A fluorescence sensor for detecting macrophage phenotype in two channels and a preparation method and application thereof
By designing a dual-channel fluorescence sensor and assembling gold nanoparticles with fluorescent compounds, real-time and dynamic monitoring of M1 and M2 macrophages can be achieved, solving the problem of difficulty in distinguishing M1 and M2 phenotypes in existing technologies and supporting early detection and efficacy evaluation of various diseases.
Patent Information
- Application Number
- CN202610440203.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-04-03
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies make it difficult to monitor the phenotypes of M1 and M2 macrophages in real time and dynamically in living cells, and traditional methods are cumbersome and time-consuming, making them unsuitable for in vivo imaging diagnosis.
A dual-channel fluorescence sensor was designed, which utilizes gold nanoparticles assembled with fluorescent compounds A and B. The fluorescence signal is switched on and off via NO and Arg-1 reaction to detect M1 and M2 macrophages respectively. The fluorescence sensor achieves rapid quantitative performance through targeted response.
It achieves high specificity and high contrast for real-time synchronous differentiation of M1 and M2 macrophage phenotypes, and can dynamically monitor macrophage phenotypes in solution, cells and ex vivo lesions, supporting early disease detection and efficacy evaluation.
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