessed historical data are calculated, wherein the indexes comprise a data average value and a data standard deviation; step 4, an alarm threshold value Alm is calculated through the data average value and the data standard deviation; and step 5, the actually-measured state parameterS of the equipment is monitored, the equipment gives out an alarm, and the alarm threshold value iteratively updated. According to the method of the invention the actual alarm threshold value of theequipment is determined based on the learning of the historical operation data of the equipment, so that automatic early warning against the faults of the equipment can be realized.

The invention discloses papillomavirus detection and a typing liquid chip, and a process which is used to the detection and typing papillomavirus. The liquid chip mainly comprises microsphere which is respectively enveloped with specific anti-tag label sequence and 7-10 T spacer arm sequence which is arranged between anti-tag label sequence and microsphere, wherein anti-tag label sequence is chosen from two or more than two sequences in SEQ ID NO. 25- SEQ ID NO.47, specific ASPE primer is respectively designed aiming at each typing HPV, ASPE primer is chosen from two or more than two sequence from SEQ ID NO.1-SEQ ID NO.23, and primer of target sequence which has mutant sites is amplified. The liquid chip improves the current liquid chip technology, which makes ribonucleotide probe microspheres which is prepared be suitable to different detection projects, largely increases fluorescent signal value which is detected, thereby further increasing sensitivity of the detection, strengthening signal-to-noise ratio, and making detection results more accurate and reliable.

The invention discloses a sea urchin-shaped hollow gold and silver alloy nano particle and a preparation method and application thereof. The method comprises the following steps: dissolving silver nitrate into water; heating the mixture of the silver nitrate and the water till boiling; adding trisodium citrate aqueous solution in the mixture and keeping boiling for 3-30 minutes to obtain a silver colloid; mixing chloroauric acid aqueous solution and the water; adding the silver colloid under the condition of agitation at the temperature of 15 DEG C; adding hemoporphyrin metalporphyrin aqueous solution and obtaining a reaction solution after 5 min reaction; performing centrifugalization of the reaction solution, washing deposit obtained via centrifugalization, and obtaining the sea urchin-shaped hollow gold and silver alloy nano particle. The diameter of the nano particle is 80 nm-300 nm, the hollow size is 20 nm-50 nm, the length of a stab is 10 nm-30 nm, the diameter of the stab is 10 nm. The preparation method is simple, low in cost, gentle in condition, short in time, easy in process control, and large in production capacity. The obtained nano particle can be well applied to organic molecule detection, earlier detection of tumour and photothermal treatment.

The invention relates to an indirect distributed denial of service attack defense method and an indirect distributed denial of service attack defense system based on a Web agency. A behavior characteristic of a proxy-to-server network flow is described by extracting the space-time local property of the proxy-to-server network flow; the interference of a small-probability large value on an available signal is restrained by a nonlinear mapping function; a normal behavior model of the proxy-to-server network is constructed through a hidden semi-markov model (HsMM); normal degree estimation, namely long-time behavior estimation and short-time behavior estimation, under different time scales is performed by using behavior indexes acquired by the model; as to an abnormal behavior sequence (HTTP request sequence), an attack response is implemented by adopting a soft control method; and the basis of the soft control represents an HsMM model parameter and a structure index which are used for performing a normal behavior. The parameter for describing the proxy-to-server network is the space-time local property which is irrelevant to the change of the Web content on a target server; and the detection property of the method is the nature property based on the agent network flow and irrelevant to the size of the attack flow. By the method, the attack response can be realized before the resources of the target server are used by the attack flow, so that early detection can be realized effectively.

The invention discloses a system and a method for oil field united station fire disaster pre-warning system based on video images. The system comprises a fire disaster pre-warning management computer and a fire disaster pre-warning controller connected with the fire disaster pre-warning management computer; the input end of the fire disaster pre-warning management computer is connected with a plurality of image type fire disaster detectors arranged in the monitored oil field united station. The method comprises the following steps: 1, acquiring and transmitting video images, 2, pre-processing the video images, 3, extracting characteristics, 4, acquiring a training sample, 5, constructing BP neural network model, 6, training BP neural network model, and 7, recognizing fire disaster and pre-warning. The system and the method have reasonable design, good real-time property, high sensitivity, high accuracy, high effectiveness and reliability, strong anti-jamming capability, strong practicability, good use effect, and high popularization and application values.

The invention discloses a cancer early detection chip based on microfluidic control technology, which comprises a circulating tumor cell rapid sorting module, a detection sample uniform mixing module,a mixed reagent quantitative wrapping module and a discrete droplet fluorescence detection module. The four modules are sequentially connected, the characteristics of circulating tumor cells carryingtumor information are utilized, the inertial microfluidic cell sorting technology, the chaotic microchannel mixing technology and the droplet quantitative packaging technology are combined, a set ofcancer cell detection chip which is composed of four modules: rapid sorting of circulating tumor cells, uniform mixing of detection samples, quantitative wrapping of mixed reagents and discrete droplet fluorescence detection, so as to realize rapid detection of circulating tumor cells from peripheral blood and complete detection of tumor cells.

The invention provides disaster relieving system and method. The disaster relieving system comprises a detecting subsystem and a disaster relieving subsystem; the detecting subsystem comprises a firstacquisition module used for acquiring disaster monitoring information in a protection zone and a disaster judgment module connected with the first acquisition module and used for judging whether a disaster happens or not according to the disaster monitoring information; and the disaster relieving subsystem comprises a disaster relieving execution module connected with the disaster judgment moduleand used for executing disaster relief when the disaster happens. The invention integrates early detection of disasters and automatic disaster relief into a whole, thereby improving the disaster preventing and relieving capacity of the disaster relieving system.

The invention relates to a kit for detecting colorectal cancer and precancerous lesions and a use method thereof. The invention provides the kit for detecting colorectal cancer and precancerous lesions, and the kit comprises a nucleic acid separation and purification reagent, a DNA sulfite conversion reagent, a KRAS gene mutation detection reagent, an SDC2 and SFRP2 gene methylation detection reagent and a fecal occult blood detection reagent, wherein the nucleic acid separation and purification reagent is used for separating and purifying human DNA in a fecal sample; and the DNA sulfite conversion reagent is used for converting the partial purified human DNA into sulfite for subsequent detection of the methylation level of SDC2 and SFRP2 genes. The sensitivity of the kit for detecting thecolorectal cancer can reach up to 90% or more and the specificity is about 85%, thereby realizing high-sensitivity and high-specificity detection of the colorectal cancer; and the kit provided by theinvention can detect most precancerous lesions of the colorectal cancer to realize ultra-early detection of the colorectal cancer, and can greatly reduce missed diagnosis and missed detection rates of clinical existing methods when being used for assisting clinical diagnosis.

The invention discloses an SNP molecular marker for detecting heterogenous cfDNA, a detecting probe based on SNP molecular marker design and a chip, a detecting device for heterogenous cfDNA, a kit and a detecting method. The heterogenous cfDNA in a receptor after kidney transplantation is quantified by the detecting chip and the detecting device, a basis is provided for detection of kidney transplantation rejection, noninvasive early detection with high sensitivity and high specificity on kidney transplantation is realized, and a kidney transplantation patient can be monitored continuously. An SNP molecular marker comprises an SNP site of a drug metabolizing gene, mutation information of a medication gene is provided for a kidney transplantation rejection patient, and individualized medication is realized. The invention further provides a single molecular marker joint. The single molecular marker joint is used for constructing a sequencing library to effectively remove repeated data and error introduced randomly in sequencing and PCR processes, the sequencing background noises are reduced, and the detection accuracy is improved.

The invention relates to interference photoelectric sensing smoke and fire detection method and device. The method includes: a. the light from the light source is divided into two beams with an optical splitter, one beam is emitted to the oscillation reference reflecting mirror, another beam is emitted to the target smoke sensing part; the scattering light generated by smoke and reflected light from the reference reflecting mirror are returned to the splitter to be superposed and carries on interference, and generates interference light signal; b. receives the interference signal with the photoelectric detector, and it is converted into interference photoelectric signal; c. the signal processing and analyzing device receive the interference signal, amplifies and processes them, judges if there has scattering light signal form fire smoke. The invention uses optical interference theory, upgrades the detection signal-to-noise ration and flexibility; the device needs not detection chamber and smoke absorbing device, it can be put into the open space to be detected, it can realizes the early detection to fire. The interference signal can be transmitted in fiber.

The invention relates to an early fire detection method which is used for detecting a fire and comprises the following steps: S1, acquiring a real-time monitoring video; S2, providing a single-frame detection network model, and using the single-frame detection network model to detect whether flame and / or smoke exists in the image at the current moment or not so as to obtain a first detection result; S3, providing a time sequence detection network model, and detecting a flame level and / or a smoke level in the video sequence before the current moment by using the time sequence detection networkmodel to obtain a second detection result; and S4, calculating a fire risk level by combining the first detection result and the second detection result. Thus, missed detection of fire disasters is prevented, and the accuracy of fire detection is improved. The invention also provides a computer readable medium. The invention also provides a detection system.

The invention discloses a drilling tool internal thread rotary detection device based on the magnetic memory effect. The drilling tool internal thread rotary detection device based on the magnetic memory effect comprises a centering structure assembly and a rotary detection assembly, wherein the centering structure assembly can enable the axis of the rotary detection assembly to be coincide with the axis of a threaded hole of a detected drilling tool joint, thereby enabling a magnetic memory sensor in the rotary detection assembly to keep a constant lift-off value in rotation measurement when abutting against the detected internal thread surface. Therefore, the drilling tool internal thread rotary detection device based on the magnetic memory effect has the characteristics of high detection precision, simple and convenient operation and the like.

The invention discloses an intelligent information fusion image type fire hazard detector comprising an outer shield, an optical lens and imaging assembly, and a video image and information fusion assembly; the optical lens and imaging assembly is connected with the video image and information fusion assembly, and both are arranged in the outer shield; the optical lens and imaging assembly sends shot image information to the video image and information fusion assembly so as to carry out information fusion calculation, thereby outputting detection results. The invention also discloses a detection information fusion method of the image type fire hazard detector; the image type fire hazard detector has high sensitivity, can realize early detection of the fire hazard, is excellent in accuracy, very low in false alarm rate, good in environment adaptability, and can stably and reliably operate; the detection information fusion method of the fire hazard detector is based on the intelligent information fusion method, can maximumly reduce false alarms and alarm failure phenomenon, thereby effectively improving accuracy and reliability of the image type fire hazard detector.

The invention provides a video-based shielded flame detecting method. The method comprises: step 101) analyzing the brightness variation between a present frame image and a last frame image and acquiring a communication area for different brightness, step 102) analyzing the variation trend of the communication area and acquiring the communication area having the variation trend meeting flicker feature as a candidate area, step 103) extracting the features of the candidate area, filtering false candidate area according to the features of the candidate area, using the remained candidate area as a flame detecting area and outputting, wherein the features comprise color feature and area edge change feature. The invention also provides a video-based shielded flame detecting device. Compared with the prior art of image flame detection, the method and the device of the invention can detect the shielded or secluded flame in a complex scene and realize the early detection for flame.

The invention relates to a detection method of sweet potato black rot pathogen, and belongs to the technical field of bioengineering. The method specifically comprises steps as follows: a pair of sweet potato black rot pathogen specific primers SPCPF and SPCPR is designed and synthesized; total DNA (deoxyribonucleic acid) of a to-be-tested sample is extracted and taken as a template, and the primers SPCPF and SPCPR are used for performing PCR (polymerase chain reaction) amplification; and an amplification product is detected through agarose gel electrophoresis. The method is simple, convenient, fast and sensitive, not only can be used for detecting pathogen mycelia but also can be used for detecting sick potato seedlings and pieces; the detection method can be used for performing early detection on sweet potato black rot and further can be used for effectively distinguishing similar diseases, such as a black nevus disease, soft rot and dry rot, of sweet potatoes; and the method has significance on early warning and propagation prevention and control of the sweet potato black rot pathogen.

The invention discloses a high-grade serous ovarian cancer recurrence risk prediction system based on imaging omics. A method comprises the steps of T1 weighted enhanced imaging omics processing, T2 weighted imaging omics processing and information fusion. The image omics processing mainly comprises the steps of three-dimensional tumor segmentation, image standardization, image omics feature extraction, feature normalization, feature screening, SMOTE resampling and classifier training; the information fusion is mainly used for fusing recurrence risk prediction probabilities output by T1 and T2image omics processing, so the risk prediction accuracy is further improved.

The invention provides a phomopsis asparagi LAMP (loop-mediated isothermal amplification) detection primer pair and a detection method thereof, which are specially used for specific detection of phomopsis asparagi, and belongs to the field of disease detection and biological technologies for crops. The phomopsis asparagi LAMP detection primer pair comprises outer primers F3 and B3 and inner primers FIP and BIP and has the sequence in SEQ ID NO.1-4. According to the phomopsis asparagi detection method established on the basis of the primer pair, green fluorescence or ladder bands with the LAMPfeatures can be observed through a chromogenic reaction or agarose gel electrophoresis detection after LAMP constant-temperature amplification. According to the LAMP detection primer pair and the detection method thereof, rapid, sensitive and accurate detection of plants infected with phomopsis asparagi can be realized in production practice, besides, the LAMP detection primer pair and the detection method can be used for early diagnosis of field diseases and monitoring and identification of germs and reliable technological and theoretical bases are provided for early warning and control of the phomopsis asparagi.

The invention provides a primer, detection method and kit for detecting human EGFR (Epidermal Growth Factor Receptor) gene T790M mutation. The detection primer provided by the invention can be combined with an EGFR gene conserved sequence to amplify a mutation sequence. The detection method provided by the invention can be combined with an amplified fragment by adopting a fluorescence signal detection group to send out a fluorescence signal; a blocking agent is specifically combined with a wild type sequence corresponding to a mutation site to inhibit wild type non-specific amplification. A competitive allele specific PCR (Polymerase Chain Reaction) amplification technology is adopted to establish the kit based on real-time fluorescence PCR and the EGFR gene T790M mutation can be accurately detected. The method provided by the invention has the advantages of simplicity in operation, easiness of reading results and high sensitivity, and can be used for detecting a sample containing 0.001 percent of the EGFR gene T790M mutation; ultrasensitive detection of the EGFR gene T790M mutation is realized.

The invention discloses a broad-leaved wood log internal quality assessment system, which obtains response signals by mainly performing an acoustic impact test on the log, and then analyzes the response signal to evaluate the health status inside the log. The specific scheme is that: using the acoustic impact test as a non-destructive assessment method to test defects such as decay and voids in the log; obtaining response signals through the acoustic impact test on the log; and then analyzing the response signals to assess the health status inside the log; which meets actual use requirements.

The invention discloses a human papillomavirus quick detection method, a liquid-phase chip and a kit. The liquid-phase chip comprises microsphere carriers, labeled molecules and nucleic acid capture probes aiming at different HPV types, the microsphere carriers are connected with coupling groups, the labeled molecules are available for independent detection, each capture probe comprises a specificnucleotide sequence and at least one coupling group, and each specific nucleotide sequence and target nucleic acid molecules coded by different types of HPV viruses form complementary pairs. By adoption of the method, accurate quantitative marking of the microsphere carriers can be realized, and stability and distinguishability in marking of the same type of microsphere carriers are remarkably improved. On the basis of the chip, the invention further provides a HPV detection method and a kit, 24 common HPV types can be detected in one time, the defect of detection omission in application of serology and immunohistochemical methods can be overcome while HPV detection efficiency is greatly improved, and early detection of the HPV viruses is realized.

The fire monitoring technology of the present invention is a fire differential analysis technology, adopts a color CCD camera system, uses the different reflections of the three primary colors of the color image on the early flame, performs differential calculations on the red and blue primary color components, binarizes, and performs differential calculations again, using the flame The growth rate of the area difference image is used to judge the abnormality of the fire, and the direct linear transformation technology is used to locate the fire space, which improves the reliability of fire detection. The existing closed-circuit television system can be used to realize the automatic monitoring, positioning and monitoring of the fire by the computer. Linkage saves.

The invention relates to a serum protein marker for the early diagnosis of lung cancer. The marker comprises CD44, SCC, CEA, CYFRA21-1, NNMT, CA125, NSE, FDG, TPS and GRP. The biological protein marker in the serum of a suspected patient is analyzed to realize the early diagnosis of lung cancer. The probability of transformation of a lung cyst into a malignant tumor can be sensitively detected by virtue of changes in the protein concentration of the biological marker, so that the time for the diagnosis and treatment of lung cancer is gained.

The invention provides an ELISPOT detection kit for detecting bovine brucellosis.The ELISPOT detection kit comprises a supporting medium, a capture antibody, a detection antibody, a negative control, a positive control and a specificity irritant, wherein the capture antibody is the first bovine gamma interferon monoclonal antibody, the detection antibody is the second marked bovine gamma interferon monoclonal antibody, and the specificity irritant is brucellin Br-PPD.A micropore filter membrane is arranged on a contact face of the supporting medium and the reagent.The capture antibody coats the supporting medium or the capture antibody and the supporting medium are placed respectively.The capture antibody and the detection antibody can be subjected to the antigen antibody combination effect together with bovine gamma interferon.According to the detection kit, the adopted monoclonal antibody excreted by hybrid tumor cell strains has the advantages of being high in active valence, good in specificity and strong in natural antigen affinity.Compared with a brucellosis antibody ELISA detection kit, the ELISPOT can achieve early detection of bovine brucellosis infection, false positive interference brought by a cross reaction is eliminated, and the good specificity is shown.

A detection reagent strip utilizing a recombinant fluorescent phycobiliprotein subunit comprises a non-fluorescence bottom plate, first non-fluorescence filter paper, a detection protein binding membrane, a fluorescent sample pad and second non-fluorescence filter paper, wherein a detection line and a control line are arranged on the detection protein binding membrane; a detection antibody is marked on the detection line; a control antibody is marked on the control line; the fluorescent sample pad comprises a glass fiber membrane and a mixture of a streptavidin blended phycobiliprotein subunit and a biotinylated antibody; the mixture covers the glass fiber membrane. In addition, the invention further provides a preparation method of the detection reagent strip utilizing the recombinant fluorescent phycobiliprotein subunit. The detection reagent strip utilizing the recombinant fluorescent phycobiliprotein subunit can perform qualitative and quantitative detection on antigens or antibodies of diseases such as tumor and microbial infection, and the recombinant fluorescent phycobiliprotein subunit used by the reagent strip is environmentally friendly. The preparation method is low in purification preparation cost and environmentally friendly.

The invention belongs to the field of immunodetection, and particularly discloses three kits for detecting human phosphorylated Tau protein and a preparation method thereof. According to the three detection kits disclosed by the invention, the monoclonal antibody 3G5 is used as a capture antibody, and the monoclonal antibody 4B1 is used as a detection antibody, or the monoclonal antibody 4B1 is used as a capture antibody, and the monoclonal antibody 3G5 is used as a detection antibody, wherein p-hTau396, 404 protein can be detected by the kit. The three detection kits constructed by using the monoclonal antibodies 3G5 and 4B1 are respectively suitable for detection in different scenes. The method can rapidly and accurately detect the human phosphorylated Tau, integrates the characteristics of specificity of the antibody, high sensitivity of ELISA, efficient enrichment of the immune nano magnetic beads, convenience and rapidness of the colloidal gold test strip and the like, and has the characteristics of accuracy, rapidness, high efficiency, specificity and sensitivity. Therefore, the method is suitable for rapid and sensitive early diagnosis of Alzheimer's disease, and is of great significance to timely and accurate guidance of medicine use for patients.

The invention relates to a molecular detection primer for banana cladosporium cucumerinum and a detection method and belongs to the technical field of crop disease detection and biotechnology. The specific primer comprises an upstream primer BMF: 5'-GCTACAACGCCGAAATGACC-3' and a downstream primer BMR: 5'-GACGTTGCCCAATACCAAGC-3'. The detection primer and the detection method provided by the invention can be used for detection and identification of banana cladosporium cucumerinum pure culture and also can be used for detecting the banana leaves and fruit; the detection primer provided by the invention is high in specificity and sensitivity; the detection method is high in practicability and simple and convenient in operation; the molecular detection primer can be used for realizing the early detection for banana cladosporium cucumerinum and effectively identifying the similar anthracnose, curvularia disease, black spot, spore leaf spot disease and dark agaricus leaf spot disease of banana, early warning, preventing and controlling the banana cladosporium cucumerinum; the molecular detection primer has significance in preventing and treating disease spreading.

The invention discloses a method for detecting a trichinella circulating antigen by utilizing IgY-McAb sandwich ELISA (enzyme-linked immnuo sorbent assay). With an anti-trichinella muscle larval ES antigen egg yolk antibody IgY as a capture antibody and an anti-trichinella ES antigen McAb as a detection antibody, the preparation method of the IgY comprises the following steps of: adding the trichinella muscle larval into a culture medium to carry out sterile culture, purifying and dialyzing supernate and then concentrating and drying to obtain the ES antiagen, determining protein concentration and then immunizing roman legehenne by utilizing the ES antigen, collecting the IgY from the produced egg yolk by adopting a saturate ammonium sulphate precipitation method, purifying and determining concentration, and sub-packaging for later use; and the preparation method of the McAb comprises the following steps of: establishing a hybridoma cell strain secreting the anti-trichinella muscle larval ES antigen, cloning by adopting a limiting dilution method, purifying McAb by applying an octanoic acid-ammonia sulphate method, determining the concentration and sub-packaging for later use. The invention has the advantage that a new method which has high sensitivity and specificity and has wide application prospect is provided for early detection of trichinella CAg in blood serum of an infected animal.

The invention belongs to the field of immunodetection, and discloses two hybridoma cell strains secreting anti-serine phosphorylation tau protein monoclonal antibodies, and the hybridoma cell strains used for secreting p-Tau 396, 404 monoclonal antibodies, the hybridoma cell strains are preserved in the China Center for Type Culture Collection (CCTCC), and the preservation numbers are respectively CCTCC NO: C2020219 and CCTCC NO: C2020218. According to the invention, two hybridoma cell strains capable of secreting novel p-Tau 396, 404 monoclonal antibodies are provided, and the monoclonal antibodies capable of specifically recognizing human p-Tau 396, 404 and aggregates thereof can be prepared by using the hybridoma cell strains, so that the problem of lack of antibodies capable of simultaneously targeting p-Tau 396, 404 at present is solved. The two monoclonal antibodies provided by the invention can be further applied subsequently to prepare a high-specificity and high-sensitivity p-Tau 396, 404 detection kit, the immunological treatment of specific targeting p-Tau 396, 404 is developed, the disease progress is slowed down, and the blank of the current immunological treatment of targeting p-Tau 396, 404 is filled up.