48 results about "White Cell" patented technology

The invention discloses a fire scene multi-parameter laser wavelength modulation spectrum detection method and a device thereof, wherein, fire smoke products are treated through a smoke pre-treatment device and then pumped into a white cell through an air extracting pump; a multi-channel laser controller scans and modulates the wavelengths of a plurality of DFB lasers according to the time-sharing and multiplexing working mode, and all waves of laser share an optical fiber and are outputted sequentially through a wave combiner, then are aligned by a fiber collimator and sent to the white cell to detect the fire smoke products; a photoelectric detector converts light intensity signals of laser repeatedly reflected and absorbed by the white cell into electrical signals which are sent to two phase-locking magnifier modules for frequency selecting magnification so as to obtain a fundamental frequency component and a second harmonic component; a multi-channel data acquisition card converts output voltage signals of the phase-locking magnifiers into digital signals which are sent to a micro-computer for real-time data processing, so that the fire scene multi-parameter information containing the oxygen concentration of the fire scene, concentrations of various toxic gases and smoke concentration is obtained. The detection method and device can realize real-time on-line monitoring to fire scene multi-parameters, and have the advantages of being real-time, multi-component and highly sensitive, good selectivity of gas, high reliability and strong environmental disturbance resistance ability.

The present invention embodies a technique, referred to as Secure QR Codes, which not only provides aesthetically enhanced QR codes but also allows for security. It can embed a standard black and white QR code, referred to as a public QR code, and a secret QR code, both into a secure QR code. The secure QR code produced is composed of colored cells. The public black and white QR code must first be, either aesthetically enhanced into an enhanced colored QR code, or transformed into a colored QR code with cells of uniform color obtained by transforming each of the black and white cells of the public QR code into cells that takes a color from a subset of possible colors, such that the luminance of each colored cell approximates accurately the black or white luminance values of the public QR code.

This disclosure describes single-use test cartridges, cell analyzer apparatus, and methods for automatically performing microscopic cell analysis tasks, such as counting blood cells in biological samples. A small unmeasured quantity of a biological sample such as whole blood is placed in the disposable test cartridge which is then inserted into the cell analyzer. The analyzer isolates a precise volume of the biological sample, mixes it with self-contained reagents and transfers the entire volume to an imaging chamber. The geometry of the imaging chamber is chosen to maintain the uniformity of the mixture, and to prevent cells from crowding or clumping, when it is transferred into the imaging chamber. Images of essentially all of the cellular components within the imaging chamber are analyzed to obtain counts per unit volume. The devices, apparatus and methods described may be used to analyze a small quantity of whole blood to obtain counts per unit volume of red blood cells, white blood cells, including sub-groups of white cells, platelets and measurements related to these bodies.

The invention discloses a whole blood quality control material. The whole blood quality control material comprises three cell simulating materials, an anticoagulation agent, white cell stationary liquid, and red cell and blood platelet stationary liquid. The invention further discloses a preparation method of the whole blood quality control material. The whole blood quality control material is wide in raw material resources and simple in preparation process; three classes of white cells can be clearly identified on a three-class blood cell instrument; the size difference of red cells and blood platelets is obvious, and the red cells and the blood platelets are easy to identify by the instrument; interferences of the small red cells on the blood platelets are small and the storage life can reach 6 months; the use requirements of clinical quality control are met.

The invention relates to a method for quickly and efficiently extracting whole blood genome DNA (deoxyribonucleic acid), and belongs to the field of nucleic acid purification. The method comprises the following steps: adding a red cell lysis solution into whole blood, eddying until red cells are completely split, removing supernatant, and sucking all residual liquid; adding a white cell lysis solution and protease K, and splitting white cells in a water bath of 37 DEG C until the white cells are deposited and dissolved; adding sodium chloride and chloroform, carrying out centrifugal separation on DNA, and transferring the supernatant to another clean EP (epoxy) pipe; adding ammonium acetate and absolute ethanol for depositing DNA, washing by 75% of ethanol, naturally drying, and adding TE (Tris-EDTA(ethylene diamine tetraacetic acid)) for dissolving to obtain DNA. The method has the characteristics of high speed, high efficiency and non-toxicity. The extracted DNA can be used for various subsequent molecular biology studies such as polymerase chain reaction (PCR), methylation detection and gene cloning.

The invention relates to a device for classifying and counting white cells by using an even high-order aspherical laser shaping system and belongs to optics. The device comprises a laser shaping and lighting unit, a sample processing and transmission unit and a subsequent signal processing unit, wherein a laser shaping module at least comprises a uniformizing and collimating lens which is used for collimating and uniformizing the Gauss beam emitted by a laser; the sample processing and transmission unit comprises an air pressure transmission control module, a liquid transmission control module and a sheath flow pool; and the subsequent signal processing unit comprises an optical signal processing module, a photovoltaic conversion module and an electrical signal processing module. The device has the advantages that when the Gauss beam emitted by the laser is collimated, the light energy density which is distributed on the cross section of a light beam in a Gauss shape is adjusted to be distributed nearly flatly, so that the scattering light intensity is slightly deviated, and the problems of erroneous judgment and unclear classification limits during subsequent signal processing are solved.

The invention belongs to the technical field of biological engineering and environmental engineering and discloses a low-temperature aerobic denitrifying strain Pseudomonas psychrophila Den-03. The strain is suitable for growing in a neutral culture medium at the pH (potential of hydrogen) of 7.2 and at the temperature of 10 DEG C, and is characterized in that the strain is atrichous, aerobic and capable of achieving denitrification, has yellowish-white cells and can grow with nitrate nitrogen serving as energy, and both catalase and oxidase are positive. The strain obtained by screening solves the problems of low production efficiency, low denitrification efficiency in winter and the like during sewage treatment of northern cold cities, removal rate of TN (total nitrogen) in low-temperature domestic sewage reaches 74%, and on the premise of guaranteeing stable denitrification efficiency, denitrification rate of urban sewage treatment systems in winter is increased, production efficiency is improved, production energy consumption is reduced, and the stain is of great significance to realization of short-range synchronous nitrification and denitrification.

The invention discloses a magnetic nanoparticle and an immunomagnetic nanoparticle with a cell-like structure as well as a preparation method and application thereof. The magnetic nanoparticle with the cell-like structure uses a superparamagnetic Fe3O4 nanoparticle as a core and is wrapped with a white cell membrane on the surface, so that the magnetic nanoparticle has excellent magnetic responseperformance, furthermore, the white cell membrane wrapping on the surface of the magnetic nanoparticle is homologous with white cells, thereby effectively weakening non-specific adsorption to the white cell; an antibody is modified onto the white cell membrane wrapping on the surface of the magnetic nanoparticle through mediating of lipid molecules-polyethylene glycol-biotin molecules and avidin,so as to obtain the immunomagnetic nanoparticle with the cell-like structure. CTCs cells can be enriched and separated with high efficiency and high purity.

The invention discloses a micro-fluidic chip for screening rare cells in whole blood. The micro-fluidic chip comprises a microchannel as well as a platelet removing region formed on the microchannel by a first interdigital electrode assembly, a red cell lysis region formed by a lysis pipeline segment on the microchannel, a red cell removing region formed on the microchannel by a second interdigital electrode assembly, a white cell removing region formed on the microchannel by a magnetic field and a target cell extraction region formed on the microchannel through a third interdigital electrodeassembly in sequence. According to the micro-fluidic chip disclosed by the invention, platelets and lysed red cells are removed by using the size difference; white cells are removed by using a specific monoclonal antibody and a magnetic bead mode, and screening purity and capture ratio of target cells are favorably improved; in addition, the micro-fluidic chip can clean samples. The system disclosed by the invention does no harm to cells, can be used for conventional flow cell sorting and screening the rare cells and has important significance for promoting noninvasive prenatal screening and tumor prognosis detection.

The invention discloses composite polysaccharide for resisting radiation. Effective ingredients of the composite polysaccharide comprise lentinan, agaricus blazei polysaccharide and tuckahoe polysaccharide. The composite polysaccharide can obviously improve the immunity of mice, can enhance the white cell number and the bone marrow cell DNA content of the mice after gamma-ray irradiation, has stronger radiation resisting effect, and is particularly suitable for tumor disease radiation therapy and chemotherapy or adjuvant therapy after tumor disease operation.

The invention discloses a particle swarm optimization ITTI model-based white cell region extraction method and belongs to the image processing technical field. According to the method, firstly, direction, brightness and color saliency features of an original grayscale map are extracted through using Gaussian filtering and multiscale normalization; secondly, adaptive coefficient fusion is performed on the three kinds of saliency features based on a principle that the contributions of eyes to visual characteristics are inconsistent, so that a saliency map can be obtained; and finally, region of interest extraction is performed on the saliency map through using an improved particle swarm optimization algorithm-based Otsu method, so that a complete white cell region can be obtained. As indicated by experiments, the method of the invention can better extract a complete white cell region compared with other methods for extracting regions of interest of a bone marrow cell image.

A method and an apparatus for processing blood in a closed system in order to obtain fractionation of the corpusculate part and preferably the separation of the white cells from the red cells according to their sedimentation rate and maintain a high degree of vitality of the cells. The white blood cells are sequestered from the remaining cell population of the blood by placing the blood in a container, preventing its coagulation and separating the blood by means of a sedimentation agent in at least two compartments, one of which is extremely rich in white cells.

An optical beam combiner for combining a plurality of light beams comprises: a plurality of spherical mirrors; and a flat mirror, the plurality of spherical mirrors and the flat mirror configured to form at least one multiple pass light beam optical arrangement for receiving the plurality of light beams and for superimposing spot images of the light beams onto a single location with a single incident angle. In addition, a waveguide-based optical White cell comprises: a waveguide having front and rear edges, the inside surfaces thereof being coated with a reflective material, wherein the front edge including an input section for the passage of at least one light beam into the waveguide; at least one waveguide lens disposed in front of the inside surface of the rear edge to form a plurality of waveguide spherical mirrors at the rear edge; a plurality of angled micro mirrors disposed at the inside surface of the front edge; and the plurality of waveguide spherical mirrors and the coated front edge configured to form at least one waveguide White cell.

The invention provides a method for preparing a DNA library and specifically capturing and a kit, and the method is suitable for constructing the DNA library of cfDNA, white cell gDNA and DNA from a tissue sample as well as hybridizing and capturing a special target gene area. In the preparation method for the DNA library, terminal repair and 3' end A addition are performed in the same reaction system, and hybridization input is improved, and a blocking preparation in hybridizing and capturing is optimized, so that higher capturing efficiency, higher library variety and more uniform coverage degree are obtained, and therefore, the preparation method is especially suitable for constructing a trace sample library with low-abundance mutation, and is beneficial for realizing saving in technical process. The method provided by the invention can be used for conveniently realizing all variations such as mutation, deletion, insertion, fusion as well as detection on copy number amplification of a target gene, and overcomes the defects that target gene sequences are enriched through a PCR method on DNA level at present, mutation and deletion of the target gene only can be detected, and fusion cannot be detected.

The invention discloses a nu-support vector machine-based white cell classification method. The method comprises the steps of firstly preprocessing a color blood microscopic image by adopting a medianfiltering method; secondly mapping an initial color space to an HLS color space, thereby obtaining a hue image after conversion; thirdly performing coarse segmentation on the hue image by using a nu-support vector machine-based grayscale image segmentation method, and detecting out all white cells by adopting a layer screening policy and a mathematical morphology method; fourthly performing finesegmentation on each white cell image, namely finishing separation of cell nuclei, cytoplasm and a background, and extracting most representative 47 features for each white cell, the cell nuclei and the cytoplasm; and finally finishing white cell classification by virtue of a nu-support vector machine. The usage performance of a whole white cell automatic identification and counting system can beremarkably improved.

The invention provides a distance conversion-based shape characteristic description method. The method comprises the following steps of: performing distance conversion on an image, and recording a distance value of each point and corresponding vector information; and then calculating shape characteristic parameters such as integral length, integral acute angle, integral obtuse angle, edge length, edge acute angle, edge obtuse angle and the like by using a half of the maximum distance value as a threshold value according to the method provided by the invention. The shape characteristic parameters can reflect the characteristics of a shape on different scales and provide more detailed information for identification of cell nucleuses so that the method has good separable property on white cells with different nuclear shapes.

A blood cell collection system having means for detecting when a cell separation chamber has filled with white blood cells, and flushing the cells out of the cell separation chamber into a collect bag. A red-green sensor senses the optical characteristics of fluid leaving the cell separation chamber. A baseline value is calculated. The device calculates a ratio of the intensities of red light and green light and a peak-to-peak ratio of intensities. If either ratio exceeds thresholds computed from the baseline, the device flushes the cells into the collect bag. A camera detects white cells passing into the cell separation chamber and the device calculates the number of cells being collected. If the calculated number of collected cells exceeds a certain limit, the cell separation chamber is flushed. If the device is unable to establish a baseline, the donation can proceed, relying solely on the calculated number of collected cells.

The invention provides an active pixel cell structure and a method of preparing the same on a substrate, so as to enable reduction of dark current and white cell counts for active pixel cells. The process of preparing active pixel cell structures introduces stress on the substrate, which could lead to increased dark current and white cell counts of active pixel cells. By depositing a stress layeras part of a pre-metal dielectric layer with a stress that counters the stress induced, both the dark current and the white cell counts can be reduced. If the transistors of the active pixel cells are NMOS, the carrier mobility can also be increased by a tensile stress layer. Raman Spectroscopy can be used to measure the stress exerted on the substrate prior to the deposition of the stress layer.The invention can reduce the dark current and white cell counts for the active pixel cells.

The invention provides a gas shooting detection system. The gas shooting detection system comprises a gas mixing regulation device, a gas chamber device, a light source, a spectrometer and a shootingdevice, wherein the gas mixing regulation device comprises a flow regulator, an inert gas humidity generator, a gas mixer, a to-be-detected gas supply bottle and an inert gas supply bottle; the gas chamber device comprises a gas absorption detection pool; a gas inlet of the gas chamber device is connected with a gas outlet of the gas mixing regulation device; the gas absorption detection pool comprises a light inlet hole, a white cell structure, a light outlet hole and a camera shooting port; a light path converter is arranged at the positions of the light outlet hole and the camera shooting port; the shooting device comprises a filter and a camera; the filter comprises a plurality of filter sheets arranged on a filter wheel; and the shooting device is connected with the camera shooting port. The gas shooting detection system provided by the invention also can implement detection on pollution gas with different concentrations and different humidity degrees, and is helpful for establishing a pollution gas shooting detection data model.

Provided are applications of arctigenin in formulating medicines for preventing or treating diseases related to blood cell reduction, and preparations that contain arctigenin, particularly micro-emulsions that contain arctigenin. Experiments show that arctigenin can significantly increase the white cell counts after chemotherapy, especially the neutrophil granulocyte counts.

The present invention provides a colloidal turbinate polysaccharide and its preparation method. Said method includes the following steps: pulverizing colloidal turbinate or its mycelium, decocting it by adding water, filtering to obtain filtrate, combining filtrates, concentrating, standing still, cooling to room temp., making the above-mentioned material pass through active carbon column twice, collecting effluent liquor, concentrating, adding ethyl alcohol to make precipitation, standing still, filtering, adding proper quantity of ethyl alcohol to make elution, reduced pressure drying the obtained precipitate so as to obtain the invented colloidal polysaccharide which can be used as main medicine or can be used for forming medicine prescription together with other medicines, and can be used for resisting tumor, increaisng white cell, resisting sore, resisting hepatitis, raising immunity and reducing blood fat.

The invention discloses a special bacterial genome DNA extraction kit and method for septicemia. The kit consists of a buffer solution GPA, a buffer solution GRB, a bacterial lysis solution DA, a bacterial lysis solution DB, a buffer solution DC, a washing solution PE, an eluent EB and protease K. According to the extraction kit and method provided by the invention, red / white cells are separated by virtue of the buffer solution GRB, so that the influence and interference of human genome can be removed to the greatest extent; bacterial cells are cracked and broken by virtue of SDS and the protease K, and protein is degraded, so that bacterial genome DNA is released; and by virtue of a silica gel column separation and purification method, the bacterial genome DNA with high quality can be obtained.

The present invention embodies a technique, referred to as Secure QR Codes, which not only provides aesthetically enhanced QR codes but also allows for security. It can embed a standard black and white QR code, referred to as a public QR code, and a secret QR code, both into a secure QR code. The secure QR code produced is composed of colored cells. The public black and white QR code must first be, either aesthetically enhanced into an enhanced colored QR code, or transformed into a colored QR code with cells of uniform color obtained by transforming each of the black and white cells of the public QR code into cells that takes a color from a subset of possible colors, such that the luminance of each colored cell approximates accurately the black or white luminance values of the public QR code.

The invention discloses a white cell type flame atomizer which comprises three concave surface reflectors M1, M2 and M3 with the same focal distance, wherein the concave surface reflectors M1 and M2 are completely same in size and are fixed at one end of a full titanium optical table in parallel, the concave surface reflector M3 is fixed at the other end of the full titanium optical table, the concave surfaces of the concave surface reflectors M1 and M2 are opposite to the concave surface of the concave surface reflector M3, the distance between the concave surface reflectors M1 and M2 and the concave surface reflector M3 is equal to the radius of curvature of the concave surface reflector with the focal distance, a full titanium burning sheet is fixed on the full titanium optical table between the concave surface reflectors M1 and M2 and the concave surface reflector M3, and the full titanium optical table is welded on a full titanium burning seat. According to the invention, a light source is reflected for multiple times by the three concave surface reflectors and goes through 12 linear flames, so that the utilization rate of the light is increased, and the flame atomizer is more miniaturized.

The invention discloses a poria lamb liver polypeptide composition and application thereof, and relates to the technical field of polypeptide application. The poria lamb liver polypeptide compositionis prepared from the raw materials in parts by weight: 20-30 parts of poria, 24-30 parts of lamb liver, 10-20 parts of almond, 3-8 parts of corn, 6-10 parts of glucose, 3-8 parts of lactose, 5-9 partsof ox bone, 3-5 parts of fruit juice, 9-15 parts of wolfberry and 5-17 parts of ginseng. According to the poria lamb liver polypeptide composition and the application thereof, blood sugar can be lowered by adding the poria and the lamb liver, anti-tumor is achieved, metabolism is promoted, night blindness and vision loss can be prevented, vitamin A and phosphorus are rich, the composition is suitable for patients with deficiency of a body, enriching yin and nourishing kidney, tonifying qi and calming the nerves can be achieved by adding the wolfberry and the ginseng, the immune function of cells can be enhanced by adding the ox bone, the generation, increment and differentiation of bone marrow white cells are promoted, the human immunity is enhanced, skin aging is delayed, low-density cholesterol is advantageously lowered by adding the almond and the corn, the insulin activity is improved, and diabetes can be prevented well.

The invention discloses a hydrate formation monitoring system based on optics. The system comprises a tracer gas introduction unit, a hydrate formation monitoring unit, an analytic unit, a temperature and pressure detection unit, an acquisition unit and a data processing unit, wherein the hydrate formation monitoring unit comprises a laser emitter and a breathable gas chamber formed in a reactor unit; a white cell used for absorbing laser by gas, a first self-focusing lens used for collimating and coupling laser emitted into the gas chamber, and a second self-focusing lens used for collimating and coupling laser reflected in the white cell and emitting the laser from the gas chamber are arranged in the gas chamber. The invention further provides a hydrate formation monitoring method based on optics. According to the hydrate formation monitoring method and system, by introducing a certain amount of the tracer gas which easily forms hydrate into the reactor unit, detecting the concentration change of the tracer gas before and after reaction in the reactor unit and combining the temperature and pressure change, whether the hydrate is formed in the reactor unit or not is judged, so that a monitoring result is relatively reliable and the precision is relatively high.