39 results about "Trichinella species" patented technology

The invention provides a kit for detecting cysticercus cellulosae, swine trichinella and swine toxoplasmosis. The kit comprises sterile deionized water, polymerase chain reaction (PCR) liquid, thermus aquaticus deoxyribonucleic acid (Taq DNA) polymerase, a GoldView DNA dye, bromophenol blue sample loading buffer solution, a standard substance and a reference substance. In the detection kit provided by the invention, DNA of the cysticercus cellulosae, the swine trichinella and the swine toxoplasmosis in a specimen to be detected can be detected quickly and accurately only by one reaction, and the kit can also be used for the molecular epidemiological survey and curative effect monitoring of cysticercus cellulosae, swine trichinella and swine toxoplasmosis infection. The method has simple preparation steps and is low in cost, easy to operate, time-saving and labor-saving, a large number of reagents and consumables are saved, and the work efficiency is improved. The method is high in detection sensibility, specificity and accuracy, and the false positive rate is reduced.

The invention discloses a preparation method of a recombinant protein and in particular relates to a TsSerpin recombinant protein and application thereof. The preparation method of the TsSerpin recombinant protein comprises the following steps: carrying out reverse transcription, with total RNA (Ribonucleic Acid) extracted from pig trichina muscle larva as a template and Oligo(dT)18Primer as a primer; with the obtained reverse transcription Cdna (Deoxyribose Nucleic Acid) as a template and SEQ ID No.1 and SEQ ID No.2 as primers, carrying out PCR (Polymerase Chain Reaction) amplification, to obtain pig trichina TsSerpin gene; and carrying out the treatment including recombinant plasmid construction, escherichia coli conversion and the like to obtain target protein. The recombinant protein disclosed by the invention can be used for preparing an antigen for detecting trichinosis.

The invention provides a preparation method of a cervical carcinoma cell associated trichina antigen antibody, and also provides medical application of the antibody. Tumor cells are relatively sensitive to a foreign identifier, the cervical carcinoma cell associated trichina antigen can generate strong specific and nonspecific immune reactions on tumor, and simultaneously, the tumor cell associated trichina antigen is easy to prepare and industrialize.

The invention discloses a method for detecting a trichinella circulating antigen by utilizing IgY-McAb sandwich ELISA (enzyme-linked immnuo sorbent assay). With an anti-trichinella muscle larval ES antigen egg yolk antibody IgY as a capture antibody and an anti-trichinella ES antigen McAb as a detection antibody, the preparation method of the IgY comprises the following steps of: adding the trichinella muscle larval into a culture medium to carry out sterile culture, purifying and dialyzing supernate and then concentrating and drying to obtain the ES antiagen, determining protein concentration and then immunizing roman legehenne by utilizing the ES antigen, collecting the IgY from the produced egg yolk by adopting a saturate ammonium sulphate precipitation method, purifying and determining concentration, and sub-packaging for later use; and the preparation method of the McAb comprises the following steps of: establishing a hybridoma cell strain secreting the anti-trichinella muscle larval ES antigen, cloning by adopting a limiting dilution method, purifying McAb by applying an octanoic acid-ammonia sulphate method, determining the concentration and sub-packaging for later use. The invention has the advantage that a new method which has high sensitivity and specificity and has wide application prospect is provided for early detection of trichinella CAg in blood serum of an infected animal.

The invention discloses a fluorescence immunochromatography test strip capable of detecting early infection of pig trichina and a production method thereof, and belongs to the technical field of serological detection. In order to detect trichina infection more quickly, conveniently and sensitively, the test strip comprises a sample pad, a combination pad, a chromatography membrane, a water absorption pad and a bottom plate, and a fluorescent probe is sprayed on the combination pad. The fluorescent probe comprises a time-resolved fluorescent microsphere coupled goat anti-rabbit IgG serving as an indication probe and a time-resolved fluorescent microsphere coupled trichina ES mixed antigen serving as a capture probe, wherein the trichina ES mixed antigen consists of a trichina muscle larva excretion secretion ML-ESP antigen and a trichina intestinal muscle larva excretion secretion iML-ESP antigen; and a detection line and a quality control line are arranged on the chromatography membrane, mouse anti-pig IgG is sprayed on the detection line, and rabbit anti-goat IgG is sprayed on the quality control line. The test strip can be used for on-site rapid detection of trichina.

The invention aims to provide an sHSPs recombinant invasive lactobacillus plantarum live vector DNA vaccine. Lactobacillus is selected as a vector to construct the recombinant invasive lactobacillus plantarum. The lactobacillus plantarum has immunoregulation ability and carries tumor antigen to inhibit tumor growth, and trichina sHSPs as a heteroantigen can break immune tolerance, such that obvious anti-tumor immunity is generated. The lactobacillus plantarum carries the related antigen gene sHSPs so as to provide non-specific immunoregulation ability of the lactobacillus, and the sHSPs can be delivered to a host cell to express the related antigen sHSPs so as to induce a specific immune response. The recombinant lactobacillus plantarum carrying the trichina sHSPs gene exert non-specific immunoregulation ability of the lactobacillus and can also induce specific anti-tumor immunity. Meanwhile, as a probiotic, the lactobacillus has high safety, can be orally taken, and is convenient to use and easy to popularize.

The invention discloses a competitive monoclonal antibody-based trichinosis antibody detection kit and a preparation method thereof, and belongs to the technical field of fluorescence immunoassay. Theinvention discloses a method for detecting trichina infection more quickly, stably and accurately. The invention provides the competitive monoclonal antibody-based trichinosis antibody detection kit,a test strip comprises a sample pad, a chromatography membrane, a water absorption pad and a bottom plate, the chromatography membrane is provided with a detection line and a quality control line, the detection line is sprayed with a rabbit anti-Ts-WN10 polyclonal antibody, and the quality control line is sprayed with rabbit antimouse IgG; a competitive reagent contains a monoclonal antibody Ts-WN10-1H9 secreted by a hybridoma cell strain WN10-1H9 and a recombinant antigen Ts-WN10; and the microbiological preservation number of the hybridoma cell strain WN10-1H9 is CGMCC No. 18316. The kit prepared by the invention is high in specificity and sensitivity, and can be used for quickly detecting the swine trichinopathy.

The invention relates to the field of immunology, and particularly relates to a B cell epitope polypeptide of a serine protease inhibitor (Ts-WM5) with high expression in the larvae phase of trichinamuscle, a hybridoma cell strain, a monoclonal antibody and application thereof. The hybridoma cell strain capable of secreting an anti-Ts-WM5 protein specific antibody is obtained, and the Ts-WM5 protein specific B cell epitope polypeptide recognized by the monoclonal antibody secreted by the hybridoma cell strain is identified, so that the B cell epitope polypeptide has important significance indiagnosis of trichinosis and has important significance in establishing sandwich ELSIA for competitive ELISA and detection of circulating antigens for detection of multiple hosts of trichina and development of subunit vaccines.

Trichinella can be detected in a tissue extract sample. A suitable kit includes (a) a detection carrier containing a first antibody against one or more antigens of Trichinella, and (b) (i) a second antibody against one or more antigens of Trichinella, wherein the second antibody is bound to a signal molecule, or (b) (ii) contains one or more antigens of Trichinella bound to a signal molecule, wherein the antigens of (b) (ii) are configured so as to dissolve binding of the antigens of (a) to the first antibody by competitive displacement.

The invention provides an anti-trichina small heat shock protein (sHSPs) chicken egg yolk antibody and a preparation method thereof, and also discloses a medical application of the egg yolk antibody in tumor resistance. The traditional Chinese medicine composition has the characteristics of enhancing animal immunity, being non-toxic and the like, can be used as an oral preparation medicine for treating tumors, and can be prepared into oral liquid and the like as food for preventing tumors; the egg yolk antibody for poultry such as chicken can be orally taken, is convenient to use, non-toxic, low in cost, safe and convenient for large-scale industrialized production.

The invention provides a recombinant TsPKA-C protein and application thereof and particularly relates to application of the recombinant TsPKA-C protein as taenia solium/cysticercerci detection antigen and application of the recombinant TsPKA-C protein to preparation of a porcine cysticercosis detection kit. The recombinant TsPKA-C protein can specifically detect taenia solium/cysticercerci and has no cross reaction with porcine thin-neck cysticercerci, trichinella sui and the like.

The invention discloses a trichina antibody double-hole rapid detection card and a detection method, the trichina antibody double-hole rapid detection card comprises a PVC base plate, a water absorption pad, a nitrocellulose membrane, a marker pad, a sample pad, a filter substance pad and a dilution pad which are installed in a shell, the nitrocellulose membrane is fixed on the PVC base plate, the left end of the nitrocellulose membrane is in lap joint with the water absorption pad, the right end of the nitrocellulose membrane is in lap joint with the dilution pad, a marker pad is arranged at the right position of the middle of the nitrocellulose membrane, a sample pad is arranged on the marker pad, a detection line and a quality control line are arranged between the water absorption pad and the marker pad on the nitrocellulose membrane, and a window hole, a sample adding hole, a cleaning hole and a quantitative marking line are formed in the shell. A sample is added through the middle sample adding hole, and a diluent is added through the rear cleaning hole, so that the sensitivity and the precision of a detected object can be improved, and the characteristics of rapidness, simplicity and convenience of a rapid diagnosis detection method are kept.

The invention provides an application of recombinant trichina thioredoxin peroxidase 2 in preparation of an anti-trichina infection vaccine, which belongs to the technical field of immunoregulation, and the recombinant trichina thioredoxin peroxidase 2 can induce a mouse body to generate Th2 type immune response, induce proliferation of CD4<+> T cells of a mouse and convert macrophages into M2 type cells by the recombinant trichina thioredoxin peroxidase 2. The immune response reactions play a main role in the trichinella spiralis infection resisting process; therefore, the recombinant trichina thioredoxin peroxidase 2 as an antigen molecule can be used for preparing an anti-trichina infection vaccine.

The invention relates to a trichina gene Ts27 and a protein encoded by the same and also relates to a recombinant vector and a recombinant cell containing the gene Ts27 and an antibody specifically combined with the protein encoded by the gene Ts27. The invention also relates to an application of the gene Ts27, the protein encoded by the gene Ts27 and the antibody to preparation of a detection reagent for diagnosing trichinization of humans and animals, and a detection kit. After the gene Ts27 recombinant protein is immune to animals, an antibody with a high titer can be generated; and the gene Ts27 recombinant protein can be specifically identified by animal or human serums infected by trichinas.

The invention provides application of triclosan in preparation of a medicine for killing parasitic nematodes, and belongs to the technical field of medicinal chemistry. Triclosan has an obvious lethal effect on trichina in all stages, is low in dosage and high in safety, can be used as one of candidate molecules for treating nematode diseases, and can be used for treating nematode diseases. The important reference value is realized on the development of new nematode killing medicines.

The invention discloses a specific detection antigen for trichinosis and application of the specific detection antigen. The antigen protein is a protein as shown in (a1) or (a2): (a1) a protein with an amino acid sequence as shown in SEQ ID No.1; and (a2) a protein which is obtained by substitution and/or deletion and/or addition of one or more amino acid residues on the amino acid sequence of the protein limited by (a1) and can be specifically combined with a sheep trichinosis serum antibody. According to the specific detection antigen for trichinosis and the application of the specific detection antigen, the detection time is advanced to 48 hours for the first time, the operation is simple and convenient, and the sensitivity and the accuracy are good; and the method established by the invention has good specificity, provides a rapid diagnosis method for epidemiological investigation and disease diagnosis, and can be used for simultaneously detecting serum in different stages of adult stage, newborn larva stage and muscle larva stage of infected trichina.