Arch insect circulating antigen immunity gold mark fast detecting reagent and method for producing the same

A technology for detecting reagents and antigen immunization, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of long retention time, differentiation, and detection of Toxoplasma gondii antibody, which cannot achieve early diagnosis, and achieves simple operation and wide application range , The effect of convenient storage and transportation

Inactive Publication Date: 2008-09-24
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0002] The existing method for detecting toxoplasmosis is mainly to detect toxoplasma antibody, which appears relatively late in humans and animals after being infected with toxoplasma, and detection of toxoplasma antibody cannot achieve early diagnosis; and toxoplasma antibody in humans and animals The retention time is long, and some animals can persist for life, so the detection of Toxoplasma gondii antibody is difficult to distinguish current infection from past infection

Method used

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  • Arch insect circulating antigen immunity gold mark fast detecting reagent and method for producing the same

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Embodiment 1

[0020] (1) Preparation of Toxoplasma gondii metabolism and secretion antigen: on January 28, 2007, 20 mice with a body weight of 20 grams were inoculated intraperitoneally with 1×10^6 Toxoplasma gondii tachyzoites / only. On January 31, the mice appeared mentally Depression, rough coat and other symptoms, the mice were killed by taking off their necks, and then soaked in 75% alcohol to disinfect the body surface. Each mouse was washed with 2 ml of PH7.2 0.15mol / L PBS buffer solution to collect worms, and a total of 45 ml of peritoneal fluid was collected. The collected peritoneal fluid was centrifuged at 3000 rpm for 40 minutes, and the supernatant was collected. , put the supernatant in a refrigerator at -20°C, take out the supernatant from the refrigerator on February 1, centrifuge three times at a speed of 4000 rpm for 30 minutes each time after melting, collect the supernatant, and the supernatant is ready For the metabolism and secretion of antigens by Toxoplasma gondii, pu...

Embodiment 2

[0027] (1) Preparation of Toxoplasma metabolic secretion antigen: On February 28, 2007, 20 mice weighing 25 grams were intraperitoneally inoculated with 1 × 10^5 Toxoplasma gondii tachyzoites. On March 4, the mice appeared mentally Symptoms such as depression and rough coat, the mice were killed by taking off their necks, and soaked in 70% alcohol to disinfect the body surface. Each mouse was washed with 3 ml of PH7.2 0.15mol / L PBS buffer solution to collect worms, a total of 45 ml of peritoneal fluid was collected, and the collected peritoneal fluid was centrifuged at 4000 rpm for 30 minutes to collect the supernatant , put the supernatant in a refrigerator at -15°C, take out the supernatant from the refrigerator on March 6, centrifuge 4 times at a speed of 3000 rpm after melting, and centrifuge for 40 minutes each time, collect the supernatant, and the supernatant is ready For the metabolism and secretion of antigens of Toxoplasma gondii, put them in a -15°C refrigerator for...

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Abstract

A rapid detection reagent of a toxoplasma circulating antigen immune colloidal gold marker comprises a sample pad, a bonding pad, a cellulose nitrate film, a water absorbent pad and a PVC back lining, wherein, one end of the PVC back lining is sequentially adhered with the sample pad, the bonding pad, the cellulose nitrate film and the water absorbent pad, the bonding pad is coated by a colloidal gold bonder with an anti-toxoplasma polyclonal antibody, the anti-toxoplasma polyclonal antibody and a toxoplasma metaboly secrete antigen are linearly coated on the cellulose nitrate film. The toxoplasma circulating antigen immune colloidal gold marker rapid detection reagent is prepared by preparing the anti-toxoplasma polyclonal antibody, preparing the toxoplasma metaboly secrete antigen, preparing the colloidal gold, preparing a colloidal gold marker of the anti-toxoplasma polyclonal antibody; the colloidal gold marker of the anti-toxoplasma polyclonal antibody is coated on the colloidal gold bonding pad, the anti-toxoplasma polyclonal antibody and the toxoplasma metaboly secrete antigen are linearly coated in a detection region and a stagnant control region of the cellulose nitrate film and the thorough drying and other processes are carried out. The rapid detection reagent of the toxoplasma circulating antigen immune colloidal gold marker can detect the toxoplasma circulating antigens of people and various animals, which has accurate sensitivity and simple operation, and being safe and stable.

Description

technical field [0001] The invention relates to an immune colloidal gold reagent for detecting toxoplasma circulating antigen, in particular to a toxoplasma circulating antigen immune gold label rapid detection reagent strip, and the invention also includes a preparation method of the reagent. Background technique [0002] The existing method for detecting toxoplasmosis is mainly to detect toxoplasma antibody, which appears relatively late in humans and animals after being infected with toxoplasma, and detection of toxoplasma antibody cannot achieve early diagnosis; and toxoplasma antibody in humans and animals The retention time is long, and some animals can persist for life, so the detection of Toxoplasma gondii antibody is difficult to distinguish current infection from past infection. Contents of the invention [0003] The purpose of the present invention is to provide a kind of circulating antigen of toxoplasma gondii that can detect human and various animals at the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543G01N33/532
Inventor 张德林王艳华李学瑞才学鹏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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