The invention discloses a chimeric antigen receptor targeting HER2 and an expression vector and application thereof, and belongs to the field of tumor immune drugs. The chimeric antigen receptor targeting HER2 includesa signal peptide, a single-chain antibody targeting HER2, an extended CD8 alpha hinge region, a transmembrane region, an inducible co-stimulater, an intracellular signal peptide, a P2A linker peptide, an IL2 signal peptide, and an anti-PD1 single-chain antibody secretion region, wherein the signal peptide, the single-chain antibody targeting HER2, the extended CD8 alpha hinge region, the transmembrane region, the inducible co-stimulater, the intracellular signal peptide, the P2A linker peptide, the IL2 signal peptide, and the anti-PD1 single-chain antibody secretion region are connected in sequence, the nucleotide sequence of the signal peptide is shown as SEQ ID NO:1in a sequence table, the nucleotide sequence of the single-chain antibody targeting HER2 is shown as SEQ ID NO:2in the sequence table, the nucleotide sequence of the extended CD8 alpha hinge region is shown as SEQ ID NO:3in the sequence table, the nucleotide sequence of the transmembrane region is shown as SEQ ID NO:4in the sequence table,the nucleotide sequence of the intracellular signal peptide is shown as SEQ ID NO:5in the sequence table, the nucleotide sequence of the P2A linker peptide is shownas SEQ ID NO:6 in the sequence table, and the nucleotide sequence of the IL2 signal peptide is shown as SEQ ID NO:7in the sequence table. The chimeric antigen receptor has high antitumor activity while producing fewer cytokines.